Spectrophotometric determination of hemoglobin pigments in neonatal blood
Fetal and adult hemoglobin pigments have slightly different light absorption coefficients. Blood from newborns therefore gives inaccurate results with a direct spectrophotometric determination of hemoglobin pigments (Radiometer's OSM3), if the absorption coefficients for adult blood are used. W...
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| Veröffentlicht in: | Clinica chimica acta 1987-07, Vol.166 (2), p.291-296 |
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| container_title | Clinica chimica acta |
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| creator | Fogh-Andersen, Niels Siggaard-Andersen, Ole Lundsgaard, Finn C. Wimberley, Peter D. |
| description | Fetal and adult hemoglobin pigments have slightly different light absorption coefficients. Blood from newborns therefore gives inaccurate results with a direct spectrophotometric determination of hemoglobin pigments (Radiometer's OSM3), if the absorption coefficients for adult blood are used. We determined an absorption coefficient matrix for hemoglobin pigments in twenty full term newborns' blood. This matrix yielded results accurate to within 0.2% in 40 further newborns, but the accuracy of the results varied with the individual ratio of fetal to total hemoglobin, which was 80 ± 5% (
sd) in the examined samples. The OSM3's inaccuracy with the adult absorption coefficients can be used to directly estimate the ratio of fetal to total hemoglobin in an infant. |
| doi_str_mv | 10.1016/0009-8981(87)90432-3 |
| format | Article |
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sd) in the examined samples. The OSM3's inaccuracy with the adult absorption coefficients can be used to directly estimate the ratio of fetal to total hemoglobin in an infant.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(87)90432-3</identifier><identifier>PMID: 3621606</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Adult ; Biological and medical sciences ; Carboxyhemoglobin, Fetal hemoglobin, Methemoglobin ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fetal Blood - analysis ; Fundamental and applied biological sciences. Psychology ; Hemoglobins - analysis ; Humans ; Infant, Newborn - blood ; Male ; Organogenesis. Fetal development ; Organogenesis. Physiological fonctions ; Oxygen saturation ; Reference Values ; Spectrophotometry - instrumentation ; Sulfhemoglobin</subject><ispartof>Clinica chimica acta, 1987-07, Vol.166 (2), p.291-296</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-de4e1ae5868394d5db55fa6a2e9c34586f6f02d70674ded7aa8fd3694c32e3243</citedby><cites>FETCH-LOGICAL-c386t-de4e1ae5868394d5db55fa6a2e9c34586f6f02d70674ded7aa8fd3694c32e3243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0009-8981(87)90432-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7520797$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3621606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fogh-Andersen, Niels</creatorcontrib><creatorcontrib>Siggaard-Andersen, Ole</creatorcontrib><creatorcontrib>Lundsgaard, Finn C.</creatorcontrib><creatorcontrib>Wimberley, Peter D.</creatorcontrib><title>Spectrophotometric determination of hemoglobin pigments in neonatal blood</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Fetal and adult hemoglobin pigments have slightly different light absorption coefficients. Blood from newborns therefore gives inaccurate results with a direct spectrophotometric determination of hemoglobin pigments (Radiometer's OSM3), if the absorption coefficients for adult blood are used. We determined an absorption coefficient matrix for hemoglobin pigments in twenty full term newborns' blood. This matrix yielded results accurate to within 0.2% in 40 further newborns, but the accuracy of the results varied with the individual ratio of fetal to total hemoglobin, which was 80 ± 5% (
