Comparison of human immunodeficiency virus biological phenotypes isolated from cerebrospinal fluid and peripheral blood
Quantitative human immunodeficiency virus (HIV) cultures were carried out on cerebrospinal fluid (CSF), peripheral blood mononuclear cells (PBMCs), and plasma from patients with HIV in order to compare the infectious HIV load. The HIV strains isolated were studied for syncytiuminducing (SI) capacity...
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| Veröffentlicht in: | Journal of medical virology 1995-09, Vol.47 (1), p.92-96 |
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| container_title | Journal of medical virology |
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| creator | Peeters, Martine F. Colebunders, Robert L. Van Den Abbeele, Koen Nys, Patrick C. Goeman, Johan Colans, Philippe Geerts, Yvette Van der Groen, Guido |
| description | Quantitative human immunodeficiency virus (HIV) cultures were carried out on cerebrospinal fluid (CSF), peripheral blood mononuclear cells (PBMCs), and plasma from patients with HIV in order to compare the infectious HIV load. The HIV strains isolated were studied for syncytiuminducing (SI) capacity, using the MT‐2 cell line, in order to compare the HIV strain phenotype of blood and CSF isolates.
Forty‐two patients with HIV‐1 infection were enrolled in the study, 33 of whom had neurological symptoms and 9 of whom were without neurological symptoms. HIV was isolated from 16 (38%) of the 42 CSF cultures, with a low mean titer of 6.3 ± 3.4 tissue‐culture‐infective doses (TCID) per milliliter. Patients with HIV‐positive CSF culture had a viral load in PBMCs of 40.5 ± 15.5 TCID per 106 PBMC and in plasma of 104.7± 9.3 per milliliter. Two (15%) of the 13 CSF isolates were Sl strains, compared to 17 (56.6%) of the 30 PBMC isolates and 13 (54%) of the 24 plasma isolates (P |
| doi_str_mv | 10.1002/jmv.1890470117 |
| format | Article |
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Forty‐two patients with HIV‐1 infection were enrolled in the study, 33 of whom had neurological symptoms and 9 of whom were without neurological symptoms. HIV was isolated from 16 (38%) of the 42 CSF cultures, with a low mean titer of 6.3 ± 3.4 tissue‐culture‐infective doses (TCID) per milliliter. Patients with HIV‐positive CSF culture had a viral load in PBMCs of 40.5 ± 15.5 TCID per 106 PBMC and in plasma of 104.7± 9.3 per milliliter. Two (15%) of the 13 CSF isolates were Sl strains, compared to 17 (56.6%) of the 30 PBMC isolates and 13 (54%) of the 24 plasma isolates (P<0.05). Five of the nine patients from whom CSF and blood strains were obtained had the same viral biological phenotype.
This study suggests that different HIV variants may be found in different body fluids and/or cells. © 1995 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.1890470117</identifier><identifier>PMID: 8551266</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; AIDS/HIV ; Biological and medical sciences ; Cells, Cultured ; cerebrospinal fluid ; Female ; Giant Cells - virology ; HIV ; HIV Infections - blood ; HIV Infections - cerebrospinal fluid ; HIV Infections - virology ; HIV-1 - isolation & purification ; HIV-1 - physiology ; human immunodeficiency virus ; Humans ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Leukocytes, Mononuclear - virology ; Male ; Medical sciences ; Neurologic Examination ; Phenotype ; viral load ; viral phenotype</subject><ispartof>Journal of medical virology, 1995-09, Vol.47 (1), p.92-96</ispartof><rights>Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-e9fb98971b43bd278b162907301d8c2dedb644ed7acc603e1e8d0b633f2408c03</citedby><cites>FETCH-LOGICAL-c3537-e9fb98971b43bd278b162907301d8c2dedb644ed7acc603e1e8d0b633f2408c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.1890470117$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.1890470117$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3644269$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8551266$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peeters, Martine F.</creatorcontrib><creatorcontrib>Colebunders, Robert L.</creatorcontrib><creatorcontrib>Van Den Abbeele, Koen</creatorcontrib><creatorcontrib>Nys, Patrick C.</creatorcontrib><creatorcontrib>Goeman, Johan</creatorcontrib><creatorcontrib>Colans, Philippe</creatorcontrib><creatorcontrib>Geerts, Yvette</creatorcontrib><creatorcontrib>Van der Groen, Guido</creatorcontrib><title>Comparison of human immunodeficiency virus biological phenotypes isolated from cerebrospinal fluid and peripheral blood</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Quantitative human immunodeficiency virus (HIV) cultures were carried out on cerebrospinal fluid (CSF), peripheral blood mononuclear cells (PBMCs), and plasma from patients with HIV in order to compare the infectious HIV load. The HIV strains isolated were studied for syncytiuminducing (SI) capacity, using the MT‐2 cell line, in order to compare the HIV strain phenotype of blood and CSF isolates.
