PAF effects on eicosanoid release in neonatal rat cardiomyocytes

PAF effects on eicosanoid release in neonatal rat cardiomyocytes

The aim of this study was to find out whether, in neonatal rat cardiomyocytes, platelet-activating factor (PAF) can stimulate eicosanoid formation. For this purpose neonatal cardiomyocytes were incubated for 60 min at 37°C in HANKS buffer with PAF (10–1000 nM), and the eicosanoids thromboxane A 2 (T...

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Veröffentlicht in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 1995-09, Vol.53 (3), p.197-200
Hauptverfasser: Becker, K., Heinroth-Hoffmann, I., Giessler, Ch, Pönicke, K., Brodde, O.-E.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Animals, Newborn
Aspirin - pharmacology
Azepines - pharmacology
Biological and medical sciences
Cyclooxygenase Inhibitors - pharmacology
Epoprostenol - metabolism
Fundamental and applied biological sciences. Psychology
Heart
Heart - physiology
Platelet Activating Factor - antagonists & inhibitors
Platelet Activating Factor - pharmacology
Platelet Aggregation Inhibitors - pharmacology
Rats
Rats, Wistar
Thromboxane A2 - metabolism
Triazoles - pharmacology
Vertebrates: cardiovascular system
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Zusammenfassung:The aim of this study was to find out whether, in neonatal rat cardiomyocytes, platelet-activating factor (PAF) can stimulate eicosanoid formation. For this purpose neonatal cardiomyocytes were incubated for 60 min at 37°C in HANKS buffer with PAF (10–1000 nM), and the eicosanoids thromboxane A 2 (TXA 2) and prostacyclin (PGI 2) were assessed in the supernatant as TXB 2 and 6-keto-PGF 1α, respectively, by an enzyme immunoassay. PAF caused concentration-dependent release of PGI 2; TXA 2, however, was significantly released only at the highest concentration of PAF (1000 nM). Acetylsalicylic acid (556 μM) and the PAF antagonist WEB 2086 (10 μM) significantly attenuated PAF-induced eicosanoid formation. We conclude that in neonatal rat cardiomyocytes PAF can induce eicosanoid formation and this effect is brought about by activation of a specific PAF receptor.
ISSN:0952-3278
1532-2823
DOI:10.1016/0952-3278(95)90116-7