Morphological changes in lymph nodes and expression of VCAM1 and cytokines at the late stages of SIV-induced disease in rhesus monkeys
Four patterns of structural alterations were found in lymph nodes (LNs) from rhesus monkeys 17 to 34 months after infection with simian immunodeficiency virus (SIVmac251). SIV p27 gag antigen and viral particles were localized either between the processes of follicular dendritic cells (FDCs) or in t...
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| Veröffentlicht in: | Research in virology (Paris) 1995-05, Vol.146 (3), p.185-200 |
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| creator | Persidsky, Y. Steffan, A.M. Gendrault, J.L. Royer, C. Beyer, C. Muchmore, E. Kirn, A. Aubertin, A.M. |
| description | Four patterns of structural alterations were found in lymph nodes (LNs) from rhesus monkeys 17 to 34 months after infection with simian immunodeficiency virus (SIVmac251). SIV p27
gag antigen and viral particles were localized either between the processes of follicular dendritic cells (FDCs) or in the cytoplasm of macrophages. In hyperplastic follicles, enlarged germinal centres contained numerous Ki67
+ proliferating centroblasts which were rather rare in light zones occupied by the CD23
+ FDC network. Involuted follicles contained a small number of Ki67
+ centroblasts and the CD23 labelling was limited to a very small apical zone. A correlation was found between the morphological characteristics of the follicles (hyperplasia-involution) and the level of expression of the vascular cell adhesion molecule 1 (VCAM1) on FDCs. A gradient in VCAM1 intensity with no expression in the subcapsular-intermediary sinuses, low membrane labelling in the mantle and strong expression in the FDC network was observed. IL1α
+ and IL6
+ (interleukin) cells (lymphocytes and macrophages) were detected in the mantle, the interfollicular area and the medulla of LNs. Expression of the tumour necrosis factor α and ultrastructural markers of interferon α production were found in a few FDC and macrophages. Our findings indicate a close relationship between the morphofunctional properties of FDC and the LN structure in SIV infection.
Quatre types d'altérations structurales ont été trouvés dans des ganglions lymphatiques de macaques entre 17 et 34 mois après infection par le virus de l'immunodéficience simienne (SIVmac251). L'antigène SIV
gagp27 ainsi que des particules virales ont été détectés entre les extensions cytoplasmiques des cellules folliculaires dendritiques (CFD) ainsi que dans le cytoplasme de macrophages. Dans des follicules hyperplasiques, les centres germinatifs dilatés renferment un grand nombre de centroblastes exprimant l'antigène Ki67, signe d'une intense activité proliférative. Ces cellules sont plutôt rares dans les zones claires occupées par le réseau des CFD CD23
+. Dans les follicules en involution, un faible nombre de centroblastes Ki67
+ a été détecté et le marquage du CD23 s'est limité à une surface très réduite de la zone apicale. Une corrélation entre les caractères morphologiques des follicules (hyperplasie-involution) et l'intensité de l'expression de la molécule d'adhérence vasculaire (VCAM1) par les CFD a pu être établie. Il existe un gradient dans l' |
| doi_str_mv | 10.1016/0923-2516(96)80579-6 |
| format | Article |
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gag antigen and viral particles were localized either between the processes of follicular dendritic cells (FDCs) or in the cytoplasm of macrophages. In hyperplastic follicles, enlarged germinal centres contained numerous Ki67
+ proliferating centroblasts which were rather rare in light zones occupied by the CD23
+ FDC network. Involuted follicles contained a small number of Ki67
+ centroblasts and the CD23 labelling was limited to a very small apical zone. A correlation was found between the morphological characteristics of the follicles (hyperplasia-involution) and the level of expression of the vascular cell adhesion molecule 1 (VCAM1) on FDCs. A gradient in VCAM1 intensity with no expression in the subcapsular-intermediary sinuses, low membrane labelling in the mantle and strong expression in the FDC network was observed. IL1α
+ and IL6
+ (interleukin) cells (lymphocytes and macrophages) were detected in the mantle, the interfollicular area and the medulla of LNs. Expression of the tumour necrosis factor α and ultrastructural markers of interferon α production were found in a few FDC and macrophages. Our findings indicate a close relationship between the morphofunctional properties of FDC and the LN structure in SIV infection.
