Biosynthesis of reovirus-specified polypeptides Efficiency of expression of cDNAs of the reovirus S1 and S4 genes in transfected animal cells differs at the level of translation
Full-length cDNAs of the reovirus serotype 1 Lang strain S1 and S4 genes were cloned in Escherichia coli using bacteriophage M13 and expressed in monkey COS cells under the control of the SV40 late promoter using the eukaryotic expression vector pJC119. The s1-encoded σ1 and s4-encoded σ3 gene produ...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1987-08, Vol.159 (2), p.350-357 |
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| creator | Atwater, Jonathan A. Munemitsu, Susan M. Samuel, Charles E. |
| description | Full-length cDNAs of the reovirus serotype 1 Lang strain S1 and S4 genes were cloned in
Escherichia coli using bacteriophage M13 and expressed in monkey COS cells under the control of the SV40 late promoter using the eukaryotic expression vector pJC119. The
s1-encoded σ1 and
s4-encoded σ3 gene products were expressed in transfected COS cells and were indistinguishable from the authentic σ1 and σ3 polypeptides synthesized in reovirion-infected COS cells. The relative translational efficiencies of the
s1 and
s4 mRNAs in transfected COS cells were similar to the efficiencies observed in virion-infected cells; the
s4 mRNA was translated approximately five times more efficiently than the
s1 mRNA. Our results suggest that the differential translation of the reovirus
s1 and
s4 mRNAs
in vivo may be attributed to intrinsic structural properties of the individual mRNAs and is independent of competition with other viral mRNAs. |
| doi_str_mv | 10.1016/0042-6822(87)90473-9 |
| format | Article |
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Escherichia coli using bacteriophage M13 and expressed in monkey COS cells under the control of the SV40 late promoter using the eukaryotic expression vector pJC119. The
s1-encoded σ1 and
s4-encoded σ3 gene products were expressed in transfected COS cells and were indistinguishable from the authentic σ1 and σ3 polypeptides synthesized in reovirion-infected COS cells. The relative translational efficiencies of the
s1 and
s4 mRNAs in transfected COS cells were similar to the efficiencies observed in virion-infected cells; the
s4 mRNA was translated approximately five times more efficiently than the
s1 mRNA. Our results suggest that the differential translation of the reovirus
s1 and
s4 mRNAs
in vivo may be attributed to intrinsic structural properties of the individual mRNAs and is independent of competition with other viral mRNAs.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(87)90473-9</identifier><identifier>PMID: 3617502</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Line ; Cercopithecus aethiops ; DNA - genetics ; DNA, Recombinant ; DNA, Viral - genetics ; Genes, Viral ; Kidney ; Peptide Biosynthesis ; Plasmids ; Protein Biosynthesis ; Recombinant Fusion Proteins - biosynthesis ; Reoviridae - metabolism ; reovirus ; RNA, Messenger - genetics ; RNA, Viral - genetics ; Transfection ; Viral Proteins - biosynthesis</subject><ispartof>Virology (New York, N.Y.), 1987-08, Vol.159 (2), p.350-357</ispartof><rights>1987</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-4d6795de00a729f9c70abe4e1a7aa6d1ea15d89e485fc70fa86560fc67ade47d3</citedby><cites>FETCH-LOGICAL-c303t-4d6795de00a729f9c70abe4e1a7aa6d1ea15d89e485fc70fa86560fc67ade47d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(87)90473-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3617502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Atwater, Jonathan A.</creatorcontrib><creatorcontrib>Munemitsu, Susan M.</creatorcontrib><creatorcontrib>Samuel, Charles E.</creatorcontrib><title>Biosynthesis of reovirus-specified polypeptides Efficiency of expression of cDNAs of the reovirus S1 and S4 genes in transfected animal cells differs at the level of translation</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Full-length cDNAs of the reovirus serotype 1 Lang strain S1 and S4 genes were cloned in
Escherichia coli using bacteriophage M13 and expressed in monkey COS cells under the control of the SV40 late promoter using the eukaryotic expression vector pJC119. The
s1-encoded σ1 and
s4-encoded σ3 gene products were expressed in transfected COS cells and were indistinguishable from the authentic σ1 and σ3 polypeptides synthesized in reovirion-infected COS cells. The relative translational efficiencies of the
s1 and
s4 mRNAs in transfected COS cells were similar to the efficiencies observed in virion-infected cells; the
s4 mRNA was translated approximately five times more efficiently than the
s1 mRNA. Our results suggest that the differential translation of the reovirus
s1 and
s4 mRNAs
