Breath pentane analysis as an index of lipid peroxidation: a functional test of vitamin E status
Pentane, which evolves from the reaction involving omega-6 fatty acids, is a good index of lipid peroxidation. We describe a method for measuring breath pentane excretion in adult humans. After a 4-minute washout period, expired air was analyzed by gas chromatography. Breath was passed through a coo...
Gespeichert in:
| Veröffentlicht in: | The American journal of clinical nutrition 1987-08, Vol.46 (2), p.267-272 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 272 |
|---|---|
| container_issue | 2 |
| container_start_page | 267 |
| container_title | The American journal of clinical nutrition |
| container_volume | 46 |
| creator | Lemoyne, M Van Gossum, A Kurian, R Ostro, M Axler, J Jeejeebhoy, K N |
| description | Pentane, which evolves from the reaction involving omega-6 fatty acids, is a good index of lipid peroxidation. We describe a method for measuring breath pentane excretion in adult humans. After a 4-minute washout period, expired air was analyzed by gas chromatography. Breath was passed through a cooled loop of alumina to adsorb, concentrate, and release, on heating, pentane. Pentane was analyzed by a Porasil-D column with a derived calibration curve. The mean excretion of pentane in 10 normal adults was 6.34 +/- 0.96 pmol X kg-1 X min-1 (mean +/- SEM) and was significantly higher in five patients with plasma vitamin E deficiency (15.39 +/- 1.84 pmol X kg-1 X min-1). There was a significant negative correlation between pentane output and plasma vitamin E levels (r = -0.66, p less than 0.01). Moreover, breath pentane excretion was significantly decreased after a 10-d supplementation with vitamin E in five normal subjects. We conclude that breath pentane output is a sensitive, noninvasive, functional test for assessing vitamin E status. |
| doi_str_mv | 10.1093/ajcn/46.2.267 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77667720</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S000291652340322X</els_id><sourcerecordid>77667720</sourcerecordid><originalsourceid>FETCH-LOGICAL-c425t-e2378c03a7431edda7c05c3b3808c678f76f8d2dd72765121da9a1b7cf5505f23</originalsourceid><addsrcrecordid>eNp1kMtLXDEUxkNp0al22Z2FLKS7O-Zx85juVOwDBBfqOj2Th0bu5E6TXNH_3lxmcFcInMD34zvf-RD6SsmSkhU_gyebznq5ZEsm1Qe0oCuuO86I-ogWhBDWragUh-hzKU-EUNZreYAOOKWcMb1Afy-yh_qItz5VSB5DguG1xIKhvYRjcv4FjwEPcRtdo_L4Eh3UOKYfGHCYkp3_MODqS53B51hhExO-wqVCncox-hRgKP7Lfh6h-59Xd5e_u-ubX38uz6872zNRO8-40pZwUD2n3jlQlgjL11wTbaXSQcmgHXNOMSUFZdTBCuha2SAEEYHxI_R957vN47-phTGbWKwfhnbVOBWjlJRKMdLAbgfaPJaSfTDbHDeQXw0lZm7UzI2aXhpmWqON_7Y3ntYb797pfYVNP93rUCwMIUOysbxjbScTes53ssMCjAYeckPub7XmTDR5dlE72beOnqPPptjok_UuZm-rcWP8T743fZuaTQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77667720</pqid></control><display><type>article</type><title>Breath pentane analysis as an index of lipid peroxidation: a functional test of vitamin E status</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Lemoyne, M ; Van Gossum, A ; Kurian, R ; Ostro, M ; Axler, J ; Jeejeebhoy, K N</creator><creatorcontrib>Lemoyne, M ; Van Gossum, A ; Kurian, R ; Ostro, M ; Axler, J ; Jeejeebhoy, K N</creatorcontrib><description>Pentane, which evolves from the reaction involving omega-6 fatty acids, is a good index of lipid peroxidation. We describe a method for measuring breath pentane excretion in adult humans. After a 4-minute washout period, expired air was analyzed by gas chromatography. Breath was passed through a cooled loop of alumina to adsorb, concentrate, and release, on heating, pentane. Pentane was analyzed by a Porasil-D column with a derived calibration curve. The mean