Forty‐two patients with HIV‐1 infection were enrolled in the study, 33 of whom had neurological symptoms and 9 of whom were without neurological symptoms. HIV was isolated from 16 (38%) of the 42 CSF cultures, with a low mean titer of 6.3 ± 3.4 tissue‐culture‐infective doses (TCID) per milliliter. Patients with HIV‐positive CSF culture had a viral load in PBMCs of 40.5 ± 15.5 TCID per 106 PBMC and in plasma of 104.7± 9.3 per milliliter. Two (15%) of the 13 CSF isolates were Sl strains, compared to 17 (56.6%) of the 30 PBMC isolates and 13 (54%) of the 24 plasma isolates (P<0.05). Five of the nine patients from whom CSF and blood strains were obtained had the same viral biological phenotype.
This study suggests that different HIV variants may be found in different body fluids and/or cells. © 1995 Wiley‐Liss, Inc.</description><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>cerebrospinal fluid</subject><subject>Female</subject><subject>Giant Cells - virology</subject><subject>HIV</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - cerebrospinal fluid</subject><subject>HIV Infections - virology</subject><subject>HIV-1 - isolation & purification</subject><subject>HIV-1 - physiology</subject><subject>human immunodeficiency virus</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Leukocytes, Mononuclear - virology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neurologic Examination</subject><subject>Phenotype</subject><subject>viral load</subject><subject>viral phenotype</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKkNhyw7JC8Qugx-JHS_RFAb6AAnx2ll-3FCXJA72pGX-fV3NaFBXXVny_c49Pj4IvaRkSQlhb6-G6yVtFakloVQ-QgtKlKgUkfQxWhBai0oI2jxFz3K-IoS0irEjdNQ2DWVCLNDNKg6TSSHHEccOX86DGXEYhnmMHrrgAoxui69DmjO2Ifbxd3Cmx9MljHGznSDjIu3NBjzuUhywgwQ2xTyFsWBdPwePzejxBCkUUSqXto_RP0dPOtNneLE_j9H3D--_rT5W51_Wn1bvzivHGy4rUJ1VrZLU1tx6JltLBSvhOKG-dcyDt6KuwUvjnCAcKLSeWMF5x2rSOsKP0Zvd3inFvzPkjR5CdtD3ZoQ4Zy2lKEZcPghS0UrFqCrgcge6EjMn6PSUwmDSVlOi7yrRpRL9v5IieLXfPNsB_AHfd1Dmr_dzk8vfdsmMLuQDxktAJu581Q67CT1sHzDVpxc_7j2h2mlD3sC_g9akP1pILhv98_Nan9IT8vVMrfUvfgsMorZk</recordid><startdate>199509</startdate><enddate>199509</enddate><creator>Peeters, Martine F.</creator><creator>Colebunders, Robert L.</creator><creator>Van Den Abbeele, Koen</creator><creator>Nys, Patrick C.</creator><creator>Goeman, Johan</creator><creator>Colans, Philippe</creator><creator>Geerts, Yvette</creator><creator>Van der Groen, Guido</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199509</creationdate><title>Comparison of human immunodeficiency virus biological phenotypes isolated from cerebrospinal fluid and peripheral blood</title><author>Peeters, Martine F. ; Colebunders, Robert L. ; Van Den Abbeele, Koen ; Nys, Patrick C. ; Goeman, Johan ; Colans, Philippe ; Geerts, Yvette ; Van der Groen, Guido</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-e9fb98971b43bd278b162907301d8c2dedb644ed7acc603e1e8d0b633f2408c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adult</topic><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>cerebrospinal fluid</topic><topic>Female</topic><topic>Giant Cells - virology</topic><topic>HIV</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - cerebrospinal fluid</topic><topic>HIV Infections - virology</topic><topic>HIV-1 - isolation & purification</topic><topic>HIV-1 - physiology</topic><topic>human immunodeficiency virus</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Leukocytes, Mononuclear - virology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neurologic Examination</topic><topic>Phenotype</topic><topic>viral load</topic><topic>viral phenotype</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peeters, Martine F.