A C-RAS-Ki oncogene is activated, amplified and overexpressed in a human osteosarcoma cell line
We present a characterization of an activated oncogene which we found to be present in DNA of the OHA osteosarcoma cell line. We identify this tumor oncogene which transforms Swiss mouse 3T3-cells, with c-ras-Ki 2, one of two known members of the Kirsten ras family of human proto-oncogenes. Its stru...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1987-07, Vol.146 (2), p.395-402 |
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| container_title | Biochemical and biophysical research communications |
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| creator | NARDEUX, P. C DAYA-GROSJEAN, L LANDIN, R. M ANDEOL, Y SUAREZ, H. G |
| description | We present a characterization of an activated oncogene which we found to be present in DNA of the OHA osteosarcoma cell line. We identify this tumor oncogene which transforms Swiss mouse 3T3-cells, with c-ras-Ki 2, one of two known members of the Kirsten ras family of human proto-oncogenes. Its structural outlines are given and we show that: 1) a single point mutation causing a substitution of valine for glycine in codon 12 was found by DNA sequencing; 2) the c-ras-Ki gene is amplified and overexpressed in the original OHA tumor cells and its transformants and 3) the gene product is an abnormal form of the p21 protein. |
| doi_str_mv | 10.1016/0006-291X(87)90542-0 |
| format | Article |
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Its structural outlines are given and we show that: 1) a single point mutation causing a substitution of valine for glycine in codon 12 was found by DNA sequencing; 2) the c-ras-Ki gene is amplified and overexpressed in the original OHA tumor cells and its transformants and 3) the gene product is an abnormal form of the p21 protein.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(87)90542-0</identifier><identifier>PMID: 3476115</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier</publisher><subject>Amino Acid Sequence ; Animals ; Applied sciences ; Base Sequence ; Cell Line ; Cell Transformation, Neoplastic ; Cloning, Molecular ; Exact sciences and technology ; Gene Amplification ; Gene Expression Regulation ; Humans ; Mice ; Oncogenes ; Osteosarcoma - genetics ; Other techniques and industries ; Proto-Oncogenes</subject><ispartof>Biochemical and biophysical research communications, 1987-07, Vol.146 (2), p.395-402</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-8150309e83ff997258a883945f8b48d9bfcd2fc387d9ed1a065341dbcbfc2ede3</citedby><cites>FETCH-LOGICAL-c331t-8150309e83ff997258a883945f8b48d9bfcd2fc387d9ed1a065341dbcbfc2ede3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7844289$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3476115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NARDEUX, P. 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Its structural outlines are given and we show that: 1) a single point mutation causing a substitution of valine for glycine in codon 12 was found by DNA sequencing; 2) the c-ras-Ki gene is amplified and overexpressed in the original OHA tumor cells and its transformants and 3) the gene product is an abnormal form of the p21 protein.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Applied sciences</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cloning, Molecular</subject><subject>Exact sciences and technology</subject><subject>Gene Amplification</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Mice</subject><subject>Oncogenes</subject><subject>Osteosarcoma - genetics</subject><subject>Other techniques and industries</subject><subject>Proto-Oncogenes</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1LHDEUhkNRdLX9BxVyIVLBaU8mmUlyuSz1AwWhH9C7kE1ONDIzWZPZpf333dFlrw6c93kPh4eQzwy-MmDtNwBoq1qzP1-UvNTQiLqCD2TGQENVMxAHZLZHjslJKS8AjIlWH5EjLmTLWDMjZk4X1Y_5z-o-0jS49IQD0liodWPc2BH9FbX9qoshoqd28DRtMOPfVcZStps4UEuf170daCojpmKzS72lDruOdnHAj-Qw2K7gp908Jb-vv_9a3FYPjzd3i_lD5ThnY6VYAxw0Kh6C1rJulFWKa9EEtRTK62Vwvg6OK-k1emahbbhgfum2QY0e-Sm5eL-7yul1jWU0fSzTF3bAtC5GylYCCNiC4h10OZWSMZhVjr3N_wwDM3k1kzQzSTNKmjevZqqd7e6vlz36fWkncpuf73JbnO1CtoOLZY9JJUStNP8Pi6p_4w</recordid><startdate>19870731</startdate><enddate>19870731</enddate><creator>NARDEUX, P. 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Its structural outlines are given and we show that: 1) a single point mutation causing a substitution of valine for glycine in codon 12 was found by DNA sequencing; 2) the c-ras-Ki gene is amplified and overexpressed in the original OHA tumor cells and its transformants and 3) the gene product is an abnormal form of the p21 protein.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>3476115</pmid><doi>10.1016/0006-291X(87)90542-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1987-07, Vol.146 (2), p.395-402 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Applied sciences Base Sequence Cell Line Cell Transformation, Neoplastic Cloning, Molecular Exact sciences and technology Gene Amplification Gene Expression Regulation Humans Mice Oncogenes Osteosarcoma - genetics Other techniques and industries Proto-Oncogenes |
| title | A C-RAS-Ki oncogene is activated, amplified and overexpressed in a human osteosarcoma cell line |
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