excretion of pentane in 10 normal adults was 6.34 +/- 0.96 pmol X kg-1 X min-1 (mean +/- SEM) and was significantly higher in five patients with plasma vitamin E deficiency (15.39 +/- 1.84 pmol X kg-1 X min-1). There was a significant negative correlation between pentane output and plasma vitamin E levels (r = -0.66, p less than 0.01). Moreover, breath pentane excretion was significantly decreased after a 10-d supplementation with vitamin E in five normal subjects. We conclude that breath pentane output is a sensitive, noninvasive, functional test for assessing vitamin E status.</description><identifier>ISSN: 0002-9165</identifier><identifier>EISSN: 1938-3207</identifier><identifier>DOI: 10.1093/ajcn/46.2.267</identifier><identifier>PMID: 3113228</identifier><identifier>CODEN: AJCNAC</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Adult ; ASETAT NUTRITIONNEL ; Biological and medical sciences ; Breath Tests ; Chromatography, Gas ; Enzymes. Coenzymes. Vitamins. Pigments ; Female ; Fundamental and applied biological sciences. Psychology ; HIDROCARB ; Home Care Services ; Humans ; HYDROCAR ; Lipid Peroxides - metabolism ; LIPIDE ; LIPIDOS ; Male ; Metabolisms and neurohumoral controls ; Middle Aged ; NUTRICION ; NUTRITION ; OXIDACION ; OXYDATION ; Parenteral Nutrition, Total - adverse effects ; Pentanes - analysis ; r)ESTADO NUTRICIONAL ; RESPIRACION ; RESPIRATION ; TOCOFEROLES ; TOCOPHEROL ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Vitamin E - metabolism ; Vitamin E Deficiency - diagnosis ; Vitamin E Deficiency - etiology</subject><ispartof>The American journal of clinical nutrition, 1987-08, Vol.46 (2), p.267-272</ispartof><rights>1987 American Society for Nutrition.</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-e2378c03a7431edda7c05c3b3808c678f76f8d2dd72765121da9a1b7cf5505f23</citedby><cites>FETCH-LOGICAL-c425t-e2378c03a7431edda7c05c3b3808c678f76f8d2dd72765121da9a1b7cf5505f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7722582$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3113228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lemoyne, M</creatorcontrib><creatorcontrib>Van Gossum, A</creatorcontrib><creatorcontrib>Kurian, R</creatorcontrib><creatorcontrib>Ostro, M</creatorcontrib><creatorcontrib>Axler, J</creatorcontrib><creatorcontrib>Jeejeebhoy, K N</creatorcontrib><title>Breath pentane analysis as an index of lipid peroxidation: a functional test of vitamin E status</title><title>The American journal of clinical nutrition</title><addtitle>Am J Clin Nutr</addtitle><description>Pentane, which evolves from the reaction involving omega-6 fatty acids, is a good index of lipid peroxidation. We describe a method for measuring breath pentane excretion in adult humans. After a 4-minute washout period, expired air was analyzed by gas chromatography. Breath was passed through a cooled loop of alumina to adsorb, concentrate, and release, on heating, pentane. Pentane was analyzed by a Porasil-D column with a derived calibration curve. The mean excretion of pentane in 10 normal adults was 6.34 +/- 0.96 pmol X kg-1 X min-1 (mean +/- SEM) and was significantly higher in five patients with plasma vitamin E deficiency (15.39 +/- 1.84 pmol X kg-1 X min-1). There was a significant negative correlation between pentane output and plasma vitamin E levels (r = -0.66, p less than 0.01). Moreover, breath pentane excretion was significantly decreased after a 10-d supplementation with vitamin E in five normal subjects. We conclude that breath pentane output is a sensitive, noninvasive, functional test for assessing vitamin E status.