Quatre types d'altérations structurales ont été trouvés dans des ganglions lymphatiques de macaques entre 17 et 34 mois après infection par le virus de l'immunodéficience simienne (SIVmac251). L'antigène SIV
gagp27 ainsi que des particules virales ont été détectés entre les extensions cytoplasmiques des cellules folliculaires dendritiques (CFD) ainsi que dans le cytoplasme de macrophages. Dans des follicules hyperplasiques, les centres germinatifs dilatés renferment un grand nombre de centroblastes exprimant l'antigène Ki67, signe d'une intense activité proliférative. Ces cellules sont plutôt rares dans les zones claires occupées par le réseau des CFD CD23
+. Dans les follicules en involution, un faible nombre de centroblastes Ki67
+ a été détecté et le marquage du CD23 s'est limité à une surface très réduite de la zone apicale. Une corrélation entre les caractères morphologiques des follicules (hyperplasie-involution) et l'intensité de l'expression de la molécule d'adhérence vasculaire (VCAM1) par les CFD a pu être établie. Il existe un gradient dans l'intensité de marquage de VCAM1 entre les sinus sous-capsulaires intermédiaires (absence d'expression), le cortex (faible marquage membranaire) et le réseau des CFD (marquage intense). Des lymphocytes et des macrophages expriment de I'ILlα et de l'IL6 (interleukine) dans le cortex et les régions interfolliculaire et médullaire. De rares CFD et macrophages expriment du TNFα (tumour necrosis factor) ou présentent des marqueurs ultrastructuraux associés à la production d'IFNα (interféron). Nos résultats démontrent une relation étroite entre les caractères morphologiques et les propriétés fonctionnelles des CFD et la structure des ganglions au cours de l'infection par le SIV.</description><identifier>ISSN: 0923-2516</identifier><identifier>DOI: 10.1016/0923-2516(96)80579-6</identifier><identifier>PMID: 7481091</identifier><language>eng</language><publisher>Paris: Elsevier B.V</publisher><subject>AIDS/HIV ; Animals ; Antigens, Viral - biosynthesis ; Biological and medical sciences ; Cell Line ; Cellule folliculaire dendritique ; Cytokines - biosynthesis ; Follicular dendritic cell ; Fundamental and applied biological sciences. Psychology ; Ganglion lymphatique ; Gene Products, gag - metabolism ; IFNα ; IL1 ; IL6 ; ILl ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Lymph node ; Lymph Nodes - immunology ; Lymph Nodes - ultrastructure ; Macaca mulatta ; Medical sciences ; Microbiology ; Morphofunctional properties ; Propriétés morphofonctionnelles ; Receptors, IgE - biosynthesis ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Simian Acquired Immunodeficiency Syndrome - immunology ; Simian Acquired Immunodeficiency Syndrome - pathology ; simian immunodeficiency virus ; Simian Immunodeficiency Virus - ultrastructure ; SIV ; TNFα ; Vascular Cell Adhesion Molecule-1 - biosynthesis ; VCAM1 ; Virology</subject><ispartof>Research in virology (Paris), 1995-05, Vol.146 (3), p.185-200</ispartof><rights>1995 INSTITUT PASTEUR/ELSEVIER</rights><rights>1995 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-63d76e7d7365b6bf17869fed28a3810d3ddcc82efebf40d2e95b49d21fad8d8d3</citedby><cites>FETCH-LOGICAL-c483t-63d76e7d7365b6bf17869fed28a3810d3ddcc82efebf40d2e95b49d21fad8d8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3586229$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7481091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Persidsky, Y.</creatorcontrib><creatorcontrib>Steffan, A.M.</creatorcontrib><creatorcontrib>Gendrault, J.L.</creatorcontrib><creatorcontrib>Royer, C.</creatorcontrib><creatorcontrib>Beyer, C.</creatorcontrib><creatorcontrib>Muchmore, E.</creatorcontrib><creatorcontrib>Kirn, A.</creatorcontrib><creatorcontrib>Aubertin, A.M.</creatorcontrib><title>Morphological changes in lymph nodes and expression of VCAM1 and cytokines at the late stages of SIV-induced disease in rhesus monkeys</title><title>Research in virology (Paris)</title><addtitle>Res Virol</addtitle><description>Four patterns of structural alterations were found in lymph nodes (LNs) from rhesus monkeys 17 to 34 months after infection with simian immunodeficiency virus (SIVmac251). SIV p27
gag antigen and viral particles were localized either between the processes of follicular dendritic cells (FDCs) or in the cytoplasm of macrophages. In hyperplastic follicles, enlarged germinal centres contained numerous Ki67
+ proliferating centroblasts which were rather rare in light zones occupied by the CD23
+ FDC network. Involuted follicles contained a small number of Ki67
+ centroblasts and the CD23 labelling was limited to a very small apical zone. A correlation was found between the morphological characteristics of the follicles (hyperplasia-involution) and the level of expression of the vascular cell adhesion molecule 1 (VCAM1) on FDCs. A gradient in VCAM1 intensity with no expression in the subcapsular-intermediary sinuses, low membrane labelling in the mantle and strong expression in the FDC network was observed. IL1α
+ and IL6
+ (interleukin) cells (lymphocytes and macrophages) were detected in the mantle, the interfollicular area and the medulla of LNs. Expression of the tumour necrosis factor α and ultrastructural markers of interferon α production were found in a few FDC and macrophages. Our findings indicate a close relationship between the morphofunctional properties of FDC and the LN structure in SIV infection.
