The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1 α-subunit
A cDNA encoding the Elα subunit of the Arabidopsis thaliana (At) mitochondrial (mt) pyruvate dehydrogenase complex (PDC) was sequenced. The 1435-bp cDNA consists of a 1167-bp open reading frame encoding a 43.0-kDa polypeptide of 389 amino acids (aa) (p I 7.1). The plant Elα subunit has 47–51% aa seq...
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| Veröffentlicht in: | Gene 1995-10, Vol.164 (2), p.251-254 |
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| creator | Luethy, Michael H. Miernyk, Jan A. Randall, Douglas D. |
| description | A cDNA encoding the Elα subunit of the
Arabidopsis thaliana (At) mitochondrial (mt) pyruvate dehydrogenase complex (PDC) was sequenced. The 1435-bp cDNA consists of a 1167-bp open reading frame encoding a 43.0-kDa polypeptide of 389 amino acids (aa) (p
I 7.1). The plant Elα subunit has 47–51% aa sequence identity with other eukaryotic sequences. Among the regions that are highly conserved are the aa surrounding phosphorylation sites 1 and 2 of the mammalian sequence, including the conserved Ser
292 residue of
At at site 1. An essential active site residue, Cys
62 of the bovine subunit, is also conserved. A 32-aa presumptive mt targeting sequence is present at the N terminus. |
| doi_str_mv | 10.1016/0378-1119(95)00465-I |
| format | Article |
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Arabidopsis thaliana (At) mitochondrial (mt) pyruvate dehydrogenase complex (PDC) was sequenced. The 1435-bp cDNA consists of a 1167-bp open reading frame encoding a 43.0-kDa polypeptide of 389 amino acids (aa) (p
I 7.1). The plant Elα subunit has 47–51% aa sequence identity with other eukaryotic sequences. Among the regions that are highly conserved are the aa surrounding phosphorylation sites 1 and 2 of the mammalian sequence, including the conserved Ser
292 residue of
At at site 1. An essential active site residue, Cys
62 of the bovine subunit, is also conserved. A 32-aa presumptive mt targeting sequence is present at the N terminus.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(95)00465-I</identifier><identifier>PMID: 7590338</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis thaliana ; Base Sequence ; Carbon metabolism ; Cattle ; Conserved Sequence ; Cysteine ; Macromolecular Substances ; Mitochondria - enzymology ; Molecular Sequence Data ; Open Reading Frames ; phosphorylation ; plant ; Pyruvate Dehydrogenase Complex - chemistry ; Pyruvate Dehydrogenase Complex - genetics ; Sequence Homology, Amino Acid</subject><ispartof>Gene, 1995-10, Vol.164 (2), p.251-254</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-4f96ba2e9f2e2893d410decbdd448c1c8c68cb884fad8b2fd0bda0ce5f74b3213</citedby><cites>FETCH-LOGICAL-c388t-4f96ba2e9f2e2893d410decbdd448c1c8c68cb884fad8b2fd0bda0ce5f74b3213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(95)00465-I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7590338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luethy, Michael H.</creatorcontrib><creatorcontrib>Miernyk, Jan A.</creatorcontrib><creatorcontrib>Randall, Douglas D.</creatorcontrib><title>The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1 α-subunit</title><title>Gene</title><addtitle>Gene</addtitle><description>A cDNA encoding the Elα subunit of the
Arabidopsis thaliana (At) mitochondrial (mt) pyruvate dehydrogenase complex (PDC) was sequenced. The 1435-bp cDNA consists of a 1167-bp open reading frame encoding a 43.0-kDa polypeptide of 389 amino acids (aa) (p
I 7.1). The plant Elα subunit has 47–51% aa sequence identity with other eukaryotic sequences. Among the regions that are highly conserved are the aa surrounding phosphorylation sites 1 and 2 of the mammalian sequence, including the conserved Ser
292 residue of
At at site 1. An essential active site residue, Cys
