Demonstration of specific E-type prostaglandin receptors using enriched preparations of canine parietal cells and [ 3H] misoprostol free acid
High-affinity, E-type prostaglandin binding sites in enriched canine parietal cell preparations were identified with [ 3H] misoprostol free acid, a prostaglandin E 1 analogue. Saturable, reversible, and highly stereospecific binding was identified, with approximately 8,000 binding sites per cell. Pr...
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| Veröffentlicht in: | The American journal of medicine 1987-07, Vol.83 (1), p.9-14 |
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| container_title | The American journal of medicine |
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| creator | Tsai, Bie S. Kessler, Linda K. Schoenhard, Grant Collins, Paul W. Bauer, Raymond F. |
| description | High-affinity, E-type prostaglandin binding sites in enriched canine parietal cell preparations were identified with [
3H] misoprostol free acid, a prostaglandin E
1 analogue. Saturable, reversible, and highly stereospecific binding was identified, with approximately 8,000 binding sites per cell. Prostaglandin I and F bound weakly, and cimetidine and histamine did not bind. The results indicate that [
3H] misoprostol free acid binds to E-type prostaglandin receptors, which suggests that the ulcer-healing inhibition of gastric acid secretion by misoprostol results from its interaction with a specific E-type prostaglandin receptor. |
| doi_str_mv | 10.1016/0002-9343(87)90572-9 |
| format | Article |
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3H] misoprostol free acid, a prostaglandin E
1 analogue. Saturable, reversible, and highly stereospecific binding was identified, with approximately 8,000 binding sites per cell. Prostaglandin I and F bound weakly, and cimetidine and histamine did not bind. The results indicate that [
3H] misoprostol free acid binds to E-type prostaglandin receptors, which suggests that the ulcer-healing inhibition of gastric acid secretion by misoprostol results from its interaction with a specific E-type prostaglandin receptor.</description><identifier>ISSN: 0002-9343</identifier><identifier>EISSN: 1555-7162</identifier><identifier>DOI: 10.1016/0002-9343(87)90572-9</identifier><identifier>PMID: 2887113</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alprostadil - analogs & derivatives ; Alprostadil - metabolism ; Alprostadil - pharmacology ; Animals ; Anti-Ulcer Agents - metabolism ; Dogs ; Misoprostol ; Parietal Cells, Gastric - analysis ; Prostaglandins E - pharmacology ; Receptors, Prostaglandin - analysis ; Receptors, Prostaglandin - drug effects ; Receptors, Prostaglandin E ; Tritium</subject><ispartof>The American journal of medicine, 1987-07, Vol.83 (1), p.9-14</ispartof><rights>1987</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-34291a75c9d3cf617be19875d6bcfe15c7e7d11a644d12d91f2bfb5a52ccf32f3</citedby><cites>FETCH-LOGICAL-c357t-34291a75c9d3cf617be19875d6bcfe15c7e7d11a644d12d91f2bfb5a52ccf32f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0002934387905729$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2887113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsai, Bie S.</creatorcontrib><creatorcontrib>Kessler, Linda K.</creatorcontrib><creatorcontrib>Schoenhard, Grant</creatorcontrib><creatorcontrib>Collins, Paul W.</creatorcontrib><creatorcontrib>Bauer, Raymond F.</creatorcontrib><title>Demonstration of specific E-type prostaglandin receptors using enriched preparations of canine parietal cells and [ 3H] misoprostol free acid</title><title>The American journal of medicine</title><addtitle>Am J Med</addtitle><description>High-affinity, E-type prostaglandin binding sites in enriched canine parietal cell preparations were identified with [
3H] misoprostol free acid, a prostaglandin E
1 analogue. Saturable, reversible, and highly stereospecific binding was identified, with approximately 8,000 binding sites per cell. Prostaglandin I and F bound weakly, and cimetidine and histamine did not bind. The results indicate that [
