A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques
ZAVOS, P.M., CORREA, J.R., SOFIKITIS, N., KOFINAS, G.D. and ZARMAKOUPIS, P.N. A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques. Tohoku J. Exp. Med., 1995, 176 (2), 75-81 - The objective of this study was to determine if spermato...
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| Veröffentlicht in: | The Tohoku Journal of Experimental Medicine 1995, Vol.176(2), pp.75-81 |
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| container_title | The Tohoku Journal of Experimental Medicine |
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| creator | ZAVOS, PANAYIOTIS M. CORREA, JUAN B. SOFIKITIS, NIKOLAS KOFINAS, GEORGE D. ZARMAKOUPIS, PANAYOTA N. |
| description | ZAVOS, P.M., CORREA, J.R., SOFIKITIS, N., KOFINAS, G.D. and ZARMAKOUPIS, P.N. A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques. Tohoku J. Exp. Med., 1995, 176 (2), 75-81 - The objective of this study was to determine if spermatozoa, following short-term cyostorage at 5°C in Test-Yolk buffer (TYB; ZBL, Inc., Lexington, KY, USA), could be recovered and improved via the SpermPrepTM filtration method and to assess the possibly enhanced fertilizing capacity of the selected spermatozoa. Semen specimens from 20 men were collected, evaluated, diluted 1:1 (v/v) with TYB, divided into aliquots and cooled to 5°C for 24 and 48hr. Semen samples were assessed for volume, sperm count, percentage and grade of motility, percentage of morphologically normal spermatozoa and outcome of the sperm penetration assay (SPA). After storage, aliquots were rewarmed at 37°C, centrifuged, and the pellet was resuspended in 1.0ml of SpermPrepTM media (ZBL, Inc.). Following 15min of incubation, the rewarmed spermatozoa were filtered via the SpermPrepTM I filtration column (ZBL, Inc.) and assessed accordingly. The results obtained in this study indicate that the short-term cryostorage procedure yielded spermatozoa of adequate qualitative characteristics when compared to the fresh spermatozoa. Furthermore, filtration of rewarmed specimens yielded spermatozoa of significantly higher qualitative characteristics and superior fertilizing capacity following a short-term cyostorage period in TYB when compared to fresh and rewarmed spermatozoa (p |
| doi_str_mv | 10.1620/tjem.176.75 |
| format | Article |
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A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques. Tohoku J. Exp. Med., 1995, 176 (2), 75-81 - The objective of this study was to determine if spermatozoa, following short-term cyostorage at 5°C in Test-Yolk buffer (TYB; ZBL, Inc., Lexington, KY, USA), could be recovered and improved via the SpermPrepTM filtration method and to assess the possibly enhanced fertilizing capacity of the selected spermatozoa. Semen specimens from 20 men were collected, evaluated, diluted 1:1 (v/v) with TYB, divided into aliquots and cooled to 5°C for 24 and 48hr. Semen samples were assessed for volume, sperm count, percentage and grade of motility, percentage of morphologically normal spermatozoa and outcome of the sperm penetration assay (SPA). After storage, aliquots were rewarmed at 37°C, centrifuged, and the pellet was resuspended in 1.0ml of SpermPrepTM media (ZBL, Inc.). Following 15min of incubation, the rewarmed spermatozoa were filtered via the SpermPrepTM I filtration column (ZBL, Inc.) and assessed accordingly. The results obtained in this study indicate that the short-term cryostorage procedure yielded spermatozoa of adequate qualitative characteristics when compared to the fresh spermatozoa. Furthermore, filtration of rewarmed specimens yielded spermatozoa of significantly higher qualitative characteristics and superior fertilizing capacity following a short-term cyostorage period in TYB when compared to fresh and rewarmed spermatozoa (p<0.05). This method of short-term cyostorage in TYB and selection of superior spermatozoa via the SpermPrepTM filtration method could further enhance the fertilizing ability of patients who produce spermatozoa characterized by deficient capacitation, acrosome reaction and subsequent fertilization.