Monoclonal Antibody Developed against a Hemolysin of Bacillus thuringiensis
A total of five hybridoma cell lines that produced monoclonal antibodies (MAb) against a hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis were established and characterized. All of these monoclonal antibodies reacted similarly not only to Bt-hemolysin but also to a hemolysin (Bc-hemolysin...
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| Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 1995, Vol.39(8), pp.619-622 |
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| container_title | MICROBIOLOGY and IMMUNOLOGY |
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| creator | Matsuyama, Junko Yamamoto, Koichiro Miwatani, Toshio Honda, Takeshi |
| description | A total of five hybridoma cell lines that produced monoclonal antibodies (MAb) against a hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis were established and characterized. All of these monoclonal antibodies reacted similarly not only to Bt-hemolysin but also to a hemolysin (Bc-hemolysin) produced by B. cereus, suggesting that the two hemolysins are immunologically indistinguishable. The MAb developed in this study was also successfully applied for rapid and simple purification of both Bt- and Bc-hemolysins by inununoaffinity column chromatography. The partial N-terminal amino acid sequence of the purified hemolysins was determined to be Ile-Glu-Gln-Thr. |
| doi_str_mv | 10.1111/j.1348-0421.1995.tb02251.x |
| format | Article |
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All of these monoclonal antibodies reacted similarly not only to Bt-hemolysin but also to a hemolysin (Bc-hemolysin) produced by B. cereus, suggesting that the two hemolysins are immunologically indistinguishable. The MAb developed in this study was also successfully applied for rapid and simple purification of both Bt- and Bc-hemolysins by inununoaffinity column chromatography. The partial N-terminal amino acid sequence of the purified hemolysins was determined to be Ile-Glu-Gln-Thr.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1995.tb02251.x</identifier><identifier>PMID: 7494502</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Antibodies, immunoglobulins ; Antibodies, Monoclonal - immunology ; Bacillus cereus ; Bacillus thuringiensis ; Bacillus thuringiensis - metabolism ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hemolysin ; Hemolysin Proteins - immunology ; Hybridomas ; Molecular immunology ; Monoclonal antibodies ; Monoclonal antibody</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1995, Vol.39(8), pp.619-622</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6581-f3b630f92e37733e260a3969315a93d25c40a3c3448a9d67aa00363e67a818bc3</citedby><cites>FETCH-LOGICAL-c6581-f3b630f92e37733e260a3969315a93d25c40a3c3448a9d67aa00363e67a818bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1882,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3653000$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7494502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuyama, Junko</creatorcontrib><creatorcontrib>Yamamoto, Koichiro</creatorcontrib><creatorcontrib>Miwatani, Toshio</creatorcontrib><creatorcontrib>Honda, Takeshi</creatorcontrib><title>Monoclonal Antibody Developed against a Hemolysin of Bacillus thuringiensis</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>A total of five hybridoma cell lines that produced monoclonal antibodies (MAb) against a hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis were established and characterized. All of these monoclonal antibodies reacted similarly not only to Bt-hemolysin but also to a hemolysin (Bc-hemolysin) produced by B. cereus, suggesting that the two hemolysins are immunologically indistinguishable. The MAb developed in this study was also successfully applied for rapid and simple purification of both Bt- and Bc-hemolysins by inununoaffinity column chromatography. The partial N-terminal amino acid sequence of the purified hemolysins was determined to be Ile-Glu-Gln-Thr.