Substance P depresses neuronal activity in the rat olfactory bulb in vitro and in vivo: possible mediation via γ-aminobutyric acid release
The rat olfactory bulb is an area displaying a particularly high density of substance P receptors in the glomerular cell layer whose functions are unknown. In pilot in vivo experiments we discovered that iontoporetically administered substance P potently depressed the spontaneous firing rate of most...
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| Veröffentlicht in: | Brain research 1987-06, Vol.412 (2), p.269-274 |
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| creator | Olpe, H.R. Heid, J. Bittiger, H. Steinmann, M.W. |
| description | The rat olfactory bulb is an area displaying a particularly high density of substance P receptors in the glomerular cell layer whose functions are unknown. In pilot in vivo experiments we discovered that iontoporetically administered substance P potently depressed the spontaneous firing rate of most unidentified neurons of the rat olfactory bulb. To further elucidate the mechanism of this unexpected depressant effect, we studied the peptide's action in vitro on coronal sections of this brain region. Bath applied and microiontophoretically administered substance P depressed the spontaneous discharge of unidentified glomerular neurons in a dose-dependent fashion. This inhibiting effect is mediated indirectly via the release of another transmitter because it was abolished completely if the standard perfusion medium was replaced by a medium containing zero calcium and high magnesium. It appears that substance P acts by means of releasing GABA which in turn evokes the observed cell depression because the depressant effects were completely abolished by bath-applied bicuculline (10 μM) and picrotoxin (100 μM). In conclusion we propose that substance P indirectly depresses neuronal activity in the glomerular cell layer of the rat olfactory bulb by releasing γ-aminobutyric acid. |
| doi_str_mv | 10.1016/0006-8993(87)91133-4 |
| format | Article |
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In pilot in vivo experiments we discovered that iontoporetically administered substance P potently depressed the spontaneous firing rate of most unidentified neurons of the rat olfactory bulb. To further elucidate the mechanism of this unexpected depressant effect, we studied the peptide's action in vitro on coronal sections of this brain region. Bath applied and microiontophoretically administered substance P depressed the spontaneous discharge of unidentified glomerular neurons in a dose-dependent fashion. This inhibiting effect is mediated indirectly via the release of another transmitter because it was abolished completely if the standard perfusion medium was replaced by a medium containing zero calcium and high magnesium. It appears that substance P acts by means of releasing GABA which in turn evokes the observed cell depression because the depressant effects were completely abolished by bath-applied bicuculline (10 μM) and picrotoxin (100 μM). In conclusion we propose that substance P indirectly depresses neuronal activity in the glomerular cell layer of the rat olfactory bulb by releasing γ-aminobutyric acid.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(87)91133-4</identifier><identifier>PMID: 2440522</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Action Potentials - drug effects ; Animals ; Bicuculline - pharmacology ; Biological and medical sciences ; Electrophysiology ; Fundamental and applied biological sciences. Psychology ; gamma-Aminobutyric Acid - metabolism ; Glomerular cell layer ; In Vitro Techniques ; Iontophoresis ; Male ; Neurons - drug effects ; Neurons - physiology ; Olfactory bulb ; Olfactory Bulb - drug effects ; Olfactory Bulb - physiology ; Olfactory system and olfaction. 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In pilot in vivo experiments we discovered that iontoporetically administered substance P potently depressed the spontaneous firing rate of most unidentified neurons of the rat olfactory bulb. To further elucidate the mechanism of this unexpected depressant effect, we studied the peptide's action in vitro on coronal sections of this brain region. Bath applied and microiontophoretically administered substance P depressed the spontaneous discharge of unidentified glomerular neurons in a dose-dependent fashion. This inhibiting effect is mediated indirectly via the release of another transmitter because it was abolished completely if the standard perfusion medium was replaced by a medium containing zero calcium and high magnesium. It appears that substance P acts by means of releasing GABA which in turn evokes the observed cell depression because the depressant effects were completely abolished by bath-applied bicuculline (10 μM) and picrotoxin (100 μM). In conclusion we propose that substance P indirectly depresses neuronal activity in the glomerular cell layer of the rat olfactory bulb by releasing γ-aminobutyric acid.