An optimized thymidylate kinase assay, based on enzymatically synthesized 5-[ 125I]iododeoxyuridine monophosphate and its application to an immunological study of herpes simplex virus thymidine-thymidylate kinases

The biological synthesis and purification of 5-[ 125I]iododeoxyuridine monophosphate (IdUMP) are described. The specificity of IdUMP as substrate in the thymidylate monophosphate kinase (TMPK) assay is demonstrated, and a 100-fold gain in sensitivity as compared to the conventional TMPK assay is sho...

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Veröffentlicht in:	Anal. Biochem.; (United States) 1987-05, Vol.162 (2), p.500-510
Hauptverfasser:	Karlström, Anders R., Gronowitz, J.Simon
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques Adenosine Triphosphate Animals ANTIBODIES Antibodies - analysis BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BIOSYNTHESIS Brain Neoplasms - enzymology CATALYSIS Cell Line CHEMICAL REACTIONS DAYS LIVING RADIOISOTOPES Deoxyuracil Nucleotides - biosynthesis DISEASES ELECTRON CAPTURE RADIOISOTOPES ENZYME ACTIVITY enzyme activity blocking antibody ENZYME IMMUNOASSAY ENZYMES FRACTIONATION HERPES SIMPLEX herpes simplex virus Humans Hydrogen-Ion Concentration IMMUNOASSAY INFECTIOUS DISEASES INHIBITION INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES Iodine Radioisotopes iododeoxyuridine monophosphate ISOTOPE APPLICATIONS ISOTOPES NUCLEI Nucleoside-Phosphate Kinase - analysis Nucleoside-Phosphate Kinase - immunology NUCLEOTIDES ODD-EVEN NUCLEI ORGANIC COMPOUNDS PHOSPHORUS-GROUP TRANSFERASES PHOSPHORYLATION PHOSPHOTRANSFERASES Phosphotransferases - analysis RADIOISOTOPES SEPARATION PROCESSES Simplexvirus - enzymology SKIN DISEASES SYNTHESIS thymidine kinase Thymidine Kinase - analysis Thymidine Kinase - immunology thymidylate kinase assay TRACER TECHNIQUES TRANSFERASES UMP VIRAL DISEASES
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