</description><identifier>ISSN: 0040-8727</identifier><identifier>EISSN: 1349-3329</identifier><identifier>DOI: 10.1620/tjem.176.75</identifier><identifier>PMID: 7482525</identifier><language>eng</language><publisher>Japan: Tohoku University Medical Press</publisher><subject>Cryopreservation - methods ; cryopreserved spermatozoa ; Female ; Humans ; Male ; semen filtration ; Semen Preservation - methods ; Sperm Motility - physiology ; sperm selection ; Sperm-Ovum Interactions - physiology ; SpermPrepTM</subject><ispartof>The Tohoku Journal of Experimental Medicine, 1995, Vol.176(2), pp.75-81</ispartof><rights>Tohoku University Medical Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c579t-a643d25727bda609fe75e83941cfba357e221b512a909d2ff6a479b8c79734a83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7482525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ZAVOS, PANAYIOTIS M.</creatorcontrib><creatorcontrib>CORREA, JUAN B.</creatorcontrib><creatorcontrib>SOFIKITIS, NIKOLAS</creatorcontrib><creatorcontrib>KOFINAS, GEORGE D.</creatorcontrib><creatorcontrib>ZARMAKOUPIS, PANAYOTA N.</creatorcontrib><title>A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques</title><title>The Tohoku Journal of Experimental Medicine</title><addtitle>Tohoku J. Exp. Med.</addtitle><description>ZAVOS, P.M., CORREA, J.R., SOFIKITIS, N., KOFINAS, G.D. and ZARMAKOUPIS, P.N. A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques. Tohoku J. Exp. Med., 1995, 176 (2), 75-81 - The objective of this study was to determine if spermatozoa, following short-term cyostorage at 5°C in Test-Yolk buffer (TYB; ZBL, Inc., Lexington, KY, USA), could be recovered and improved via the SpermPrepTM filtration method and to assess the possibly enhanced fertilizing capacity of the selected spermatozoa. Semen specimens from 20 men were collected, evaluated, diluted 1:1 (v/v) with TYB, divided into aliquots and cooled to 5°C for 24 and 48hr. Semen samples were assessed for volume, sperm count, percentage and grade of motility, percentage of morphologically normal spermatozoa and outcome of the sperm penetration assay (SPA). After storage, aliquots were rewarmed at 37°C, centrifuged, and the pellet was resuspended in 1.0ml of SpermPrepTM media (ZBL, Inc.). Following 15min of incubation, the rewarmed spermatozoa were filtered via the SpermPrepTM I filtration column (ZBL, Inc.) and assessed accordingly. The results obtained in this study indicate that the short-term cryostorage procedure yielded spermatozoa of adequate qualitative characteristics when compared to the fresh spermatozoa. Furthermore, filtration of rewarmed specimens yielded spermatozoa of significantly higher qualitative characteristics and superior fertilizing capacity following a short-term cyostorage period in TYB when compared to fresh and rewarmed spermatozoa (p<0.05). This method of short-term cyostorage in TYB and selection of superior spermatozoa via the SpermPrepTM filtration method could further enhance the fertilizing ability of patients who produce spermatozoa characterized by deficient capacitation, acrosome reaction and subsequent fertilization.</description><subject>Cryopreservation - methods</subject><subject>cryopreserved spermatozoa</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>semen filtration</subject><subject>Semen Preservation - methods</subject><subject>Sperm Motility - physiology</subject><subject>sperm selection</subject><subject>Sperm-Ovum Interactions - physiology</subject><subject>SpermPrepTM</subject><issn>0040-8727</issn><issn>1349-3329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM9v2yAYhtG0qcvSnnaexGmXySk_jDHHKGq3SZ0mLWmvCOPPM5FtMiDV-t8XN1EucHgfXr7vQegzJStaMXKb9jCuqKxWUrxDC8pLVXDO1Hu0IKQkRS2Z_Ig-xbgnhJdEVlfoSpY1E0ws0P81_gWp9y32Hd72PqRiB2HEm_DiY_LB_AVsphZvYQCbnJ9m7smZZgC8Pcxk5wN-jIDdhFMP-MkE548Rr2N0MUGL_8Ah-PaYHz8D3oHtJ_fvCPEafejMEOHmfC_R4_3dbvOjePj9_edm_VBYIVUqTFXylom8QtOaiqgOpICaq5LarjFcSGCMNoIyo4hqWddVppSqqa1Ukpem5kv09dSbp5j_TXp00cIwmAnynFrKitE6w0v07QTa4GMM0OlDcKMJL5oSPXvWs2edPWspMv3lXHtsRmgv7Flszu9O-T6m7PCSm5CcHeCti6pcOvex8ynFJbe9CRom_grB1pJe</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>ZAVOS, PANAYIOTIS M.