</description><subject>Action Potentials - drug effects</subject><subject>Animals</subject><subject>Bicuculline - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Glomerular cell layer</subject><subject>In Vitro Techniques</subject><subject>Iontophoresis</subject><subject>Male</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Olfactory bulb</subject><subject>Olfactory Bulb - drug effects</subject><subject>Olfactory Bulb - physiology</subject><subject>Olfactory system and olfaction. Gustatory system and gustation</subject><subject>Rats</subject><subject>Slice</subject><subject>Substance P</subject><subject>Substance P - pharmacology</subject><subject>Substance P - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>γ-Aminobutyric acid (GABA)</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuKFDEUhgtRxp7RN1DIQkQXpblVLi4EGcYLDCio65CkTjBSXWmTVEM_g4_je_hMpuyml7oKJ_93Dsn5uu4RwS8IJuIlxlj0Smv2TMnnmhDGen6n2xAlaS8ox3e7zRm5312W8r2VjGl80V1QzvFA6ab7-XlxpdrZA_qERthlKAUKmmHJabYTsr7GfawHFGdUvwHKtqI0hXad8gG5ZXJr0oickJ3HY7FPr9AulRLdBGgLY7Q1pjWw6Pev3m7jnNxSDzn6Nj-OKMMEtsCD7l6wU4GHp_Oq-_r25sv1-_7247sP129ue88Yrb1kjgpuhQrED0C8Cp6AG7HEDAuvxUjAUx8cCB7EMDDpAgitROBCezo6dtU9Pc7d5fRjgVLNNhYP02RnSEsxUgqqMRn-CxKuMFdSN5AfQZ_btzMEs8txa_PBEGxWWWY1YVYTRknzV5bhre3xaf7i2prOTSc7LX9yym3xdgq5eYrljClGhdayYa-PGLSl7SNkU3yEpnSMGXw1Y4r_fscf3VCzZQ</recordid><startdate>19870602</startdate><enddate>19870602</enddate><creator>Olpe, H.R.</creator><creator>Heid, J.</creator><creator>Bittiger, H.</creator><creator>Steinmann, M.W.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19870602</creationdate><title>Substance P depresses neuronal activity in the rat olfactory bulb in vitro and in vivo: possible mediation via γ-aminobutyric acid release</title><author>Olpe, H.R. ; Heid, J. ; Bittiger, H. ; Steinmann, M.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-73b264a68f1c5e1c8fc1ebd070306c96d1ec2cfbe64f65537bfe6986f469c2db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Action Potentials - drug effects</topic><topic>Animals</topic><topic>Bicuculline - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Electrophysiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Glomerular cell layer</topic><topic>In Vitro Techniques</topic><topic>Iontophoresis</topic><topic>Male</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Olfactory bulb</topic><topic>Olfactory Bulb - drug effects</topic><topic>Olfactory Bulb - physiology</topic><topic>Olfactory system and olfaction. Gustatory system and gustation</topic><topic>Rats</topic><topic>Slice</topic><topic>Substance P</topic><topic>Substance P - pharmacology</topic><topic>Substance P - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>γ-Aminobutyric acid (GABA)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olpe, H.R.</creatorcontrib><creatorcontrib>Heid, J.</creatorcontrib><creatorcontrib>Bittiger, H.</creatorcontrib><creatorcontrib>Steinmann, M.W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olpe, H.R.</au><au>Heid, J.</au><au>Bittiger, H.</au><au>Steinmann, M.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Substance P depresses neuronal activity in the rat olfactory bulb in vitro and in vivo: possible mediation via γ-aminobutyric acid release</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1987-06-02</date><risdate>1987</risdate><volume>412</volume><issue>2</issue><spage>269</spage><epage>274</epage><pages>269-274</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The rat olfactory bulb is an area displaying a particularly high density of substance P receptors in the glomerular cell layer whose functions are unknown. In pilot in vivo experiments we discovered that iontoporetically administered substance P potently depressed the spontaneous firing rate of most unidentified neurons of the rat olfactory bulb. To further elucidate the mechanism of this unexpected depressant effect, we studied the peptide's action in vitro on coronal sections of this brain region. Bath applied and microiontophoretically administered substance P depressed the spontaneous discharge of unidentified glomerular neurons in a dose-dependent fashion. This inhibiting effect is mediated indirectly via the release of another transmitter because it was abolished completely if the standard perfusion medium was replaced by a medium containing zero calcium and high magnesium. It appears that substance P acts by means of releasing GABA which in turn evokes the observed cell depression because the depressant effects were completely abolished by bath-applied bicuculline (10 μM) and picrotoxin (100 μM). In conclusion we propose that substance P indirectly depresses neuronal activity in the glomerular cell layer of the rat olfactory bulb by releasing γ-aminobutyric acid.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2440522</pmid><doi>10.1016/0006-8993(87)91133-4</doi><tpages>6</tpages></addata></record> |
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| subjects | Action Potentials - drug effects Animals Bicuculline - pharmacology Biological and medical sciences Electrophysiology Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - metabolism Glomerular cell layer In Vitro Techniques Iontophoresis Male Neurons - drug effects Neurons - physiology Olfactory bulb Olfactory Bulb - drug effects Olfactory Bulb - physiology Olfactory system and olfaction. Gustatory system and gustation Rats Slice Substance P Substance P - pharmacology Substance P - physiology Vertebrates: nervous system and sense organs γ-Aminobutyric acid (GABA) |
| title | Substance P depresses neuronal activity in the rat olfactory bulb in vitro and in vivo: possible mediation via γ-aminobutyric acid release |
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