in vivo may be attributed to intrinsic structural properties of the individual mRNAs and is independent of competition with other viral mRNAs.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Cercopithecus aethiops</subject><subject>DNA - genetics</subject><subject>DNA, Recombinant</subject><subject>DNA, Viral - genetics</subject><subject>Genes, Viral</subject><subject>Kidney</subject><subject>Peptide Biosynthesis</subject><subject>Plasmids</subject><subject>Protein Biosynthesis</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Reoviridae - metabolism</subject><subject>reovirus</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Viral - genetics</subject><subject>Transfection</subject><subject>Viral Proteins - biosynthesis</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi1EVbaFNwDJJ0QPATtx7OSCVNoClSo4FM6Wa4_BKGsHT3bVfSzeECe72iOcrNH_zzfj-Ql5ydlbzrh8x5ioK9nV9ZtOXfRMqKbqn5AVZ72sWCP4U7I6Wp6RM8RfrNRKsVNy2kiuWlavyJ8PIeEuTj8BA9LkaYa0DXmDFY5ggw_g6JiG3QjjFBwgvfE-2ADR7mY3PI4ZEEOKc2Wvv1wukII7gug9pyY6ei_oD4iFECKdsonowU6FbmJYm4FaGAakLngPGamZFsYAWxgW4NwwmKkMek5OvBkQXhzec_L94823q8_V3ddPt1eXd5VtWDNVwknVtw4YM6rufW8VMw8ggBtljHQcDG9d14PoWl80bzrZSuatVMaBUK45J6_33DGn3xvASa8DzluaCGmDWikpZVt3_zVy0TdSKV6MYm-0OSFm8HrM5e95pznTc6R6zkvPeelO6SVS3Ze2Vwf-5mEN7th0yLDo7_c6lGtsA2SNS0DgQi4n1i6Ffw_4C5iBtA0</recordid><startdate>198708</startdate><enddate>198708</enddate><creator>Atwater, Jonathan A.</creator><creator>Munemitsu, Susan M.</creator><creator>Samuel, Charles E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198708</creationdate><title>Biosynthesis of reovirus-specified polypeptides Efficiency of expression of cDNAs of the reovirus S1 and S4 genes in transfected animal cells differs at the level of translation</title><author>Atwater, Jonathan A. ; Munemitsu, Susan M. ; Samuel, Charles E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-4d6795de00a729f9c70abe4e1a7aa6d1ea15d89e485fc70fa86560fc67ade47d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Cercopithecus aethiops</topic><topic>DNA - genetics</topic><topic>DNA, Recombinant</topic><topic>DNA, Viral - genetics</topic><topic>Genes, Viral</topic><topic>Kidney</topic><topic>Peptide Biosynthesis</topic><topic>Plasmids</topic><topic>Protein Biosynthesis</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Reoviridae - metabolism</topic><topic>reovirus</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Viral - genetics</topic><topic>Transfection</topic><topic>Viral Proteins - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atwater, Jonathan A.</creatorcontrib><creatorcontrib>Munemitsu, Susan M.</creatorcontrib><creatorcontrib>Samuel, Charles E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atwater, Jonathan A.</au><au>Munemitsu, Susan M.</au><au>Samuel, Charles E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosynthesis of reovirus-specified polypeptides Efficiency of expression of cDNAs of the reovirus S1 and S4 genes in transfected animal cells differs at the level of translation</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1987-08</date><risdate>1987</risdate><volume>159</volume><issue>2</issue><spage>350</spage><epage>357</epage><pages>350-357</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Full-length cDNAs of the reovirus serotype 1 Lang strain S1 and S4 genes were cloned in
Escherichia coli using bacteriophage M13 and expressed in monkey COS cells under the control of the SV40 late promoter using the eukaryotic expression vector pJC119. The
s1-encoded σ1 and
s4-encoded σ3 gene products were expressed in transfected COS cells and were indistinguishable from the authentic σ1 and σ3 polypeptides synthesized in reovirion-infected COS cells. The relative translational efficiencies of the
s1 and
s4 mRNAs in transfected COS cells were similar to the efficiencies observed in virion-infected cells; the
s4 mRNA was translated approximately five times more efficiently than the
s1 mRNA. Our results suggest that the differential translation of the reovirus
s1 and
s4 mRNAs
in vivo may be attributed to intrinsic structural properties of the individual mRNAs and is independent of competition with other viral mRNAs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>3617502</pmid><doi>10.1016/0042-6822(87)90473-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1987-08, Vol.159 (2), p.350-357 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77666528 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Cell Line Cercopithecus aethiops DNA - genetics DNA, Recombinant DNA, Viral - genetics Genes, Viral Kidney Peptide Biosynthesis Plasmids Protein Biosynthesis Recombinant Fusion Proteins - biosynthesis Reoviridae - metabolism reovirus RNA, Messenger - genetics RNA, Viral - genetics Transfection Viral Proteins - biosynthesis |
| title | Biosynthesis of reovirus-specified polypeptides Efficiency of expression of cDNAs of the reovirus S1 and S4 genes in transfected animal cells differs at the level of translation |
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