Quatre types d'altérations structurales ont été trouvés dans des ganglions lymphatiques de macaques entre 17 et 34 mois après infection par le virus de l'immunodéficience simienne (SIVmac251). L'antigène SIV
gagp27 ainsi que des particules virales ont été détectés entre les extensions cytoplasmiques des cellules folliculaires dendritiques (CFD) ainsi que dans le cytoplasme de macrophages. Dans des follicules hyperplasiques, les centres germinatifs dilatés renferment un grand nombre de centroblastes exprimant l'antigène Ki67, signe d'une intense activité proliférative. Ces cellules sont plutôt rares dans les zones claires occupées par le réseau des CFD CD23
+. Dans les follicules en involution, un faible nombre de centroblastes Ki67
+ a été détecté et le marquage du CD23 s'est limité à une surface très réduite de la zone apicale. Une corrélation entre les caractères morphologiques des follicules (hyperplasie-involution) et l'intensité de l'expression de la molécule d'adhérence vasculaire (VCAM1) par les CFD a pu être établie. Il existe un gradient dans l'intensité de marquage de VCAM1 entre les sinus sous-capsulaires intermédiaires (absence d'expression), le cortex (faible marquage membranaire) et le réseau des CFD (marquage intense). Des lymphocytes et des macrophages expriment de I'ILlα et de l'IL6 (interleukine) dans le cortex et les régions interfolliculaire et médullaire. De rares CFD et macrophages expriment du TNFα (tumour necrosis factor) ou présentent des marqueurs ultrastructuraux associés à la production d'IFNα (interféron). Nos résultats démontrent une relation étroite entre les caractères morphologiques et les propriétés fonctionnelles des CFD et la structure des ganglions au cours de l'infection par le SIV.</description><subject>AIDS/HIV</subject><subject>Animals</subject><subject>Antigens, Viral - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cellule folliculaire dendritique</subject><subject>Cytokines - biosynthesis</subject><subject>Follicular dendritic cell</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglion lymphatique</subject><subject>Gene Products, gag - metabolism</subject><subject>IFNα</subject><subject>IL1</subject><subject>IL6</subject><subject>ILl</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Lymph node</subject><subject>Lymph Nodes - immunology</subject><subject>Lymph Nodes - ultrastructure</subject><subject>Macaca mulatta</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Morphofunctional properties</subject><subject>Propriétés morphofonctionnelles</subject><subject>Receptors, IgE - biosynthesis</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Simian Acquired Immunodeficiency Syndrome - immunology</subject><subject>Simian Acquired Immunodeficiency Syndrome - pathology</subject><subject>simian immunodeficiency virus</subject><subject>Simian Immunodeficiency Virus - ultrastructure</subject><subject>SIV</subject><subject>TNFα</subject><subject>Vascular Cell Adhesion Molecule-1 - biosynthesis</subject><subject>VCAM1</subject><subject>Virology</subject><issn>0923-2516</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhnMAlVJ4A5B8QKgcAraT2PEFqVpRqNSKA9Cr5diTxjRrB0-C2Bfoc-N0V3sE-WB55vMve76ieMXoe0aZ-EAVr0reMHGuxLuWNlKV4klxeiw_K54j_qSUyorLk-JE1i2jip0WDzcxTUMc4523ZiR2MOEOkPhAxt12GkiILh9NcAT-TAkQfQwk9uR2c3HDHut2N8d7H1ZqJvMAZDQzEJzNmpPJb1e3pQ9useCI8wgGYY1PA-CCZBvDPezwRfG0NyPCy8N-Vvy4_PR986W8_vr5anNxXdq6reZSVE4KkE5WoulE1zPZCtWD462p8odc5Zy1LYceur6mjoNqulo5znrj2ryqs-LtPndK8dcCOOutRwvjaALEBbWUQijeNP8FmWhrziXLYL0HbYqICXo9Jb81aacZ1asbvUrQqwSthH50o0W-9vqQv3RbcMdLBzG5_-bQN5jF9MkE6_GIVU0rOFcZ-7jHIA_tt4ek0XoIedY-gZ21i_7f7_gLOYmucQ</recordid><startdate>19950501</startdate><enddate>19950501</enddate><creator>Persidsky, Y.</creator><creator>Steffan, A.M.</creator><creator>Gendrault, J.L.</creator><creator>Royer, C.</creator><creator>Beyer, C.</creator><creator>Muchmore, E.</creator><creator>Kirn, A.