62 of the bovine subunit, is also conserved. A 32-aa presumptive mt targeting sequence is present at the N terminus.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Base Sequence</subject><subject>Carbon metabolism</subject><subject>Cattle</subject><subject>Conserved Sequence</subject><subject>Cysteine</subject><subject>Macromolecular Substances</subject><subject>Mitochondria - enzymology</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>phosphorylation</subject><subject>plant</subject><subject>Pyruvate Dehydrogenase Complex - chemistry</subject><subject>Pyruvate Dehydrogenase Complex - genetics</subject><subject>Sequence Homology, Amino Acid</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAQgC1EVZbCG4DkE6KHgJ04icOh0qotsFIFl3K2bM-ka5TYwU4q9kl4Dl6kz4SXXfUIvlij-eZH8xHyirN3nPHmPataWXDOu7ddfc6YaOpi84SsuGy7grFKPiWrR-QZeZ7Sd5ZfXZen5LStO1ZVckV-3W6Rjm4Odhs8RKcHOu3icq9npIDbHcRwh14npDaM04A_P1C_2AHD7ACp9pApWCwC1aPzodDWAU34Y0FvMdHQU03t1Zc1zXEA5-_onAeuozYOwpRcyrEenPaaXnP68LtIi1m8m1-Qk14PCV8e_zPy7eP17eXn4ubrp83l-qawlZRzIfquMbrEri-xlF0FgjNAawCEkJZbaRtpjZSi1yBN2QMzoJnFum-FqUpenZE3h75TDHnpNKvRJYvDoD2GJam2bdqSSfFfkLdMMillBsUBtDGkFLFXU3SjjjvFmdqLU3sram9FdbX6K05tctnrY__FjAiPRUdTOX9xyGO-xr3DqJJ1-yODi2hnBcH9e8Af7xWr0Q</recordid><startdate>19951027</startdate><enddate>19951027</enddate><creator>Luethy, Michael H.</creator><creator>Miernyk, Jan A.</creator><creator>Randall, Douglas D.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19951027</creationdate><title>The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1 α-subunit</title><author>Luethy, Michael H. ; Miernyk, Jan A. ; Randall, Douglas D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-4f96ba2e9f2e2893d410decbdd448c1c8c68cb884fad8b2fd0bda0ce5f74b3213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis thaliana</topic><topic>Base Sequence</topic><topic>Carbon metabolism</topic><topic>Cattle</topic><topic>Conserved Sequence</topic><topic>Cysteine</topic><topic>Macromolecular Substances</topic><topic>Mitochondria - enzymology</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>phosphorylation</topic><topic>plant</topic><topic>Pyruvate Dehydrogenase Complex - chemistry</topic><topic>Pyruvate Dehydrogenase Complex - genetics</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luethy, Michael H.</creatorcontrib><creatorcontrib>Miernyk, Jan A.</creatorcontrib><creatorcontrib>Randall, Douglas D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luethy, Michael H.</au><au>Miernyk, Jan A.</au><au>Randall, Douglas D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1 α-subunit</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1995-10-27</date><risdate>1995</risdate><volume>164</volume><issue>2</issue><spage>251</spage><epage>254</epage><pages>251-254</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A cDNA encoding the Elα subunit of the
Arabidopsis thaliana (At) mitochondrial (mt) pyruvate dehydrogenase complex (PDC) was sequenced. The 1435-bp cDNA consists of a 1167-bp open reading frame encoding a 43.0-kDa polypeptide of 389 amino acids (aa) (p
I 7.1). The plant Elα subunit has 47–51% aa sequence identity with other eukaryotic sequences. Among the regions that are highly conserved are the aa surrounding phosphorylation sites 1 and 2 of the mammalian sequence, including the conserved Ser
292 residue of
At at site 1. An essential active site residue, Cys
62 of the bovine subunit, is also conserved. A 32-aa presumptive mt targeting sequence is present at the N terminus.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7590338</pmid><doi>10.1016/0378-1119(95)00465-I</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1995-10, Vol.164 (2), p.251-254 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence Animals Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis thaliana Base Sequence Carbon metabolism Cattle Conserved Sequence Cysteine Macromolecular Substances Mitochondria - enzymology Molecular Sequence Data Open Reading Frames phosphorylation plant Pyruvate Dehydrogenase Complex - chemistry Pyruvate Dehydrogenase Complex - genetics Sequence Homology, Amino Acid |
| title | The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1 α-subunit |
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