Sequences that confer β-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a β-tubulin mRNA
Synthesis of α- and β-tubulin is controlled in animal cells by a novel autoregulatory mechanism: the concentration of unpolymerized subunits specifies the level of tubulin mRNAs. Using transient DNA transfection, we have localized the sequences that identify a β-tubulin RNA as a substrate for autore...
Gespeichert in:
| Veröffentlicht in: | Cell 1987-08, Vol.50 (5), p.671-679 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 679 |
|---|---|
| container_issue | 5 |
| container_start_page | 671 |
| container_title | Cell |
| container_volume | 50 |
| creator | Gay, David A. Yen, Tim J. Lau, Joseph T.Y. Cleveland, Don W. |
| description | Synthesis of α- and β-tubulin is controlled in animal cells by a novel autoregulatory mechanism: the concentration of unpolymerized subunits specifies the level of tubulin mRNAs. Using transient DNA transfection, we have localized the sequences that identify a β-tubulin RNA as a substrate for autoregulation. Insertion of as few as 106 nucleotides (57 bases of 5′ untranslated region and 49 coding nucleotides) from a β-tubulin gene into a thymidine kinase gene is sufficient to make expression of the resultant chimeric RNA regulated as if it were an authentic β-tubulin mRNA. Furthermore, all 5′ untranslated region sequences can be deleted without disrupting regulation. We conclude that this novel autoregulatory pathway is specified by cytoplasmic events that modulate mRNA stability through sequences lying within the first 16 translated codons of a β-tubulin mRNA. |
| doi_str_mv | 10.1016/0092-8674(87)90325-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77660542</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0092867487903254</els_id><sourcerecordid>77660542</sourcerecordid><originalsourceid>FETCH-LOGICAL-c332t-10226217e1047bc49de7b9c0d0d76230134a03578315204cc2c36ce1fdfaabb63</originalsourceid><addsrcrecordid>eNqFkc1u1TAQhS1EVS6FNwDJC4RgERj_JE42SFXFn1SBxM_acuxJr1ESF9tpaR-LB-GZcLhXV6xgZcnzndGccwh5xOAFA9a8BOh41TZKPmvV8w4Eryt5h2wYdKqSTPG7ZHNA7pH7KX0DgLau62NyLBrOhBQbcvsZvy84W0w0b02mNswDRvrrZ5WXfhn9TM2SQ8SLZTTZh7lQMSwXWzoFt36ho9OnD6c0ZdP70ecbGjF5h_Ta521R44-iYTQM1Py9dNU8IEeDGRM-3L8n5Oub11_O3lXnH9--Pzs9r6wQPFcMOC_XKmQgVW9l51D1nQUHTjVcQPFhQNSqFazmIK3lVjQW2eAGY_q-ESfk6W7vZQzFa8p68sniOJoZw5K0Uk0DteT_BZnsQDGlCih3oI0hpYiDvox-MvFGM9BrN3oNXq_B61bpP91oWWSP9_uXfkJ3EO3LKPMn-7lJ1oxDNLP16YAp2RazbcFe7TAsoV15jDpZv1bofESbtQv-33f8BrekrCo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14907177</pqid></control><display><type>article</type><title>Sequences that confer β-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a β-tubulin mRNA</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Gay, David A. ; Yen, Tim J. ; Lau, Joseph T.Y. ; Cleveland, Don W.</creator><creatorcontrib>Gay, David A. ; Yen, Tim J. ; Lau, Joseph T.Y. ; Cleveland, Don W.</creatorcontrib><description>Synthesis of α- and β-tubulin is controlled in animal cells by a novel autoregulatory mechanism: the concentration of unpolymerized subunits specifies the level of tubulin mRNAs. Using transient DNA transfection, we have localized the sequences that identify a β-tubulin RNA as a substrate for autoregulation. Insertion of as few as 106 nucleotides (57 bases of 5′ untranslated region and 49 coding nucleotides) from a β-tubulin gene into a thymidine kinase gene is sufficient to make expression of the resultant chimeric RNA regulated as if it were an authentic β-tubulin mRNA. Furthermore, all 5′ untranslated region sequences can be deleted without disrupting regulation. We conclude that this novel autoregulatory pathway is specified by cytoplasmic events that modulate mRNA stability through sequences lying within the first 16 translated codons of a β-tubulin mRNA.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(87)90325-4</identifier><identifier>PMID: 3621343</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Codon ; DNA - genetics ; DNA, Recombinant ; Exons ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation ; Homeostasis ; L Cells (Cell Line) ; Mice ; Molecular and cellular biology ; Molecular genetics ; RNA, Messenger - genetics ; Transcription, Genetic ; Transfection ; Tubulin - genetics</subject><ispartof>Cell, 1987-08, Vol.50 (5), p.671-679</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-10226217e1047bc49de7b9c0d0d76230134a03578315204cc2c36ce1fdfaabb63</citedby><cites>FETCH-LOGICAL-c332t-10226217e1047bc49de7b9c0d0d76230134a03578315204cc2c36ce1fdfaabb63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0092867487903254$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7480138$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3621343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gay, David A.</creatorcontrib><creatorcontrib>Yen, Tim J.</creatorcontrib><creatorcontrib>Lau, Joseph T.Y.</creatorcontrib><creatorcontrib>Cleveland, Don W.