sd) in the examined samples. The OSM3's inaccuracy with the adult absorption coefficients can be used to directly estimate the ratio of fetal to total hemoglobin in an infant.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Carboxyhemoglobin, Fetal hemoglobin, Methemoglobin</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fetal Blood - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemoglobins - analysis</subject><subject>Humans</subject><subject>Infant, Newborn - blood</subject><subject>Male</subject><subject>Organogenesis. Fetal development</subject><subject>Organogenesis. Physiological fonctions</subject><subject>Oxygen saturation</subject><subject>Reference Values</subject><subject>Spectrophotometry - instrumentation</subject><subject>Sulfhemoglobin</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEQgIMotVb_gcIeRPSwmmyySfYiSPFRKHhQzyFNZtvI7mZNtoL_3tSWHj3N65th-BA6J_iWYMLvMMZVLitJrqW4qTCjRU4P0JhIQXPKquIQjffIMTqJ8TOVDHMyQiPKC8IxH6PZWw9mCL5f-cG3MARnMgsDhNZ1enC-y3ydraD1y8YvXJf1btlCN8Qs5R34xOgmWzTe21N0VOsmwtkuTtDH0-P79CWfvz7Ppg_z3FDJh9wCA6KhlFzSitnSLsqy1lwXUBnKUrvmNS6swFwwC1ZoLWtLecUMLYAWjE7Q1fZuH_zXGuKgWhcNNI1O_6yjEoJzWXKaQLYFTfAxBqhVH1yrw48iWG0Mqo0etdGjpFB_BtVm7WJ3f71owe6XdsrS_HI319Hopg66My7uMVEWWFQiYfdbDJKLbwdBReOgM2BdSMaV9e7_P34BkJWNcw</recordid><startdate>19870715</startdate><enddate>19870715</enddate><creator>Fogh-Andersen, Niels</creator><creator>Siggaard-Andersen, Ole</creator><creator>Lundsgaard, Finn C.</creator><creator>Wimberley, Peter D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870715</creationdate><title>Spectrophotometric determination of hemoglobin pigments in neonatal blood</title><author>Fogh-Andersen, Niels ; Siggaard-Andersen, Ole ; Lundsgaard, Finn C. ; Wimberley, Peter D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-de4e1ae5868394d5db55fa6a2e9c34586f6f02d70674ded7aa8fd3694c32e3243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Carboxyhemoglobin, Fetal hemoglobin, Methemoglobin</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fetal Blood - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemoglobins - analysis</topic><topic>Humans</topic><topic>Infant, Newborn - blood</topic><topic>Male</topic><topic>Organogenesis. Fetal development</topic><topic>Organogenesis. Physiological fonctions</topic><topic>Oxygen saturation</topic><topic>Reference Values</topic><topic>Spectrophotometry - instrumentation</topic><topic>Sulfhemoglobin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fogh-Andersen, Niels</creatorcontrib><creatorcontrib>Siggaard-Andersen, Ole</creatorcontrib><creatorcontrib>Lundsgaard, Finn C.</creatorcontrib><creatorcontrib>Wimberley, Peter D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fogh-Andersen, Niels</au><au>Siggaard-Andersen, Ole</au><au>Lundsgaard, Finn C.</au><au>Wimberley, Peter D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spectrophotometric determination of hemoglobin pigments in neonatal blood</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1987-07-15</date><risdate>1987</risdate><volume>166</volume><issue>2</issue><spage>291</spage><epage>296</epage><pages>291-296</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>Fetal and adult hemoglobin pigments have slightly different light absorption coefficients. Blood from newborns therefore gives inaccurate results with a direct spectrophotometric determination of hemoglobin pigments (Radiometer's OSM3), if the absorption coefficients for adult blood are used. We determined an absorption coefficient matrix for hemoglobin pigments in twenty full term newborns' blood. This matrix yielded results accurate to within 0.2% in 40 further newborns, but the accuracy of the results varied with the individual ratio of fetal to total hemoglobin, which was 80 ± 5% (
sd) in the examined samples. The OSM3's inaccuracy with the adult absorption coefficients can be used to directly estimate the ratio of fetal to total hemoglobin in an infant.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>3621606</pmid><doi>10.1016/0009-8981(87)90432-3</doi><tpages>6</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1987-07, Vol.166 (2), p.291-296 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Biological and medical sciences Carboxyhemoglobin, Fetal hemoglobin, Methemoglobin Embryology: invertebrates and vertebrates. Teratology Female Fetal Blood - analysis Fundamental and applied biological sciences. Psychology Hemoglobins - analysis Humans Infant, Newborn - blood Male Organogenesis. Fetal development Organogenesis. Physiological fonctions Oxygen saturation Reference Values Spectrophotometry - instrumentation Sulfhemoglobin |
| title | Spectrophotometric determination of hemoglobin pigments in neonatal blood |
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