</creatorcontrib><creatorcontrib>Colebunders, Robert L.</creatorcontrib><creatorcontrib>Van Den Abbeele, Koen</creatorcontrib><creatorcontrib>Nys, Patrick C.</creatorcontrib><creatorcontrib>Goeman, Johan</creatorcontrib><creatorcontrib>Colans, Philippe</creatorcontrib><creatorcontrib>Geerts, Yvette</creatorcontrib><creatorcontrib>Van der Groen, Guido</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peeters, Martine F.</au><au>Colebunders, Robert L.</au><au>Van Den Abbeele, Koen</au><au>Nys, Patrick C.</au><au>Goeman, Johan</au><au>Colans, Philippe</au><au>Geerts, Yvette</au><au>Van der Groen, Guido</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of human immunodeficiency virus biological phenotypes isolated from cerebrospinal fluid and peripheral blood</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>1995-09</date><risdate>1995</risdate><volume>47</volume><issue>1</issue><spage>92</spage><epage>96</epage><pages>92-96</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>Quantitative human immunodeficiency virus (HIV) cultures were carried out on cerebrospinal fluid (CSF), peripheral blood mononuclear cells (PBMCs), and plasma from patients with HIV in order to compare the infectious HIV load. The HIV strains isolated were studied for syncytiuminducing (SI) capacity, using the MT‐2 cell line, in order to compare the HIV strain phenotype of blood and CSF isolates.
Forty‐two patients with HIV‐1 infection were enrolled in the study, 33 of whom had neurological symptoms and 9 of whom were without neurological symptoms. HIV was isolated from 16 (38%) of the 42 CSF cultures, with a low mean titer of 6.3 ± 3.4 tissue‐culture‐infective doses (TCID) per milliliter. Patients with HIV‐positive CSF culture had a viral load in PBMCs of 40.5 ± 15.5 TCID per 106 PBMC and in plasma of 104.7± 9.3 per milliliter. Two (15%) of the 13 CSF isolates were Sl strains, compared to 17 (56.6%) of the 30 PBMC isolates and 13 (54%) of the 24 plasma isolates (P<0.05). Five of the nine patients from whom CSF and blood strains were obtained had the same viral biological phenotype.
This study suggests that different HIV variants may be found in different body fluids and/or cells. © 1995 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8551266</pmid><doi>10.1002/jmv.1890470117</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of medical virology, 1995-09, Vol.47 (1), p.92-96 |
| issn | 0146-6615 1096-9071 |
| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Adult AIDS/HIV Biological and medical sciences Cells, Cultured cerebrospinal fluid Female Giant Cells - virology HIV HIV Infections - blood HIV Infections - cerebrospinal fluid HIV Infections - virology HIV-1 - isolation & purification HIV-1 - physiology human immunodeficiency virus Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Leukocytes, Mononuclear - virology Male Medical sciences Neurologic Examination Phenotype viral load viral phenotype |
| title | Comparison of human immunodeficiency virus biological phenotypes isolated from cerebrospinal fluid and peripheral blood |
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