Identification of β-adrenoceptor subtypes in bovine ovarian and myometrial cell membranes
Beta-adrenoceptor (β-AR) concentrations were measured in the ovary and in the myometrium of 36 adult Friesian cows using a radiometric assay. The β-AR content in both tissues was determined using the highly specific antagonist (−) [ 3H]CGP 12177 and the amounts of β-AR subtypes were discriminated in...
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| Veröffentlicht in: | British veterinary journal 1995-09, Vol.151 (5), p.567-578 |
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| creator | Re, G. Badino, P. Novelli, A. Canese, M.G. Girardi, C. |
| description | Beta-adrenoceptor (β-AR) concentrations were measured in the ovary and in the myometrium of 36 adult Friesian cows using a radiometric assay. The β-AR content in both tissues was determined using the highly specific antagonist (−) [
3H]CGP 12177 and the amounts of β-AR subtypes were discriminated in the presence of highly selective unlabelled ligands (CGP 20712A, ICI 118 551, CGP 25827A). Scatchard analysis revealed a good linearity and
K
d values suggested the existence of high affinity β-adrenergic sites in the bovine genital tract. Total β-AR concentrations in the ovary and in the myometrium were, respectively, 87 ± 7 (
sem) and 240 ± 27 fmol mg
−1 of membrane protein. β
2-AR concentrations were significantly higher (
P |
| doi_str_mv | 10.1016/S0007-1935(05)80028-4 |
| format | Article |
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3H]CGP 12177 and the amounts of β-AR subtypes were discriminated in the presence of highly selective unlabelled ligands (CGP 20712A, ICI 118 551, CGP 25827A). Scatchard analysis revealed a good linearity and
K
d values suggested the existence of high affinity β-adrenergic sites in the bovine genital tract. Total β-AR concentrations in the ovary and in the myometrium were, respectively, 87 ± 7 (
sem) and 240 ± 27 fmol mg
−1 of membrane protein. β
2-AR concentrations were significantly higher (
P<0.01) in the ovary (66 ± 5) and the myometrium (180 ± 29) than those of β
1-AR (21 ± 4 and 60 ± 5, respectively). Significant differences (
P<0.05) were also to be found between high affinity state β
2-AR and low affinity β
2-AR concentrations, but their values correlated negatively in the two different tissues. Natural and synthetic agonists inhibited (−) [
3H]CGP 12177 binding to β-AR in the following order of potency: (−) isoproterenol > (±) clenbuterol ⪖ (−) adrenaline ≫ ≫ (−) noradrenaline, whereas synthetic antagonists inhibited binding in the following order of potency: (−) propranolol ≫ (±)ICI 118 551 ≫≫ (±)CGP 20712A.</description><identifier>ISSN: 0007-1935</identifier><identifier>DOI: 10.1016/S0007-1935(05)80028-4</identifier><identifier>PMID: 8556316</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Beta-adrenoceptors ; Binding, Competitive ; bovine ; Cattle ; Cell Membrane - chemistry ; Female ; myometrium ; Myometrium - chemistry ; ovary ; Ovary - chemistry ; Radioligand Assay ; Receptors, Adrenergic, beta-1 - analysis ; Receptors, Adrenergic, beta-2 - analysis</subject><ispartof>British veterinary journal, 1995-09, Vol.151 (5), p.567-578</ispartof><rights>1995 Bailière Tindall</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-cf1373374de461be99e7c8e768d2d822235be81bbb08646f72038523b6d774f3</citedby><cites>FETCH-LOGICAL-c360t-cf1373374de461be99e7c8e768d2d822235be81bbb08646f72038523b6d774f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8556316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Re, G.</creatorcontrib><creatorcontrib>Badino, P.</creatorcontrib><creatorcontrib>Novelli, A.</creatorcontrib><creatorcontrib>Canese, M.G.</creatorcontrib><creatorcontrib>Girardi, C.</creatorcontrib><title>Identification of β-adrenoceptor subtypes in bovine ovarian and myometrial cell membranes</title><title>British veterinary journal</title><addtitle>Br Vet J</addtitle><description>Beta-adrenoceptor (β-AR) concentrations were measured in the ovary and in the myometrium of 36 adult Friesian cows using a radiometric assay. The β-AR content in both tissues was determined using the highly specific antagonist (−) [
3H]CGP 12177 and the amounts of β-AR subtypes were discriminated in the presence of highly selective unlabelled ligands (CGP 20712A, ICI 118 551, CGP 25827A). Scatchard analysis revealed a good linearity and
K
d values suggested the existence of high affinity β-adrenergic sites in the bovine genital tract. Total β-AR concentrations in the ovary and in the myometrium were, respectively, 87 ± 7 (
sem) and 240 ± 27 fmol mg
−1 of membrane protein. β
2-AR concentrations were significantly higher (
P<0.01) in the ovary (66 ± 5) and the myometrium (180 ± 29) than those of β
1-AR (21 ± 4 and 60 ± 5, respectively). Significant differences (
P<0.05) were also to be found between high affinity state β
2-AR and low affinity β
2-AR concentrations, but their values correlated negatively in the two different tissues. Natural and synthetic agonists inhibited (−) [