</creator><creator>Aubertin, A.M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19950501</creationdate><title>Morphological changes in lymph nodes and expression of VCAM1 and cytokines at the late stages of SIV-induced disease in rhesus monkeys</title><author>Persidsky, Y. ; Steffan, A.M. ; Gendrault, J.L. ; Royer, C. ; Beyer, C. ; Muchmore, E. ; Kirn, A. ; Aubertin, A.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-63d76e7d7365b6bf17869fed28a3810d3ddcc82efebf40d2e95b49d21fad8d8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AIDS/HIV</topic><topic>Animals</topic><topic>Antigens, Viral - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cellule folliculaire dendritique</topic><topic>Cytokines - biosynthesis</topic><topic>Follicular dendritic cell</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ganglion lymphatique</topic><topic>Gene Products, gag - metabolism</topic><topic>IFNα</topic><topic>IL1</topic><topic>IL6</topic><topic>ILl</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Lymph node</topic><topic>Lymph Nodes - immunology</topic><topic>Lymph Nodes - ultrastructure</topic><topic>Macaca mulatta</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Morphofunctional properties</topic><topic>Propriétés morphofonctionnelles</topic><topic>Receptors, IgE - biosynthesis</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Simian Acquired Immunodeficiency Syndrome - immunology</topic><topic>Simian Acquired Immunodeficiency Syndrome - pathology</topic><topic>simian immunodeficiency virus</topic><topic>Simian Immunodeficiency Virus - ultrastructure</topic><topic>SIV</topic><topic>TNFα</topic><topic>Vascular Cell Adhesion Molecule-1 - biosynthesis</topic><topic>VCAM1</topic><topic>Virology</topic><toplevel>online_resources</toplevel><creatorcontrib>Persidsky, Y.</creatorcontrib><creatorcontrib>Steffan, A.M.</creatorcontrib><creatorcontrib>Gendrault, J.L.</creatorcontrib><creatorcontrib>Royer, C.</creatorcontrib><creatorcontrib>Beyer, C.</creatorcontrib><creatorcontrib>Muchmore, E.</creatorcontrib><creatorcontrib>Kirn, A.</creatorcontrib><creatorcontrib>Aubertin, A.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in virology (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Persidsky, Y.</au><au>Steffan, A.M.</au><au>Gendrault, J.L.</au><au>Royer, C.</au><au>Beyer, C.</au><au>Muchmore, E.</au><au>Kirn, A.</au><au>Aubertin, A.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological changes in lymph nodes and expression of VCAM1 and cytokines at the late stages of SIV-induced disease in rhesus monkeys</atitle><jtitle>Research in virology (Paris)</jtitle><addtitle>Res Virol</addtitle><date>1995-05-01</date><risdate>1995</risdate><volume>146</volume><issue>3</issue><spage>185</spage><epage>200</epage><pages>185-200</pages><issn>0923-2516</issn><abstract>Four patterns of structural alterations were found in lymph nodes (LNs) from rhesus monkeys 17 to 34 months after infection with simian immunodeficiency virus (SIVmac251). SIV p27
gag antigen and viral particles were localized either between the processes of follicular dendritic cells (FDCs) or in the cytoplasm of macrophages. In hyperplastic follicles, enlarged germinal centres contained numerous Ki67
+ proliferating centroblasts which were rather rare in light zones occupied by the CD23
+ FDC network. Involuted follicles contained a small number of Ki67
+ centroblasts and the CD23 labelling was limited to a very small apical zone. A correlation was found between the morphological characteristics of the follicles (hyperplasia-involution) and the level of expression of the vascular cell adhesion molecule 1 (VCAM1) on FDCs. A gradient in VCAM1 intensity with no expression in the subcapsular-intermediary sinuses, low membrane labelling in the mantle and strong expression in the FDC network was observed. IL1α
+ and IL6
+ (interleukin) cells (lymphocytes and macrophages) were detected in the mantle, the interfollicular area and the medulla of LNs. Expression of the tumour necrosis factor α and ultrastructural markers of interferon α production were found in a few FDC and macrophages. Our findings indicate a close relationship between the morphofunctional properties of FDC and the LN structure in SIV infection.
