A rapid assay method for ammonia using glutamine synthetase from glutamate-producing bacteria

A rapid enzymatic assay method for ammonia was developed by using glutamine synthetase from glutamate-producing bacteria together with pyruvate kinase, lactate dehydrogenase, and NADH. The time required for determination of 25 nmol of ammonia was 5 min with 1 unit of glutamine synthetase, as opposed...

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Veröffentlicht in:	Analytical biochemistry 1987-05, Vol.163 (1), p.117-122
Hauptverfasser:	Wakisaka, Shinji, Tachiki, Takashi, Sung, Ha-Chin, Kumagai, Hidehiko, Tochikura, Tatsurokuro, Matsui, Susumu
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - analysis Adenosine Triphosphate - analysis ADP ammonia Ammonia - analysis Analytical, structural and metabolic biochemistry Animals ATP Bacteria - enzymology Biological and medical sciences Brevibacterium - enzymology Cattle Fundamental and applied biological sciences. Psychology Glutamate Dehydrogenase - metabolism Glutamate-Ammonia Ligase - metabolism glutamine synthetase Intermediary metabolites. Miscellaneous Micrococcus - enzymology NAD - metabolism NADP - metabolism Other biological molecules pyruvate kinase Saccharomyces cerevisiae - enzymology spectophotometry Spectrophotometry Spectrophotometry, Ultraviolet Time Factors
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