The electrophoretic mobility of tripeptides as a function of pH and ionic strength : comparison with iontophoretic flux data
Capillary electrophoresis (CE) is an extremely efficient separations tool which can also be used to determine fundamental molecular parameters, e.g., the electrophoretic mobility of a molecule. We have studied the changes in the CE estimated electrophoretic mobility of thyrotropin releasing hormone...
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| Veröffentlicht in: | Pharmaceutical research 1995-05, Vol.12 (5), p.751-755 |
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| container_title | Pharmaceutical research |
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| creator | VANORMAN HUFF, B LIVERSIDGE, G. G MCINTIRE, G. L |
| description | Capillary electrophoresis (CE) is an extremely efficient separations tool which can also be used to determine fundamental molecular parameters, e.g., the electrophoretic mobility of a molecule. We have studied the changes in the CE estimated electrophoretic mobility of thyrotropin releasing hormone (TRH) as a function of pH and ionic strength. Further, we have used CE to estimate the mobilities of two synthetic analogs of TRH to examine the behavior of positive (basic) and negative (acidic) peptides under the conditions of this work. These data were then compared with literature values of iontophoretic flux of these molecules under similar formulation conditions. Our results suggest that CE could potentially assist formulation optimization for the iontophoretic delivery of peptides. |
| doi_str_mv | 10.1023/A:1016224028821 |
| format | Article |
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G ; MCINTIRE, G. L</creator><creatorcontrib>VANORMAN HUFF, B ; LIVERSIDGE, G. G ; MCINTIRE, G. L</creatorcontrib><description>Capillary electrophoresis (CE) is an extremely efficient separations tool which can also be used to determine fundamental molecular parameters, e.g., the electrophoretic mobility of a molecule. We have studied the changes in the CE estimated electrophoretic mobility of thyrotropin releasing hormone (TRH) as a function of pH and ionic strength. Further, we have used CE to estimate the mobilities of two synthetic analogs of TRH to examine the behavior of positive (basic) and negative (acidic) peptides under the conditions of this work. These data were then compared with literature values of iontophoretic flux of these molecules under similar formulation conditions. Our results suggest that CE could potentially assist formulation optimization for the iontophoretic delivery of peptides.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1023/A:1016224028821</identifier><identifier>PMID: 7479563</identifier><identifier>CODEN: PHREEB</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Amino Acid Sequence ; Biological and medical sciences ; Chemical Phenomena ; Chemistry, Physical ; Electrophoresis ; General pharmacology ; Humans ; Hydrogen-Ion Concentration ; Iontophoresis ; Kinetics ; Medical sciences ; Molecular Sequence Data ; Osmolar Concentration ; Peptides - chemistry ; Peptides - pharmacokinetics ; Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions ; Pharmacology. Drug treatments ; Skin - metabolism ; Skin Absorption ; Thyrotropin-Releasing Hormone - analogs & derivatives ; Thyrotropin-Releasing Hormone - chemistry ; Thyrotropin-Releasing Hormone - pharmacokinetics</subject><ispartof>Pharmaceutical research, 1995-05, Vol.12 (5), p.751-755</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3514015$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7479563$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VANORMAN HUFF, B</creatorcontrib><creatorcontrib>LIVERSIDGE, G. G</creatorcontrib><creatorcontrib>MCINTIRE, G. L</creatorcontrib><title>The electrophoretic mobility of tripeptides as a function of pH and ionic strength : comparison with iontophoretic flux data</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><description>Capillary electrophoresis (CE) is an extremely efficient separations tool which can also be used to determine fundamental molecular parameters, e.g., the electrophoretic mobility of a molecule. We have studied the changes in the CE estimated electrophoretic mobility of thyrotropin releasing hormone (TRH) as a function of pH and ionic strength. Further, we have used CE to estimate the mobilities of two synthetic analogs of TRH to examine the behavior of positive (basic) and negative (acidic) peptides under the conditions of this work. These data were then compared with literature values of iontophoretic flux of these molecules under similar formulation conditions. Our results suggest that CE could potentially assist formulation optimization for the iontophoretic delivery of peptides.