</description><subject>Adult</subject><subject>ASETAT NUTRITIONNEL</subject><subject>Biological and medical sciences</subject><subject>Breath Tests</subject><subject>Chromatography, Gas</subject><subject>Enzymes. Coenzymes. Vitamins. Pigments</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIDROCARB</subject><subject>Home Care Services</subject><subject>Humans</subject><subject>HYDROCAR</subject><subject>Lipid Peroxides - metabolism</subject><subject>LIPIDE</subject><subject>LIPIDOS</subject><subject>Male</subject><subject>Metabolisms and neurohumoral controls</subject><subject>Middle Aged</subject><subject>NUTRICION</subject><subject>NUTRITION</subject><subject>OXIDACION</subject><subject>OXYDATION</subject><subject>Parenteral Nutrition, Total - adverse effects</subject><subject>Pentanes - analysis</subject><subject>r)ESTADO NUTRICIONAL</subject><subject>RESPIRACION</subject><subject>RESPIRATION</subject><subject>TOCOFEROLES</subject><subject>TOCOPHEROL</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Vitamin E - metabolism</subject><subject>Vitamin E Deficiency - diagnosis</subject><subject>Vitamin E Deficiency - etiology</subject><issn>0002-9165</issn><issn>1938-3207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtLXDEUxkNp0al22Z2FLKS7O-Zx85juVOwDBBfqOj2Th0bu5E6TXNH_3lxmcFcInMD34zvf-RD6SsmSkhU_gyebznq5ZEsm1Qe0oCuuO86I-ogWhBDWragUh-hzKU-EUNZreYAOOKWcMb1Afy-yh_qItz5VSB5DguG1xIKhvYRjcv4FjwEPcRtdo_L4Eh3UOKYfGHCYkp3_MODqS53B51hhExO-wqVCncox-hRgKP7Lfh6h-59Xd5e_u-ubX38uz6872zNRO8-40pZwUD2n3jlQlgjL11wTbaXSQcmgHXNOMSUFZdTBCuha2SAEEYHxI_R957vN47-phTGbWKwfhnbVOBWjlJRKMdLAbgfaPJaSfTDbHDeQXw0lZm7UzI2aXhpmWqON_7Y3ntYb797pfYVNP93rUCwMIUOysbxjbScTes53ssMCjAYeckPub7XmTDR5dlE72beOnqPPptjok_UuZm-rcWP8T743fZuaTQ</recordid><startdate>19870801</startdate><enddate>19870801</enddate><creator>Lemoyne, M</creator><creator>Van Gossum, A</creator><creator>Kurian, R</creator><creator>Ostro, M</creator><creator>Axler, J</creator><creator>Jeejeebhoy, K N</creator><general>Elsevier Inc</general><general>American Society for Clinical Nutrition</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870801</creationdate><title>Breath pentane analysis as an index of lipid peroxidation: a functional test of vitamin E status</title><author>Lemoyne, M ; Van Gossum, A ; Kurian, R ; Ostro, M ; Axler, J ; Jeejeebhoy, K N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-e2378c03a7431edda7c05c3b3808c678f76f8d2dd72765121da9a1b7cf5505f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Adult</topic><topic>ASETAT NUTRITIONNEL</topic><topic>Biological and medical sciences</topic><topic>Breath Tests</topic><topic>Chromatography, Gas</topic><topic>Enzymes. Coenzymes. Vitamins. Pigments</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIDROCARB</topic><topic>Home Care Services</topic><topic>Humans</topic><topic>HYDROCAR</topic><topic>Lipid Peroxides - metabolism</topic><topic>LIPIDE</topic><topic>LIPIDOS</topic><topic>Male</topic><topic>Metabolisms and neurohumoral controls</topic><topic>Middle Aged</topic><topic>NUTRICION</topic><topic>NUTRITION</topic><topic>OXIDACION</topic><topic>OXYDATION</topic><topic>Parenteral Nutrition, Total - adverse effects</topic><topic>Pentanes - analysis</topic><topic>r)ESTADO NUTRICIONAL</topic><topic>RESPIRACION</topic><topic>RESPIRATION</topic><topic>TOCOFEROLES</topic><topic>TOCOPHEROL</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Vitamin E - metabolism</topic><topic>Vitamin E Deficiency - diagnosis</topic><topic>Vitamin E Deficiency - etiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lemoyne, M</creatorcontrib><creatorcontrib>Van Gossum, A</creatorcontrib><creatorcontrib>Kurian, R</creatorcontrib><creatorcontrib>Ostro, M</creatorcontrib><creatorcontrib>Axler, J</creatorcontrib><creatorcontrib>Jeejeebhoy, K