3H]CGP 12177 binding to β-AR in the following order of potency: (−) isoproterenol > (±) clenbuterol ⪖ (−) adrenaline ≫ ≫ (−) noradrenaline, whereas synthetic antagonists inhibited binding in the following order of potency: (−) propranolol ≫ (±)ICI 118 551 ≫≫ (±)CGP 20712A.</description><subject>Animals</subject><subject>Beta-adrenoceptors</subject><subject>Binding, Competitive</subject><subject>bovine</subject><subject>Cattle</subject><subject>Cell Membrane - chemistry</subject><subject>Female</subject><subject>myometrium</subject><subject>Myometrium - chemistry</subject><subject>ovary</subject><subject>Ovary - chemistry</subject><subject>Radioligand Assay</subject><subject>Receptors, Adrenergic, beta-1 - analysis</subject><subject>Receptors, Adrenergic, beta-2 - analysis</subject><issn>0007-1935</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlKBDEQhnNQ3B9ByEn00Jqls_RJZHAZEDzoyUvoJNUQmU7GpGdgXssH8ZnsWZirp6KWv_6qD6FLSm4pofLunRCiKtpwcU3EjSaE6ao-QCf78jE6LeWLkFrKhh2hIy2E5FSeoM-phziELrh2CCni1OHfn6r1GWJyMB9SxmVhh9UcCg4R27QMEXBatjm0EbfR436VehjGdIYdzGa4h97mNkI5R4ddOytwsYtn6OPp8WPyUr2-PU8nD6-V45IMlesoV5yr2kMtqYWmAeU0KKk985oxxoUFTa21RMtadooRrgXjVnql6o6foavt2nlO3wsog-lDWV8y3pAWxSglayYbMQ6K7aDLqZQMnZnn0Ld5ZSgxa4xmg9GseRkizAajqUfd5c5gYXvwe9WO4di_3_ZhfHIZIJviAkQHPmRwg_Ep_OPwB2zihV0</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>Re, G.</creator><creator>Badino, P.</creator><creator>Novelli, A.</creator><creator>Canese, M.G.</creator><creator>Girardi, C.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950901</creationdate><title>Identification of β-adrenoceptor subtypes in bovine ovarian and myometrial cell membranes</title><author>Re, G. ; Badino, P. ; Novelli, A. ; Canese, M.G. ; Girardi, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-cf1373374de461be99e7c8e768d2d822235be81bbb08646f72038523b6d774f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Beta-adrenoceptors</topic><topic>Binding, Competitive</topic><topic>bovine</topic><topic>Cattle</topic><topic>Cell Membrane - chemistry</topic><topic>Female</topic><topic>myometrium</topic><topic>Myometrium - chemistry</topic><topic>ovary</topic><topic>Ovary - chemistry</topic><topic>Radioligand Assay</topic><topic>Receptors, Adrenergic, beta-1 - analysis</topic><topic>Receptors, Adrenergic, beta-2 - analysis</topic><toplevel>online_resources</toplevel><creatorcontrib>Re, G.</creatorcontrib><creatorcontrib>Badino, P.</creatorcontrib><creatorcontrib>Novelli, A.</creatorcontrib><creatorcontrib>Canese, M.G.</creatorcontrib><creatorcontrib>Girardi, C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>British veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Re, G.</au><au>Badino, P.</au><au>Novelli, A.</au><au>Canese, M.G.</au><au>Girardi, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of β-adrenoceptor subtypes in bovine ovarian and myometrial cell membranes</atitle><jtitle>British veterinary journal</jtitle><addtitle>Br Vet J</addtitle><date>1995-09-01</date><risdate>1995</risdate><volume>151</volume><issue>5</issue><spage>567</spage><epage>578</epage><pages>567-578</pages><issn>0007-1935</issn><abstract>Beta-adrenoceptor (β-AR) concentrations were measured in the ovary and in the myometrium of 36 adult Friesian cows using a radiometric assay. The β-AR content in both tissues was determined using the highly specific antagonist (−) [
3H]CGP 12177 and the amounts of β-AR subtypes were discriminated in the presence of highly selective unlabelled ligands (CGP 20712A, ICI 118 551, CGP 25827A). Scatchard analysis revealed a good linearity and
K
d values suggested the existence of high affinity β-adrenergic sites in the bovine genital tract. Total β-AR concentrations in the ovary and in the myometrium were, respectively, 87 ± 7 (
sem) and 240 ± 27 fmol mg
−1 of membrane protein. β
2-AR concentrations were significantly higher (
P<0.01) in the ovary (66 ± 5) and the myometrium (180 ± 29) than those of β
1-AR (21 ± 4 and 60 ± 5, respectively). Significant differences (
P<0.05) were also to be found between high affinity state β
2-AR and low affinity β
2-AR concentrations, but their values correlated negatively in the two different tissues. Natural and synthetic agonists inhibited (−) [
3H]CGP 12177 binding to β-AR in the following order of potency: (−) isoproterenol > (±) clenbuterol ⪖ (−) adrenaline ≫ ≫ (−) noradrenaline, whereas synthetic antagonists inhibited binding in the following order of potency: (−) propranolol ≫ (±)ICI 118 551 ≫≫ (±)CGP 20712A.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>8556316</pmid><doi>10.1016/S0007-1935(05)80028-4</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0007-1935 |
| ispartof | British veterinary journal, 1995-09, Vol.151 (5), p.567-578 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Beta-adrenoceptors Binding, Competitive bovine Cattle Cell Membrane - chemistry Female myometrium Myometrium - chemistry ovary Ovary - chemistry Radioligand Assay Receptors, Adrenergic, beta-1 - analysis Receptors, Adrenergic, beta-2 - analysis |
| title | Identification of β-adrenoceptor subtypes in bovine ovarian and myometrial cell membranes |
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