Developmental expression of G αo and G αs isoforms in PC12 cells: relationship to neurite outgrowth
Recent evidence suggests neuronal differentiation is associated with developmentally regulated changes in the expression of certain G-protein subtypes but the relationship of such changes to neuronal differentiation and/or neurite outgrowth is poorly understood. In this study, the appearance of the...
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| Veröffentlicht in: | Brain research. Developmental brain research 1995-08, Vol.88 (1), p.30-36 |
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| container_title | Brain research. Developmental brain research |
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| creator | Andreopoulos, S. Li, P.P. Warsh, J.J. |
| description | Recent evidence suggests neuronal differentiation is associated with developmentally regulated changes in the expression of certain G-protein subtypes but the relationship of such changes to neuronal differentiation and/or neurite outgrowth is poorly understood. In this study, the appearance of the G-protein
α
o and
α
s subunit isoforms was characterized in NGF-induced differentiating pheochromocytoma (PC)12 cells and correlated with neurite outgrowth, which was assessed with direct morphometric measures at 24-h intervals for a 5-day period and at 24, 48 and 72 h following NGF removal. Significant increases were observed in
α
o1 immunoreactive levels and the ratio of
α
o1
α
o2
immunoreactivities during NGF exposure but not in the levels of either the long or short isoform of
α
s. The former changes also correlated significantly with neurite length during NGF treatment. Furthermore, no significant changes were observed in the levels of α
o or
α
s isoforms following NGF removal. These findings suggest that
α
o isoforms are involved in neuritic extension in PC12 cells. |
| doi_str_mv | 10.1016/0165-3806(95)00068-O |
| format | Article |
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α
o and
α
s subunit isoforms was characterized in NGF-induced differentiating pheochromocytoma (PC)12 cells and correlated with neurite outgrowth, which was assessed with direct morphometric measures at 24-h intervals for a 5-day period and at 24, 48 and 72 h following NGF removal. Significant increases were observed in
α
o1 immunoreactive levels and the ratio of
α
o1
α
o2
immunoreactivities during NGF exposure but not in the levels of either the long or short isoform of
α
s. The former changes also correlated significantly with neurite length during NGF treatment. Furthermore, no significant changes were observed in the levels of α
o or
α
s isoforms following NGF removal. These findings suggest that
α
o isoforms are involved in neuritic extension in PC12 cells.</description><identifier>ISSN: 0165-3806</identifier><identifier>DOI: 10.1016/0165-3806(95)00068-O</identifier><identifier>PMID: 7493405</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Cell Differentiation ; Culture Media ; G-protein ; GTP-Binding Proteins - biosynthesis ; Immunoblotting ; Isomerism ; Molecular Sequence Data ; Nerve growth factor ; Nerve Growth Factors - pharmacology ; Neurites - metabolism ; Neurites - ultrastructure ; Neuronal development ; PC12 cell ; PC12 Cells ; Rats</subject><ispartof>Brain research. Developmental brain research, 1995-08, Vol.88 (1), p.30-36</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-78eb60f33a1754a0ea4f879bc775ca93d107853cd35289ae55e5f84c235df3143</citedby><cites>FETCH-LOGICAL-c272t-78eb60f33a1754a0ea4f879bc775ca93d107853cd35289ae55e5f84c235df3143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7493405$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andreopoulos, S.</creatorcontrib><creatorcontrib>Li, P.P.</creatorcontrib><creatorcontrib>Warsh, J.J.</creatorcontrib><title>Developmental expression of G αo and G αs isoforms in PC12 cells: relationship to neurite outgrowth</title><title>Brain research. Developmental brain research</title><addtitle>Brain Res Dev Brain Res</addtitle><description>Recent evidence suggests neuronal differentiation is associated with developmentally regulated changes in the expression of certain G-protein subtypes but the relationship of such changes to neuronal differentiation and/or neurite outgrowth is poorly understood. In this study, the appearance of the G-protein
α
o and
α
s subunit isoforms was characterized in NGF-induced differentiating pheochromocytoma (PC)12 cells and correlated with neurite outgrowth, which was assessed with direct morphometric measures at 24-h intervals for a 5-day period and at 24, 48 and 72 h following NGF removal. Significant increases were observed in
α
o1 immunoreactive levels and the ratio of
α
o1
α
o2
immunoreactivities during NGF exposure but not in the levels of either the long or short isoform of
α
s. The former changes also correlated significantly with neurite length during NGF treatment. Furthermore, no significant changes were observed in the levels of α