</creator><creator>CORREA, JUAN B.</creator><creator>SOFIKITIS, NIKOLAS</creator><creator>KOFINAS, GEORGE D.</creator><creator>ZARMAKOUPIS, PANAYOTA N.</creator><general>Tohoku University Medical Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques</title><author>ZAVOS, PANAYIOTIS M. ; CORREA, JUAN B. ; SOFIKITIS, NIKOLAS ; KOFINAS, GEORGE D. ; ZARMAKOUPIS, PANAYOTA N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c579t-a643d25727bda609fe75e83941cfba357e221b512a909d2ff6a479b8c79734a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Cryopreservation - methods</topic><topic>cryopreserved spermatozoa</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>semen filtration</topic><topic>Semen Preservation - methods</topic><topic>Sperm Motility - physiology</topic><topic>sperm selection</topic><topic>Sperm-Ovum Interactions - physiology</topic><topic>SpermPrepTM</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ZAVOS, PANAYIOTIS M.</creatorcontrib><creatorcontrib>CORREA, JUAN B.</creatorcontrib><creatorcontrib>SOFIKITIS, NIKOLAS</creatorcontrib><creatorcontrib>KOFINAS, GEORGE D.</creatorcontrib><creatorcontrib>ZARMAKOUPIS, PANAYOTA N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Tohoku Journal of Experimental Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZAVOS, PANAYIOTIS M.</au><au>CORREA, JUAN B.</au><au>SOFIKITIS, NIKOLAS</au><au>KOFINAS, GEORGE D.</au><au>ZARMAKOUPIS, PANAYOTA N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques</atitle><jtitle>The Tohoku Journal of Experimental Medicine</jtitle><addtitle>Tohoku J. Exp. Med.</addtitle><date>1995</date><risdate>1995</risdate><volume>176</volume><issue>2</issue><spage>75</spage><epage>81</epage><pages>75-81</pages><issn>0040-8727</issn><eissn>1349-3329</eissn><abstract>ZAVOS, P.M., CORREA, J.R., SOFIKITIS, N., KOFINAS, G.D. and ZARMAKOUPIS, P.N. A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques. Tohoku J. Exp. Med., 1995, 176 (2), 75-81 - The objective of this study was to determine if spermatozoa, following short-term cyostorage at 5°C in Test-Yolk buffer (TYB; ZBL, Inc., Lexington, KY, USA), could be recovered and improved via the SpermPrepTM filtration method and to assess the possibly enhanced fertilizing capacity of the selected spermatozoa. Semen specimens from 20 men were collected, evaluated, diluted 1:1 (v/v) with TYB, divided into aliquots and cooled to 5°C for 24 and 48hr. Semen samples were assessed for volume, sperm count, percentage and grade of motility, percentage of morphologically normal spermatozoa and outcome of the sperm penetration assay (SPA). After storage, aliquots were rewarmed at 37°C, centrifuged, and the pellet was resuspended in 1.0ml of SpermPrepTM media (ZBL, Inc.). Following 15min of incubation, the rewarmed spermatozoa were filtered via the SpermPrepTM I filtration column (ZBL, Inc.) and assessed accordingly. The results obtained in this study indicate that the short-term cryostorage procedure yielded spermatozoa of adequate qualitative characteristics when compared to the fresh spermatozoa. Furthermore, filtration of rewarmed specimens yielded spermatozoa of significantly higher qualitative characteristics and superior fertilizing capacity following a short-term cyostorage period in TYB when compared to fresh and rewarmed spermatozoa (p<0.05). This method of short-term cyostorage in TYB and selection of superior spermatozoa via the SpermPrepTM filtration method could further enhance the fertilizing ability of patients who produce spermatozoa characterized by deficient capacitation, acrosome reaction and subsequent fertilization.</abstract><cop>Japan</cop><pub>Tohoku University Medical Press</pub><pmid>7482525</pmid><doi>10.1620/tjem.176.75</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cryopreservation - methods cryopreserved spermatozoa Female Humans Male semen filtration Semen Preservation - methods Sperm Motility - physiology sperm selection Sperm-Ovum Interactions - physiology SpermPrepTM |
| title | A Method of Short-Term Cryostorage and Selection of Viable Sperm for Use in the Various Assisted Reproductive Techniques |
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