</description><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Bacillus cereus</subject><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis - metabolism</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hemolysin</subject><subject>Hemolysin Proteins - immunology</subject><subject>Hybridomas</subject><subject>Molecular immunology</subject><subject>Monoclonal antibodies</subject><subject>Monoclonal antibody</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkF-P1CAUxYnRrOPoRzBpjDG-tPKnQPHJdV1nN-5oTFZ9JAyls4wUxtLqzLeXsU3jk4k8wA3n3N-FA8AzBAuU1qtdgUhZ5bDEqEBC0KLfQIwpKg73wGKW7oMFJBXNKYPwIXgU4w5CzHFVnoEzXoqSQrwAH9bBB-2CVy47973dhPqYvTM_jQt7U2dqq6yPfaayK9MGd4zWZ6HJ3iptnRti1t8NnfVba3y08TF40CgXzZPpXIIv7y9vL67ym0-r64vzm1wzWqG8IRtGYCOwIZwTYjCDiggmCKJKkBpTXaYLTcqyUqJmXCkICSMmVRWqNposwYuRu-_Cj8HEXrY2auOc8iYMUXLOKKKYJ-PLfxpRKXB6ShqdrK9Hq-5CjJ1p5L6zreqOEkF5Cl3u5ClZeUpWnkKXU-jykJqfTnOGTWvquXVKOenPJ11FrVzTKa9tnG2EUQLTH5fgzWj7ZZ05_scD5Pp6_adMiMsRsYu92pqZobreamdkq3xtkeBcEiGrcWNIzLq-U500PnHykWNjbw5_Yb5Lxgmn8tvHlcSrr7eI8lJ-Jr8BpL3E0w</recordid><startdate>19950101</startdate><enddate>19950101</enddate><creator>Matsuyama, Junko</creator><creator>Yamamoto, Koichiro</creator><creator>Miwatani, Toshio</creator><creator>Honda, Takeshi</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19950101</creationdate><title>Monoclonal Antibody Developed against a Hemolysin of Bacillus thuringiensis</title><author>Matsuyama, Junko ; Yamamoto, Koichiro ; Miwatani, Toshio ; Honda, Takeshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6581-f3b630f92e37733e260a3969315a93d25c40a3c3448a9d67aa00363e67a818bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Antibodies, immunoglobulins</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Bacillus cereus</topic><topic>Bacillus thuringiensis</topic><topic>Bacillus thuringiensis - metabolism</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hemolysin</topic><topic>Hemolysin Proteins - immunology</topic><topic>Hybridomas</topic><topic>Molecular immunology</topic><topic>Monoclonal antibodies</topic><topic>Monoclonal antibody</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuyama, Junko</creatorcontrib><creatorcontrib>Yamamoto, Koichiro</creatorcontrib><creatorcontrib>Miwatani, Toshio</creatorcontrib><creatorcontrib>Honda, Takeshi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuyama, Junko</au><au>Yamamoto, Koichiro</au><au>Miwatani, Toshio</au><au>Honda, Takeshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal Antibody Developed against a Hemolysin of Bacillus thuringiensis</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1995-01-01</date><risdate>1995</risdate><volume>39</volume><issue>8</issue><spage>619</spage><epage>622</epage><pages>619-622</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>A total of five hybridoma cell lines that produced monoclonal antibodies (MAb) against a hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis were established and characterized. All of these monoclonal antibodies reacted similarly not only to Bt-hemolysin but also to a hemolysin (Bc-hemolysin) produced by B. cereus, suggesting that the two hemolysins are immunologically indistinguishable. The MAb developed in this study was also successfully applied for rapid and simple purification of both Bt- and Bc-hemolysins by inununoaffinity column chromatography. The partial N-terminal amino acid sequence of the purified hemolysins was determined to be Ile-Glu-Gln-Thr.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>7494502</pmid><doi>10.1111/j.1348-0421.1995.tb02251.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0385-5600 |
| ispartof | MICROBIOLOGY and IMMUNOLOGY, 1995, Vol.39(8), pp.619-622 |
| issn | 0385-5600 1348-0421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77651527 |
| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Alma/SFX Local Collection |
| subjects | Antibodies, immunoglobulins Antibodies, Monoclonal - immunology Bacillus cereus Bacillus thuringiensis Bacillus thuringiensis - metabolism Biological and medical sciences Fundamental and applied biological sciences. Psychology Fundamental immunology Hemolysin Hemolysin Proteins - immunology Hybridomas Molecular immunology Monoclonal antibodies Monoclonal antibody |
| title | Monoclonal Antibody Developed against a Hemolysin of Bacillus thuringiensis |
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