In Vitro Functional Reactivities of Cutaneous Mast Cells From Patients With Mastocytosis
Cutaneous mast cells from 3 patients with mastocytosis were evaluated for their morphologic characteristics and in vitro functional reactivities to different secretory agonists. By electron microscopy, mastocytosis mast cells appeared larger than normal skin mast cells, frequently had atypical, high...
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| Veröffentlicht in: | Journal of investigative dermatology 1987-09, Vol.89 (3), p.264-268 |
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| creator | Tharp, Michael D. Chaker, Basem Glass, Marcia J. Burton, Rebecca Seelig, Leonard L. |
| description | Cutaneous mast cells from 3 patients with mastocytosis were evaluated for their morphologic characteristics and in vitro functional reactivities to different secretory agonists. By electron microscopy, mastocytosis mast cells appeared larger than normal skin mast cells, frequently had atypical, highly indented or bibbed nuclei, and each contained numerous, elongated cytoplasmic projections. Suspensions of mastocytosis mast cells were obtained from lesional skin biopsy specimens, and their response to both immunologic and nonimmunologic secretagogues was compared with mast cells from normal skin. Lesional skin mast cells had a net histamine release of 12.3% (± 1.3 SEM) and 31.1% (± 6.0 SEM) following stimulation with the purified human anaphylotoxin C3a and mouse monoclonal antihuman IgE antibodies, respectively. This specific release was similar to the responses observed in normal skin mast cells (11.5% ± 4.5 SEM and 16.7% ± 2.1 SEM, respectively. Mast cells from cutaneous lesions of mastocytosis also responded to the nonimmunologic secretagogues, morphine sulfate and calcium ionophore A23187 with a specific histamine release of 15.I% (± 1.2 SEM) and 39.8% (± 8.7 SEM), respectively. The results of this study demonstrate that mast cells from lesions of mastocytosis are morphologically atypical, but have a histamine content similar to normal skin mast cells and retain their functional reactivities to clinically relevant secretory stimuli. |
| doi_str_mv | 10.1111/1523-1747.ep12471299 |
| format | Article |
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By electron microscopy, mastocytosis mast cells appeared larger than normal skin mast cells, frequently had atypical, highly indented or bibbed nuclei, and each contained numerous, elongated cytoplasmic projections. Suspensions of mastocytosis mast cells were obtained from lesional skin biopsy specimens, and their response to both immunologic and nonimmunologic secretagogues was compared with mast cells from normal skin. Lesional skin mast cells had a net histamine release of 12.3% (± 1.3 SEM) and 31.1% (± 6.0 SEM) following stimulation with the purified human anaphylotoxin C3a and mouse monoclonal antihuman IgE antibodies, respectively. This specific release was similar to the responses observed in normal skin mast cells (11.5% ± 4.5 SEM and 16.7% ± 2.1 SEM, respectively. Mast cells from cutaneous lesions of mastocytosis also responded to the nonimmunologic secretagogues, morphine sulfate and calcium ionophore A23187 with a specific histamine release of 15.I% (± 1.2 SEM) and 39.8% (± 8.7 SEM), respectively. The results of this study demonstrate that mast cells from lesions of mastocytosis are morphologically atypical, but have a histamine content similar to normal skin mast cells and retain their functional reactivities to clinically relevant secretory stimuli.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12471299</identifier><identifier>PMID: 3624898</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Biopsy ; Calcimycin ; calcium ionophores ; Child ; Child, Preschool ; Dermatology ; Electron microscopy ; Hematologic and hematopoietic diseases ; Histamine ; Humans ; Immunoglobulin E ; Infant ; Infant, Newborn ; Male ; Mast cells ; Mast Cells - metabolism ; Mast Cells - pathology ; Mast Cells - ultrastructure ; Mastocytosis ; Mastocytosis - metabolism ; Mastocytosis - pathology ; Medical sciences ; Monoclonal antibodies ; Morphine ; Nuclei ; Other diseases. Hematologic involvement in other diseases ; Skin - pathology ; Skin - physiopathology ; Skin diseases ; Sulfate</subject><ispartof>Journal of investigative dermatology, 1987-09, Vol.89 (3), p.264-268</ispartof><rights>1987 The Society for Investigative Dermatology, Inc</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-f5adea43b2cf58c496df7603171fcabde00b9b2b0eab6fdce266fa48a4e408a3</citedby><cites>FETCH-LOGICAL-c334t-f5adea43b2cf58c496df7603171fcabde00b9b2b0eab6fdce266fa48a4e408a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7582461$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3624898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tharp, Michael D.</creatorcontrib><creatorcontrib>Chaker, Basem</creatorcontrib><creatorcontrib>Glass, Marcia J.</creatorcontrib><creatorcontrib>Burton, Rebecca</creatorcontrib><creatorcontrib>Seelig, Leonard L.