N</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of clinical nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lemoyne, M</au><au>Van Gossum, A</au><au>Kurian, R</au><au>Ostro, M</au><au>Axler, J</au><au>Jeejeebhoy, K N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Breath pentane analysis as an index of lipid peroxidation: a functional test of vitamin E status</atitle><jtitle>The American journal of clinical nutrition</jtitle><addtitle>Am J Clin Nutr</addtitle><date>1987-08-01</date><risdate>1987</risdate><volume>46</volume><issue>2</issue><spage>267</spage><epage>272</epage><pages>267-272</pages><issn>0002-9165</issn><eissn>1938-3207</eissn><coden>AJCNAC</coden><abstract>Pentane, which evolves from the reaction involving omega-6 fatty acids, is a good index of lipid peroxidation. We describe a method for measuring breath pentane excretion in adult humans. After a 4-minute washout period, expired air was analyzed by gas chromatography. Breath was passed through a cooled loop of alumina to adsorb, concentrate, and release, on heating, pentane. Pentane was analyzed by a Porasil-D column with a derived calibration curve. The mean excretion of pentane in 10 normal adults was 6.34 +/- 0.96 pmol X kg-1 X min-1 (mean +/- SEM) and was significantly higher in five patients with plasma vitamin E deficiency (15.39 +/- 1.84 pmol X kg-1 X min-1). There was a significant negative correlation between pentane output and plasma vitamin E levels (r = -0.66, p less than 0.01). Moreover, breath pentane excretion was significantly decreased after a 10-d supplementation with vitamin E in five normal subjects. We conclude that breath pentane output is a sensitive, noninvasive, functional test for assessing vitamin E status.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>3113228</pmid><doi>10.1093/ajcn/46.2.267</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9165 |
| ispartof | The American journal of clinical nutrition, 1987-08, Vol.46 (2), p.267-272 |
| issn | 0002-9165 1938-3207 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77667720 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult ASETAT NUTRITIONNEL Biological and medical sciences Breath Tests Chromatography, Gas Enzymes. Coenzymes. Vitamins. Pigments Female Fundamental and applied biological sciences. Psychology HIDROCARB Home Care Services Humans HYDROCAR Lipid Peroxides - metabolism LIPIDE LIPIDOS Male Metabolisms and neurohumoral controls Middle Aged NUTRICION NUTRITION OXIDACION OXYDATION Parenteral Nutrition, Total - adverse effects Pentanes - analysis r)ESTADO NUTRICIONAL RESPIRACION RESPIRATION TOCOFEROLES TOCOPHEROL Vertebrates: anatomy and physiology, studies on body, several organs or systems Vitamin E - metabolism Vitamin E Deficiency - diagnosis Vitamin E Deficiency - etiology |
| title | Breath pentane analysis as an index of lipid peroxidation: a functional test of vitamin E status |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T10%3A00%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Breath%20pentane%20analysis%20as%20an%20index%20of%20lipid%20peroxidation:%20a%20functional%20test%20of%20vitamin%20E%20status&rft.jtitle=The%20American%20journal%20of%20clinical%20nutrition&rft.au=Lemoyne,%20M&rft.date=1987-08-01&rft.volume=46&rft.issue=2&rft.spage=267&rft.epage=272&rft.pages=267-272&rft.issn=0002-9165&rft.eissn=1938-3207&rft.coden=AJCNAC&rft_id=info:doi/10.1093/ajcn/46.2.267&rft_dat=%3Cproquest_cross%3E77667720%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77667720&rft_id=info:pmid/3113228&rft_els_id=S000291652340322X&rfr_iscdi=true |