Evidence for the presence of the alternatively spliced HLA-G mRNA forms in human mononuclear cells from peripheral blood and umbilical cord blood
The HLA-G monomorphic, nonclassic HLA class I gene encodes the molecule that is the only major histocompatibility complex antigen expressed on cytotrophoblasts of placenta. This restricted expression on fetal tissue that is in contact with maternal tissue suggests that HLA-G products may play a role...
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| Veröffentlicht in: | Human immunology 1995-07, Vol.43 (3), p.237-241 |
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| container_title | Human immunology |
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| creator | Kirszenbaum, Marek Moreau, Philippe Teyssier, Magali Lafon, Catherine Gluckman, Eliane Dausset, Jean Carosella, Edgardo |
| description | The HLA-G monomorphic, nonclassic HLA class I gene encodes the molecule that is the only major histocompatibility complex antigen expressed on cytotrophoblasts of placenta. This restricted expression on fetal tissue that is in contact with maternal tissue suggests that HLA-G products may play a role in maternofetal tolerance. We previously have demonstrated in firsttrimester human trophoblasts a new alternatively spliced form of HLA-G mRNA lacking exon 4 (HLA-G4) and weak expression of HLA-G1 copy mRNA in adult peripheral blood lymphocytes. By using exon-specific HLA-G primers, we demonstrate in this work the presence of three additional alternatively spliced forms of HLA-G mRNA in human peripheral mononuclear cells (HLA-G2, HLA-G3, and HLA-G4). In umbilical cord blood, HLA-G transcription was observed at very low level and only three alternatively spliced forms were detected (HLA-G1, HLA-G2, and HLA-G3). In contrast, we did not revealed any HLA-G transcript in CD34
+ fraction of cord blood cells. |
| doi_str_mv | 10.1016/0198-8859(95)00008-R |
| format | Article |
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+ fraction of cord blood cells.</description><identifier>ISSN: 0198-8859</identifier><identifier>EISSN: 1879-1166</identifier><identifier>DOI: 10.1016/0198-8859(95)00008-R</identifier><identifier>PMID: 7558942</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alternative Splicing ; Antigens, CD34 - biosynthesis ; Antigens, CD34 - genetics ; Base Sequence ; DNA Probes, HLA ; Exons ; Fetal Blood - immunology ; Fetal Blood - metabolism ; Gene Expression ; Histocompatibility Antigens Class I - biosynthesis ; Histocompatibility Antigens Class I - genetics ; HLA Antigens - biosynthesis ; HLA Antigens - genetics ; HLA-G Antigens ; Humans ; Infant, Newborn ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - metabolism ; Male ; Molecular Sequence Data ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics</subject><ispartof>Human immunology, 1995-07, Vol.43 (3), p.237-241</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-e37eeaf85504fb1bcfd50e39cc429656e69805ae190bfd7391c416113a2c10e3</citedby><cites>FETCH-LOGICAL-c303t-e37eeaf85504fb1bcfd50e39cc429656e69805ae190bfd7391c416113a2c10e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0198-8859(95)00008-R$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7558942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kirszenbaum, Marek</creatorcontrib><creatorcontrib>Moreau, Philippe</creatorcontrib><creatorcontrib>Teyssier, Magali</creatorcontrib><creatorcontrib>Lafon, Catherine</creatorcontrib><creatorcontrib>Gluckman, Eliane</creatorcontrib><creatorcontrib>Dausset, Jean</creatorcontrib><creatorcontrib>Carosella, Edgardo</creatorcontrib><title>Evidence for the presence of the alternatively spliced HLA-G mRNA forms in human mononuclear cells from peripheral blood and umbilical cord blood</title><title>Human immunology</title><addtitle>Hum Immunol</addtitle><description>The HLA-G monomorphic, nonclassic HLA class I gene encodes the molecule that is the only major histocompatibility complex antigen expressed on cytotrophoblasts of placenta. This restricted expression on fetal tissue that is in contact with maternal tissue suggests that HLA-G products may play a role in maternofetal tolerance. We previously have demonstrated in firsttrimester human trophoblasts a new alternatively spliced form of HLA-G mRNA lacking exon 4 (HLA-G4) and weak expression of HLA-G1 copy mRNA in adult peripheral blood lymphocytes. By using exon-specific HLA-G primers, we demonstrate in this work the presence of three additional alternatively spliced forms of HLA-G mRNA in human peripheral mononuclear cells (HLA-G2, HLA-G3, and HLA-G4). In umbilical cord blood, HLA-G transcription was observed at very low level and only three alternatively spliced forms were detected (HLA-G1, HLA-G2, and HLA-G3). In contrast, we did not revealed any HLA-G transcript in CD34