</creatorcontrib><title>In Vitro Functional Reactivities of Cutaneous Mast Cells From Patients With Mastocytosis</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Cutaneous mast cells from 3 patients with mastocytosis were evaluated for their morphologic characteristics and in vitro functional reactivities to different secretory agonists. By electron microscopy, mastocytosis mast cells appeared larger than normal skin mast cells, frequently had atypical, highly indented or bibbed nuclei, and each contained numerous, elongated cytoplasmic projections. Suspensions of mastocytosis mast cells were obtained from lesional skin biopsy specimens, and their response to both immunologic and nonimmunologic secretagogues was compared with mast cells from normal skin. Lesional skin mast cells had a net histamine release of 12.3% (± 1.3 SEM) and 31.1% (± 6.0 SEM) following stimulation with the purified human anaphylotoxin C3a and mouse monoclonal antihuman IgE antibodies, respectively. This specific release was similar to the responses observed in normal skin mast cells (11.5% ± 4.5 SEM and 16.7% ± 2.1 SEM, respectively. Mast cells from cutaneous lesions of mastocytosis also responded to the nonimmunologic secretagogues, morphine sulfate and calcium ionophore A23187 with a specific histamine release of 15.I% (± 1.2 SEM) and 39.8% (± 8.7 SEM), respectively. The results of this study demonstrate that mast cells from lesions of mastocytosis are morphologically atypical, but have a histamine content similar to normal skin mast cells and retain their functional reactivities to clinically relevant secretory stimuli.</description><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Calcimycin</subject><subject>calcium ionophores</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Dermatology</subject><subject>Electron microscopy</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Histamine</subject><subject>Humans</subject><subject>Immunoglobulin E</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Mast cells</subject><subject>Mast Cells - metabolism</subject><subject>Mast Cells - pathology</subject><subject>Mast Cells - ultrastructure</subject><subject>Mastocytosis</subject><subject>Mastocytosis - metabolism</subject><subject>Mastocytosis - pathology</subject><subject>Medical sciences</subject><subject>Monoclonal antibodies</subject><subject>Morphine</subject><subject>Nuclei</subject><subject>Other diseases. 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Hematologic involvement in other diseases</topic><topic>Skin - pathology</topic><topic>Skin - physiopathology</topic><topic>Skin diseases</topic><topic>Sulfate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tharp, Michael D.</creatorcontrib><creatorcontrib>Chaker, Basem</creatorcontrib><creatorcontrib>Glass, Marcia J.</creatorcontrib><creatorcontrib>Burton, Rebecca</creatorcontrib><creatorcontrib>Seelig, Leonard L.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tharp, Michael D.</au><au>Chaker, Basem</au><au>Glass, Marcia J.</au><au>Burton, Rebecca</au><au>Seelig, Leonard L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Functional Reactivities of Cutaneous Mast Cells From Patients With Mastocytosis</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1987-09</date><risdate>1987</risdate><volume>89</volume><issue>3</issue><spage>264</spage><epage>268</epage><pages>264-268</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Cutaneous mast cells from 3 patients with mastocytosis were evaluated for their morphologic characteristics and in vitro functional reactivities to different secretory agonists. By electron microscopy, mastocytosis mast cells appeared larger than normal skin mast cells, frequently had atypical, highly indented or bibbed nuclei, and each contained numerous, elongated cytoplasmic projections. Suspensions of mastocytosis mast cells were obtained from lesional skin biopsy specimens, and their response to both immunologic and nonimmunologic secretagogues was compared with mast cells from normal skin. Lesional skin mast cells had a net histamine release of 12.3% (± 1.3 SEM) and 31.1% (± 6.0 SEM) following stimulation with the purified human anaphylotoxin C3a and mouse monoclonal antihuman IgE antibodies, respectively. This specific release was similar to the responses observed in normal skin mast cells (11.5% ± 4.5 SEM and 16.7% ± 2.1 SEM, respectively. Mast cells from cutaneous lesions of mastocytosis also responded to the nonimmunologic secretagogues, morphine sulfate and calcium ionophore A23187 with a specific histamine release of 15.I% (± 1.2 SEM) and 39.8% (± 8.7 SEM), respectively. The results of this study demonstrate that mast cells from lesions of mastocytosis are morphologically atypical, but have a histamine content similar to normal skin mast cells and retain their functional reactivities to clinically relevant secretory stimuli.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>3624898</pmid><doi>10.1111/1523-1747.ep12471299</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of investigative dermatology, 1987-09, Vol.89 (3), p.264-268 |
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| subjects | Biological and medical sciences Biopsy Calcimycin calcium ionophores Child Child, Preschool Dermatology Electron microscopy Hematologic and hematopoietic diseases Histamine Humans Immunoglobulin E Infant Infant, Newborn Male Mast cells Mast Cells - metabolism Mast Cells - pathology Mast Cells - ultrastructure Mastocytosis Mastocytosis - metabolism Mastocytosis - pathology Medical sciences Monoclonal antibodies Morphine Nuclei Other diseases. Hematologic involvement in other diseases Skin - pathology Skin - physiopathology Skin diseases Sulfate |
| title | In Vitro Functional Reactivities of Cutaneous Mast Cells From Patients With Mastocytosis |
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