o or
α
s isoforms following NGF removal. These findings suggest that
α
o isoforms are involved in neuritic extension in PC12 cells.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Culture Media</subject><subject>G-protein</subject><subject>GTP-Binding Proteins - biosynthesis</subject><subject>Immunoblotting</subject><subject>Isomerism</subject><subject>Molecular Sequence Data</subject><subject>Nerve growth factor</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Neurites - metabolism</subject><subject>Neurites - ultrastructure</subject><subject>Neuronal development</subject><subject>PC12 cell</subject><subject>PC12 Cells</subject><subject>Rats</subject><issn>0165-3806</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1OwzAQhb0AlVK4AUheIVgE7DiOExZIqEBBqlQWsLZcZ0KNkjjYToFjcRHORPqjLllYHs2b98b-EDqh5JISml71h0csI-l5zi8IIWkWzfbQcNc-QIfev_cCZRkdoIFIcpYQPkRwB0uobFtDE1SF4at14L2xDbYlnuDfH4tVU6wrj423pXV1XzT4eUxjrKGq_DV2UKnQe_zCtDhY3EDnTABsu_Dm7GdYHKH9UlUejrf3CL0-3L-MH6PpbPI0vp1GOhZxiEQG85SUjCkqeKIIqKTMRD7XQnCtclZQIjLOdMF4nOUKOAdeZomOGS9KRhM2Qmeb3NbZjw58kLXxq0eqBmznpRBpImhM-sFkM6id9d5BKVtnauW-JSVyRVSu0MkVOplzuSYqZ73tdJvfzWsodqYtzl6_2ejQf3JpwEmvDTQaCuNAB1lY8_-CP41PiKc</recordid><startdate>19950828</startdate><enddate>19950828</enddate><creator>Andreopoulos, S.</creator><creator>Li, P.P.</creator><creator>Warsh, J.J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950828</creationdate><title>Developmental expression of G αo and G αs isoforms in PC12 cells: relationship to neurite outgrowth</title><author>Andreopoulos, S. ; Li, P.P. ; Warsh, J.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-78eb60f33a1754a0ea4f879bc775ca93d107853cd35289ae55e5f84c235df3143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Culture Media</topic><topic>G-protein</topic><topic>GTP-Binding Proteins - biosynthesis</topic><topic>Immunoblotting</topic><topic>Isomerism</topic><topic>Molecular Sequence Data</topic><topic>Nerve growth factor</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Neurites - metabolism</topic><topic>Neurites - ultrastructure</topic><topic>Neuronal development</topic><topic>PC12 cell</topic><topic>PC12 Cells</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andreopoulos, S.</creatorcontrib><creatorcontrib>Li, P.P.</creatorcontrib><creatorcontrib>Warsh, J.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Developmental brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andreopoulos, S.</au><au>Li, P.P.</au><au>Warsh, J.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental expression of G αo and G αs isoforms in PC12 cells: relationship to neurite outgrowth</atitle><jtitle>Brain research. Developmental brain research</jtitle><addtitle>Brain Res Dev Brain Res</addtitle><date>1995-08-28</date><risdate>1995</risdate><volume>88</volume><issue>1</issue><spage>30</spage><epage>36</epage><pages>30-36</pages><issn>0165-3806</issn><abstract>Recent evidence suggests neuronal differentiation is associated with developmentally regulated changes in the expression of certain G-protein subtypes but the relationship of such changes to neuronal differentiation and/or neurite outgrowth is poorly understood. In this study, the appearance of the G-protein
α
o and
α
s subunit isoforms was characterized in NGF-induced differentiating pheochromocytoma (PC)12 cells and correlated with neurite outgrowth, which was assessed with direct morphometric measures at 24-h intervals for a 5-day period and at 24, 48 and 72 h following NGF removal. Significant increases were observed in
α
o1 immunoreactive levels and the ratio of
α
o1
α
o2
immunoreactivities during NGF exposure but not in the levels of either the long or short isoform of
α
s. The former changes also correlated significantly with neurite length during NGF treatment. Furthermore, no significant changes were observed in the levels of α
o or
α
s isoforms following NGF removal. These findings suggest that
α
o isoforms are involved in neuritic extension in PC12 cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7493405</pmid><doi>10.1016/0165-3806(95)00068-O</doi><tpages>7</tpages></addata></record> |
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| ispartof | Brain research. Developmental brain research, 1995-08, Vol.88 (1), p.30-36 |
| issn | 0165-3806 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77647120 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Cell Differentiation Culture Media G-protein GTP-Binding Proteins - biosynthesis Immunoblotting Isomerism Molecular Sequence Data Nerve growth factor Nerve Growth Factors - pharmacology Neurites - metabolism Neurites - ultrastructure Neuronal development PC12 cell PC12 Cells Rats |
| title | Developmental expression of G αo and G αs isoforms in PC12 cells: relationship to neurite outgrowth |
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