</creatorcontrib><title>Sequences that confer β-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a β-tubulin mRNA</title><title>Cell</title><addtitle>Cell</addtitle><description>Synthesis of α- and β-tubulin is controlled in animal cells by a novel autoregulatory mechanism: the concentration of unpolymerized subunits specifies the level of tubulin mRNAs. Using transient DNA transfection, we have localized the sequences that identify a β-tubulin RNA as a substrate for autoregulation. Insertion of as few as 106 nucleotides (57 bases of 5′ untranslated region and 49 coding nucleotides) from a β-tubulin gene into a thymidine kinase gene is sufficient to make expression of the resultant chimeric RNA regulated as if it were an authentic β-tubulin mRNA. Furthermore, all 5′ untranslated region sequences can be deleted without disrupting regulation. We conclude that this novel autoregulatory pathway is specified by cytoplasmic events that modulate mRNA stability through sequences lying within the first 16 translated codons of a β-tubulin mRNA.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Codon</subject><subject>DNA - genetics</subject><subject>DNA, Recombinant</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Homeostasis</subject><subject>L Cells (Cell Line)</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>Tubulin - genetics</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQhS1EVS6FNwDJC4RgERj_JE42SFXFn1SBxM_acuxJr1ESF9tpaR-LB-GZcLhXV6xgZcnzndGccwh5xOAFA9a8BOh41TZKPmvV8w4Eryt5h2wYdKqSTPG7ZHNA7pH7KX0DgLau62NyLBrOhBQbcvsZvy84W0w0b02mNswDRvrrZ5WXfhn9TM2SQ8SLZTTZh7lQMSwXWzoFt36ho9OnD6c0ZdP70ecbGjF5h_Ta521R44-iYTQM1Py9dNU8IEeDGRM-3L8n5Oub11_O3lXnH9--Pzs9r6wQPFcMOC_XKmQgVW9l51D1nQUHTjVcQPFhQNSqFazmIK3lVjQW2eAGY_q-ESfk6W7vZQzFa8p68sniOJoZw5K0Uk0DteT_BZnsQDGlCih3oI0hpYiDvox-MvFGM9BrN3oNXq_B61bpP91oWWSP9_uXfkJ3EO3LKPMn-7lJ1oxDNLP16YAp2RazbcFe7TAsoV15jDpZv1bofESbtQv-33f8BrekrCo</recordid><startdate>19870828</startdate><enddate>19870828</enddate><creator>Gay, David A.</creator><creator>Yen, Tim J.</creator><creator>Lau, Joseph T.Y.</creator><creator>Cleveland, Don W.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19870828</creationdate><title>Sequences that confer β-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a β-tubulin mRNA</title><author>Gay, David A. ; Yen, Tim J. ; Lau, Joseph T.Y. ; Cleveland, Don W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-10226217e1047bc49de7b9c0d0d76230134a03578315204cc2c36ce1fdfaabb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Codon</topic><topic>DNA - genetics</topic><topic>DNA, Recombinant</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Homeostasis</topic><topic>L Cells (Cell Line)</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>Tubulin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gay, David A.</creatorcontrib><creatorcontrib>Yen, Tim J.</creatorcontrib><creatorcontrib>Lau, Joseph T.Y.</creatorcontrib><creatorcontrib>Cleveland, Don W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gay, David A.</au><au>Yen, Tim J.</au><au>Lau, Joseph T.Y.</au><au>Cleveland, Don W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequences that confer β-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a β-tubulin mRNA</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1987-08-28</date><risdate>1987</risdate><volume>50</volume><issue>5</issue><spage>671</spage><epage>679</epage><pages>671-679</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>Synthesis of α- and β-tubulin is controlled in animal cells by a novel autoregulatory mechanism: the concentration of unpolymerized subunits specifies the level of tubulin mRNAs. Using transient DNA transfection, we have localized the sequences that identify a β-tubulin RNA as a substrate for autoregulation. Insertion of as few as 106 nucleotides (57 bases of 5′ untranslated region and 49 coding nucleotides) from a β-tubulin gene into a thymidine kinase gene is sufficient to make expression of the resultant chimeric RNA regulated as if it were an authentic β-tubulin mRNA. Furthermore, all 5′ untranslated region sequences can be deleted without disrupting regulation. We conclude that this novel autoregulatory pathway is specified by cytoplasmic events that modulate mRNA stability through sequences lying within the first 16 translated codons of a β-tubulin mRNA.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>3621343</pmid><doi>10.1016/0092-8674(87)90325-4</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 1987-08, Vol.50 (5), p.671-679 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77660542 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Base Sequence Biological and medical sciences Codon DNA - genetics DNA, Recombinant Exons Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation Homeostasis L Cells (Cell Line) Mice Molecular and cellular biology Molecular genetics RNA, Messenger - genetics Transcription, Genetic Transfection Tubulin - genetics |
| title | Sequences that confer β-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a β-tubulin mRNA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T20%3A28%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sequences%20that%20confer%20%CE%B2-tubulin%20autoregulation%20through%20modulated%20mRNA%20stability%20reside%20within%20exon%201%20of%20a%20%CE%B2-tubulin%20mRNA&rft.jtitle=Cell&rft.au=Gay,%20David%20A.&rft.date=1987-08-28&rft.volume=50&rft.issue=5&rft.spage=671&rft.epage=679&rft.pages=671-679&rft.issn=0092-8674&rft.eissn=1097-4172&rft.coden=CELLB5&rft_id=info:doi/10.1016/0092-8674(87)90325-4&rft_dat=%3Cproquest_cross%3E77660542%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14907177&rft_id=info:pmid/3621343&rft_els_id=0092867487903254&rfr_iscdi=true |