+ fraction of cord blood cells.</description><subject>Alternative Splicing</subject><subject>Antigens, CD34 - biosynthesis</subject><subject>Antigens, CD34 - genetics</subject><subject>Base Sequence</subject><subject>DNA Probes, HLA</subject><subject>Exons</subject><subject>Fetal Blood - immunology</subject><subject>Fetal Blood - metabolism</subject><subject>Gene Expression</subject><subject>Histocompatibility Antigens Class I - biosynthesis</subject><subject>Histocompatibility Antigens Class I - genetics</subject><subject>HLA Antigens - biosynthesis</subject><subject>HLA Antigens - genetics</subject><subject>HLA-G Antigens</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><issn>0198-8859</issn><issn>1879-1166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rHCEYx6U0pNu03yAFT6U9TKo7o6OXwhLSpLC0sOQujj6yBh2nOrOQj9FvnJndJcfWi_j8XxR_CF1TckMJ5d8IlaISgskvkn0l8xLV7g1aUdHKilLO36LVq-Udel_K0-xpSdtcosuWMSGb9Qr9vTt4C70B7FLG4x7wkKEcB8kdzzqMkHs9-gOEZ1yG4A1Y_LDdVPc47n5tlmAs2Pd4P0Xd45j61E8mgM7YQAgFu5wiHiD7YQ9ZB9yFlCzWvcVT7PzcN89MyvYkfEAXTocCH8_7FXr8cfd4-1Btf9__vN1sK1OTeqygbgG0E4yRxnW0M84yArU0pllLzjhwKQjTQCXpnG1rSU1DOaW1Xhs6G6_Q51PtkNOfCcqooi_Le3UPaSqqbXnNJK__a6RcMEJbMRubk9HkVEoGp4bso87PihK1EFMLDrXgUJKpIzG1m2Ofzv1TF8G-hs6IZv37SYf5Mw4esirGL4Csz2BGZZP_9wUvsbCnIw</recordid><startdate>199507</startdate><enddate>199507</enddate><creator>Kirszenbaum, Marek</creator><creator>Moreau, Philippe</creator><creator>Teyssier, Magali</creator><creator>Lafon, Catherine</creator><creator>Gluckman, Eliane</creator><creator>Dausset, Jean</creator><creator>Carosella, Edgardo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199507</creationdate><title>Evidence for the presence of the alternatively spliced HLA-G mRNA forms in human mononuclear cells from peripheral blood and umbilical cord blood</title><author>Kirszenbaum, Marek ; Moreau, Philippe ; Teyssier, Magali ; Lafon, Catherine ; Gluckman, Eliane ; Dausset, Jean ; Carosella, Edgardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-e37eeaf85504fb1bcfd50e39cc429656e69805ae190bfd7391c416113a2c10e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Alternative Splicing</topic><topic>Antigens, CD34 - biosynthesis</topic><topic>Antigens, CD34 - genetics</topic><topic>Base Sequence</topic><topic>DNA Probes, HLA</topic><topic>Exons</topic><topic>Fetal Blood - immunology</topic><topic>Fetal Blood - metabolism</topic><topic>Gene Expression</topic><topic>Histocompatibility Antigens Class I - biosynthesis</topic><topic>Histocompatibility Antigens Class I - genetics</topic><topic>HLA Antigens - biosynthesis</topic><topic>HLA Antigens - genetics</topic><topic>HLA-G Antigens</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kirszenbaum, Marek</creatorcontrib><creatorcontrib>Moreau, Philippe</creatorcontrib><creatorcontrib>Teyssier, Magali</creatorcontrib><creatorcontrib>Lafon, Catherine</creatorcontrib><creatorcontrib>Gluckman, Eliane</creatorcontrib><creatorcontrib>Dausset, Jean</creatorcontrib><creatorcontrib>Carosella, Edgardo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kirszenbaum, Marek</au><au>Moreau, Philippe</au><au>Teyssier, Magali</au><au>Lafon, Catherine</au><au>Gluckman, Eliane</au><au>Dausset, Jean</au><au>Carosella, Edgardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for the presence of the alternatively spliced HLA-G mRNA forms in human mononuclear cells from peripheral blood and umbilical cord blood</atitle><jtitle>Human immunology</jtitle><addtitle>Hum Immunol</addtitle><date>1995-07</date><risdate>1995</risdate><volume>43</volume><issue>3</issue><spage>237</spage><epage>241</epage><pages>237-241</pages><issn>0198-8859</issn><eissn>1879-1166</eissn><abstract>The HLA-G monomorphic, nonclassic HLA class I gene encodes the molecule that is the only major histocompatibility complex antigen expressed on cytotrophoblasts of placenta. This restricted expression on fetal tissue that is in contact with maternal tissue suggests that HLA-G products may play a role in maternofetal tolerance. We previously have demonstrated in firsttrimester human trophoblasts a new alternatively spliced form of HLA-G mRNA lacking exon 4 (HLA-G4) and weak expression of HLA-G1 copy mRNA in adult peripheral blood lymphocytes. By using exon-specific HLA-G primers, we demonstrate in this work the presence of three additional alternatively spliced forms of HLA-G mRNA in human peripheral mononuclear cells (HLA-G2, HLA-G3, and HLA-G4). In umbilical cord blood, HLA-G transcription was observed at very low level and only three alternatively spliced forms were detected (HLA-G1, HLA-G2, and HLA-G3). In contrast, we did not revealed any HLA-G transcript in CD34
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| fulltext | fulltext |
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| ispartof | Human immunology, 1995-07, Vol.43 (3), p.237-241 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Alternative Splicing Antigens, CD34 - biosynthesis Antigens, CD34 - genetics Base Sequence DNA Probes, HLA Exons Fetal Blood - immunology Fetal Blood - metabolism Gene Expression Histocompatibility Antigens Class I - biosynthesis Histocompatibility Antigens Class I - genetics HLA Antigens - biosynthesis HLA Antigens - genetics HLA-G Antigens Humans Infant, Newborn Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism Male Molecular Sequence Data RNA, Messenger - biosynthesis RNA, Messenger - genetics |
| title | Evidence for the presence of the alternatively spliced HLA-G mRNA forms in human mononuclear cells from peripheral blood and umbilical cord blood |
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