</description><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Electrophoresis</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Iontophoresis</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Osmolar Concentration</subject><subject>Peptides - chemistry</subject><subject>Peptides - pharmacokinetics</subject><subject>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</subject><subject>Pharmacology. Drug treatments</subject><subject>Skin - metabolism</subject><subject>Skin Absorption</subject><subject>Thyrotropin-Releasing Hormone - analogs & derivatives</subject><subject>Thyrotropin-Releasing Hormone - chemistry</subject><subject>Thyrotropin-Releasing Hormone - pharmacokinetics</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtLAzEQh4MotVbPnoQcxNtqnptNb1LUCgUvFbwt2TxsZF8mWbTgH--Ki8LAMPN98zsMAOcYXWNE6M3tEiOcE8IQKQqCD8Acc0EzidjLIZgjQVhWCIaPwUmMbwihAks2AzPBhOQ5nYOv7c5CW1udQtfvumCT17DpKl_7tIedgyn43vbJGxuhGgu6odXJd-0P7NdQtQaO03gVU7Dta9rBJdRd06vg42h9-HEzCuk_3tXDJzQqqVNw5FQd7dnUF-D5_m67Wmebp4fH1e0m63EuUqYMpRXninIunNNai1wjJp2puM5NUVnEsMSq4sIopAR2TBKZM4EJosZJSRfg6je3D937YGMqGx-1rWvV2m6IpRA5J5KRUbyYxKFqrCn74BsV9uX0rpFfTlxFrWoXVKt9_NMoxwxhTr8BDeF7Sw</recordid><startdate>19950501</startdate><enddate>19950501</enddate><creator>VANORMAN HUFF, B</creator><creator>LIVERSIDGE, G. G</creator><creator>MCINTIRE, G. L</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19950501</creationdate><title>The electrophoretic mobility of tripeptides as a function of pH and ionic strength : comparison with iontophoretic flux data</title><author>VANORMAN HUFF, B ; LIVERSIDGE, G. G ; MCINTIRE, G. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p167t-ad33b55a3557ffccc76c049fdb5c6d8be04191ab57da0a71f49296471203df993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Electrophoresis</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Iontophoresis</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Osmolar Concentration</topic><topic>Peptides - chemistry</topic><topic>Peptides - pharmacokinetics</topic><topic>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</topic><topic>Pharmacology. Drug treatments</topic><topic>Skin - metabolism</topic><topic>Skin Absorption</topic><topic>Thyrotropin-Releasing Hormone - analogs & derivatives</topic><topic>Thyrotropin-Releasing Hormone - chemistry</topic><topic>Thyrotropin-Releasing Hormone - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VANORMAN HUFF, B</creatorcontrib><creatorcontrib>LIVERSIDGE, G. G</creatorcontrib><creatorcontrib>MCINTIRE, G. L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VANORMAN HUFF, B</au><au>LIVERSIDGE, G. G</au><au>MCINTIRE, G. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The electrophoretic mobility of tripeptides as a function of pH and ionic strength : comparison with iontophoretic flux data</atitle><jtitle>Pharmaceutical research</jtitle><addtitle>Pharm Res</addtitle><date>1995-05-01</date><risdate>1995</risdate><volume>12</volume><issue>5</issue><spage>751</spage><epage>755</epage><pages>751-755</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><coden>PHREEB</coden><abstract>Capillary electrophoresis (CE) is an extremely efficient separations tool which can also be used to determine fundamental molecular parameters, e.g., the electrophoretic mobility of a molecule. We have studied the changes in the CE estimated electrophoretic mobility of thyrotropin releasing hormone (TRH) as a function of pH and ionic strength. Further, we have used CE to estimate the mobilities of two synthetic analogs of TRH to examine the behavior of positive (basic) and negative (acidic) peptides under the conditions of this work. These data were then compared with literature values of iontophoretic flux of these molecules under similar formulation conditions. Our results suggest that CE could potentially assist formulation optimization for the iontophoretic delivery of peptides.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>7479563</pmid><doi>10.1023/A:1016224028821</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0724-8741 |
| ispartof | Pharmaceutical research, 1995-05, Vol.12 (5), p.751-755 |
| issn | 0724-8741 1573-904X |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amino Acid Sequence Biological and medical sciences Chemical Phenomena Chemistry, Physical Electrophoresis General pharmacology Humans Hydrogen-Ion Concentration Iontophoresis Kinetics Medical sciences Molecular Sequence Data Osmolar Concentration Peptides - chemistry Peptides - pharmacokinetics Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions Pharmacology. Drug treatments Skin - metabolism Skin Absorption Thyrotropin-Releasing Hormone - analogs & derivatives Thyrotropin-Releasing Hormone - chemistry Thyrotropin-Releasing Hormone - pharmacokinetics |
| title | The electrophoretic mobility of tripeptides as a function of pH and ionic strength : comparison with iontophoretic flux data |
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