3H] misoprostol free acid binds to E-type prostaglandin receptors, which suggests that the ulcer-healing inhibition of gastric acid secretion by misoprostol results from its interaction with a specific E-type prostaglandin receptor.</description><subject>Alprostadil - analogs & derivatives</subject><subject>Alprostadil - metabolism</subject><subject>Alprostadil - pharmacology</subject><subject>Animals</subject><subject>Anti-Ulcer Agents - metabolism</subject><subject>Dogs</subject><subject>Misoprostol</subject><subject>Parietal Cells, Gastric - analysis</subject><subject>Prostaglandins E - pharmacology</subject><subject>Receptors, Prostaglandin - analysis</subject><subject>Receptors, Prostaglandin - drug effects</subject><subject>Receptors, Prostaglandin E</subject><subject>Tritium</subject><issn>0002-9343</issn><issn>1555-7162</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctqHDEQRUVIsMeT_EECWoV40bYerVb3xhAcv8CQTbIKQailkiPTI7UlTcAf4X-22jN46ZUo6tbRrVsIfabkhBLanRJCWDPwln_r5fFAhKzVO7SiQohG0o69R6tXySE6yvm-lmQQ3QE6YH0vKeUr9PQDNjHkknTxMeDocJ7BeOcNvmjK4wx4TjEXfTfpYH3ACQzMJaaMt9mHOwwhefMPbJXBrHeUvGCMDj7UaZ08FD1hA9OUcYXgP5hf_8Ubn-MLOk7YJQCsjbcf0Qenpwyf9u8a_b68-HV-3dz-vLo5_37bGC5kaXjLBqqlMIPlxnVUjkCHXgrbjcYBFUaCtJTqrm0tZXagjo1uFFowYxxnjq_R1x23OnjYQi6q2lkc6gBxm5WUXUsGJquw3QlNtZoTODUnv9HpUVGiliuoJWK1RKx6qV6uUKs1-rLnb8cN2Nehfey1f7brQ13yv4eksvEQDFhfAy7KRv_2B8_kXpm8</recordid><startdate>19870727</startdate><enddate>19870727</enddate><creator>Tsai, Bie S.</creator><creator>Kessler, Linda K.</creator><creator>Schoenhard, Grant</creator><creator>Collins, Paul W.</creator><creator>Bauer, Raymond F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870727</creationdate><title>Demonstration of specific E-type prostaglandin receptors using enriched preparations of canine parietal cells and [ 3H] misoprostol free acid</title><author>Tsai, Bie S. ; Kessler, Linda K. ; Schoenhard, Grant ; Collins, Paul W. ; Bauer, Raymond F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-34291a75c9d3cf617be19875d6bcfe15c7e7d11a644d12d91f2bfb5a52ccf32f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Alprostadil - analogs & derivatives</topic><topic>Alprostadil - metabolism</topic><topic>Alprostadil - pharmacology</topic><topic>Animals</topic><topic>Anti-Ulcer Agents - metabolism</topic><topic>Dogs</topic><topic>Misoprostol</topic><topic>Parietal Cells, Gastric - analysis</topic><topic>Prostaglandins E - pharmacology</topic><topic>Receptors, Prostaglandin - analysis</topic><topic>Receptors, Prostaglandin - drug effects</topic><topic>Receptors, Prostaglandin E</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsai, Bie S.</creatorcontrib><creatorcontrib>Kessler, Linda K.</creatorcontrib><creatorcontrib>Schoenhard, Grant</creatorcontrib><creatorcontrib>Collins, Paul W.</creatorcontrib><creatorcontrib>Bauer, Raymond F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsai, Bie S.</au><au>Kessler, Linda K.</au><au>Schoenhard, Grant</au><au>Collins, Paul W.</au><au>Bauer, Raymond F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Demonstration of specific E-type prostaglandin receptors using enriched preparations of canine parietal cells and [ 3H] misoprostol free acid</atitle><jtitle>The American journal of medicine</jtitle><addtitle>Am J Med</addtitle><date>1987-07-27</date><risdate>1987</risdate><volume>83</volume><issue>1</issue><spage>9</spage><epage>14</epage><pages>9-14</pages><issn>0002-9343</issn><eissn>1555-7162</eissn><abstract>High-affinity, E-type prostaglandin binding sites in enriched canine parietal cell preparations were identified with [
3H] misoprostol free acid, a prostaglandin E
1 analogue. Saturable, reversible, and highly stereospecific binding was identified, with approximately 8,000 binding sites per cell. Prostaglandin I and F bound weakly, and cimetidine and histamine did not bind. The results indicate that [
3H] misoprostol free acid binds to E-type prostaglandin receptors, which suggests that the ulcer-healing inhibition of gastric acid secretion by misoprostol results from its interaction with a specific E-type prostaglandin receptor.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>2887113</pmid><doi>10.1016/0002-9343(87)90572-9</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9343 |
| ispartof | The American journal of medicine, 1987-07, Vol.83 (1), p.9-14 |
| issn | 0002-9343 1555-7162 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alprostadil - analogs & derivatives Alprostadil - metabolism Alprostadil - pharmacology Animals Anti-Ulcer Agents - metabolism Dogs Misoprostol Parietal Cells, Gastric - analysis Prostaglandins E - pharmacology Receptors, Prostaglandin - analysis Receptors, Prostaglandin - drug effects Receptors, Prostaglandin E Tritium |
| title | Demonstration of specific E-type prostaglandin receptors using enriched preparations of canine parietal cells and [ 3H] misoprostol free acid |
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