pALEX, a dual-tag prokaryotic expression vector for the purification of full-length proteins
pALEX, a prokaryotic expression vector, was constructed in which the multiple cloning site (MCS, polylinker) is flanked by sequences encoding glutathione S-transferase (GST) at the 5' end and a His 6 residue tag at the 3′ end. Open reading frames cloned into this vector can direct production of...
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| Veröffentlicht in: | Gene 1995-10, Vol.164 (1), p.45-47 |
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| container_title | Gene |
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| creator | Panagiotidis, Christos A. Silverstein, Saul J. |
| description | pALEX, a prokaryotic expression vector, was constructed in which the multiple cloning site (MCS, polylinker) is flanked by sequences encoding glutathione S-transferase (GST) at the 5' end and a His
6 residue tag at the 3′ end. Open reading frames cloned into this vector can direct production of fusion proteins with GST at their N terminus and a His
6 tag at their C terminus. This allows for the purification of full-size fusion proteins by a sequential two-step procedure on glutathione-agarose and Ni
2+-agarose columns. |
| doi_str_mv | 10.1016/0378-1119(95)00417-5 |
| format | Article |
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6 residue tag at the 3′ end. Open reading frames cloned into this vector can direct production of fusion proteins with GST at their N terminus and a His
6 tag at their C terminus. This allows for the purification of full-size fusion proteins by a sequential two-step procedure on glutathione-agarose and Ni
2+-agarose columns.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(95)00417-5</identifier><identifier>PMID: 7590319</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Base Sequence ; Cloning, Molecular - methods ; fusion protein ; Genetic Vectors ; Glutathione Transferase - genetics ; Immediate-Early Proteins - biosynthesis ; Immediate-Early Proteins - genetics ; Immediate-Early Proteins - isolation & purification ; Molecular Sequence Data ; Prokaryotic Cells ; protein purification ; Recombinant DNA ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - isolation & purification ; Ubiquitin-Protein Ligases</subject><ispartof>Gene, 1995-10, Vol.164 (1), p.45-47</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-eb8f711b9e95a6f5555c5779e1a4a024e360185d7476f7cde1605c63f17c90693</citedby><cites>FETCH-LOGICAL-c470t-eb8f711b9e95a6f5555c5779e1a4a024e360185d7476f7cde1605c63f17c90693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0378111995004175$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7590319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Panagiotidis, Christos A.</creatorcontrib><creatorcontrib>Silverstein, Saul J.</creatorcontrib><title>pALEX, a dual-tag prokaryotic expression vector for the purification of full-length proteins</title><title>Gene</title><addtitle>Gene</addtitle><description>pALEX, a prokaryotic expression vector, was constructed in which the multiple cloning site (MCS, polylinker) is flanked by sequences encoding glutathione S-transferase (GST) at the 5' end and a His
6 residue tag at the 3′ end. Open reading frames cloned into this vector can direct production of fusion proteins with GST at their N terminus and a His
6 tag at their C terminus. This allows for the purification of full-size fusion proteins by a sequential two-step procedure on glutathione-agarose and Ni
2+-agarose columns.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Cloning, Molecular - methods</subject><subject>fusion protein</subject><subject>Genetic Vectors</subject><subject>Glutathione Transferase - genetics</subject><subject>Immediate-Early Proteins - biosynthesis</subject><subject>Immediate-Early Proteins - genetics</subject><subject>Immediate-Early Proteins - isolation & purification</subject><subject>Molecular Sequence Data</subject><subject>Prokaryotic Cells</subject><subject>protein purification</subject><subject>Recombinant DNA</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>Ubiquitin-Protein Ligases</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9r3DAQxUVo2Wz-fIMEfCoNxMnM2rKsSyGEbVNYyCWBHAJCK48SpV7bleTQfPvK2SXHdmCYw3vzHvwYO0G4QMDqEgpR54gov0p-BlCiyPkem2MtZA5Q1J_Y_MOyzw5CeIE0nC9mbCa4hALlnD0OV6vlw3mms2bUbR71Uzb4_pf2b310JqM_g6cQXN9lr2Ri7zObNj5TNozeWWd0nLTeZnZs27yl7ik-TwmRXBeO2Ger20DHu3vI7r8v765v8tXtj5_XV6vclAJiTuvaCsS1JMl1ZXkaw4WQhLrUsCipqABr3ohSVFaYhrACbqrCojASKlkcsi_b3FT8e6QQ1cYFQ22rO-rHoISoSiFL-K8RBYBcIE_Gcms0vg_Bk1WDd5tERSGoib6a0KoJrZJcvdNX09vpLn9cb6j5eNrhTvq3rU6Jxqsjr4Jx1BlqnE94VdO7fxf8BQ2VkzY</recordid><startdate>19951016</startdate><enddate>19951016</enddate><creator>Panagiotidis, Christos A.</creator><creator>Silverstein, Saul J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19951016</creationdate><title>pALEX, a dual-tag prokaryotic expression vector for the purification of full-length proteins</title><author>Panagiotidis, Christos A. ; Silverstein, Saul J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-eb8f711b9e95a6f5555c5779e1a4a024e360185d7476f7cde1605c63f17c90693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Cloning, Molecular - methods</topic><topic>fusion protein</topic><topic>Genetic Vectors</topic><topic>Glutathione Transferase - genetics</topic><topic>Immediate-Early Proteins - biosynthesis</topic><topic>Immediate-Early Proteins - genetics</topic><topic>Immediate-Early Proteins - isolation & purification</topic><topic>Molecular Sequence Data</topic><topic>Prokaryotic Cells</topic><topic>protein purification</topic><topic>Recombinant DNA</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - isolation & purification</topic><topic>Ubiquitin-Protein Ligases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panagiotidis, Christos A.</creatorcontrib><creatorcontrib>Silverstein, Saul J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panagiotidis, Christos A.</au><au>Silverstein, Saul J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>pALEX, a dual-tag prokaryotic expression vector for the purification of full-length proteins</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1995-10-16</date><risdate>1995</risdate><volume>164</volume><issue>1</issue><spage>45</spage><epage>47</epage><pages>45-47</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>pALEX, a prokaryotic expression vector, was constructed in which the multiple cloning site (MCS, polylinker) is flanked by sequences encoding glutathione S-transferase (GST) at the 5' end and a His
6 residue tag at the 3′ end. Open reading frames cloned into this vector can direct production of fusion proteins with GST at their N terminus and a His
6 tag at their C terminus. This allows for the purification of full-size fusion proteins by a sequential two-step procedure on glutathione-agarose and Ni
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| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1995-10, Vol.164 (1), p.45-47 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77647940 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Base Sequence Cloning, Molecular - methods fusion protein Genetic Vectors Glutathione Transferase - genetics Immediate-Early Proteins - biosynthesis Immediate-Early Proteins - genetics Immediate-Early Proteins - isolation & purification Molecular Sequence Data Prokaryotic Cells protein purification Recombinant DNA Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - isolation & purification Ubiquitin-Protein Ligases |
| title | pALEX, a dual-tag prokaryotic expression vector for the purification of full-length proteins |
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