Quatre types d'altérations structurales ont été trouvés dans des ganglions lymphatiques de macaques entre 17 et 34 mois après infection par le virus de l'immunodéficience simienne (SIVmac251). L'antigène SIV
gagp27 ainsi que des particules virales ont été détectés entre les extensions cytoplasmiques des cellules folliculaires dendritiques (CFD) ainsi que dans le cytoplasme de macrophages. Dans des follicules hyperplasiques, les centres germinatifs dilatés renferment un grand nombre de centroblastes exprimant l'antigène Ki67, signe d'une intense activité proliférative. Ces cellules sont plutôt rares dans les zones claires occupées par le réseau des CFD CD23
+. Dans les follicules en involution, un faible nombre de centroblastes Ki67
+ a été détecté et le marquage du CD23 s'est limité à une surface très réduite de la zone apicale. Une corrélation entre les caractères morphologiques des follicules (hyperplasie-involution) et l'intensité de l'expression de la molécule d'adhérence vasculaire (VCAM1) par les CFD a pu être établie. Il existe un gradient dans l'intensité de marquage de VCAM1 entre les sinus sous-capsulaires intermédiaires (absence d'expression), le cortex (faible marquage membranaire) et le réseau des CFD (marquage intense). Des lymphocytes et des macrophages expriment de I'ILlα et de l'IL6 (interleukine) dans le cortex et les régions interfolliculaire et médullaire. De rares CFD et macrophages expriment du TNFα (tumour necrosis factor) ou présentent des marqueurs ultrastructuraux associés à la production d'IFNα (interféron). Nos résultats démontrent une relation étroite entre les caractères morphologiques et les propriétés fonctionnelles des CFD et la structure des ganglions au cours de l'infection par le SIV.</abstract><cop>Paris</cop><pub>Elsevier B.V</pub><pmid>7481091</pmid><doi>10.1016/0923-2516(96)80579-6</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0923-2516 |
| ispartof | Research in virology (Paris), 1995-05, Vol.146 (3), p.185-200 |
| issn | 0923-2516 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77669255 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | AIDS/HIV Animals Antigens, Viral - biosynthesis Biological and medical sciences Cell Line Cellule folliculaire dendritique Cytokines - biosynthesis Follicular dendritic cell Fundamental and applied biological sciences. Psychology Ganglion lymphatique Gene Products, gag - metabolism IFNα IL1 IL6 ILl Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Lymph node Lymph Nodes - immunology Lymph Nodes - ultrastructure Macaca mulatta Medical sciences Microbiology Morphofunctional properties Propriétés morphofonctionnelles Receptors, IgE - biosynthesis Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Simian Acquired Immunodeficiency Syndrome - immunology Simian Acquired Immunodeficiency Syndrome - pathology simian immunodeficiency virus Simian Immunodeficiency Virus - ultrastructure SIV TNFα Vascular Cell Adhesion Molecule-1 - biosynthesis VCAM1 Virology |
| title | Morphological changes in lymph nodes and expression of VCAM1 and cytokines at the late stages of SIV-induced disease in rhesus monkeys |
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