Isolation of several cDNAs encoding yeast peroxisomal enzymes
Several candidate clones carrying partial cDNAs for yeast peroxisomal enzymes, such as catalase, carnitine acetyltransferase, isocitrate lyase, malate synthase and acyl-CoA oxidase, were efficiently isolated at a single plating from a phage λgt11 recombinant cDNA library prepared with poly(A)-rich R...
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| Veröffentlicht in: | FEBS letters 1987-08, Vol.220 (1), p.31-35 |
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| container_title | FEBS letters |
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| creator | Ueda, Mitsuyoshi Okada, Hirofumi Hishida, Tadashi Teranishi, Yutaka Tanaka, Atsuo |
| description | Several candidate clones carrying partial cDNAs for yeast peroxisomal enzymes, such as catalase, carnitine acetyltransferase, isocitrate lyase, malate synthase and acyl-CoA oxidase, were efficiently isolated at a single plating from a phage λgt11 recombinant cDNA library prepared with poly(A)-rich RNA from an
n-alkane-grown yeast,
Candida tropicalis, with a mixture of antibodies against the respective purified enzymes. Among them, one candidate clone carrying partial cDNA for catalase was subcloned and subjected to nucleotide sequence analysis. We succeeded in determining that the amino acid sequence deduced from the nucleotide analysis included the sequences derived from the two peptide fragments obtained from the purified enzyme. |
| doi_str_mv | 10.1016/0014-5793(87)80870-0 |
| format | Article |
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n-alkane-grown yeast,
Candida tropicalis, with a mixture of antibodies against the respective purified enzymes. Among them, one candidate clone carrying partial cDNA for catalase was subcloned and subjected to nucleotide sequence analysis. We succeeded in determining that the amino acid sequence deduced from the nucleotide analysis included the sequences derived from the two peptide fragments obtained from the purified enzyme.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(87)80870-0</identifier><identifier>PMID: 2440725</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Alkane-utilizing yeast ; Amino Acid Sequence ; Autoradiography ; Biological and medical sciences ; Candida - enzymology ; Candida - genetics ; Candida tropicalis ; Catalase ; Catalase - genetics ; cDNA expression library ; DNA - isolation & purification ; DNA sequence ; DNA, Recombinant ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Microbodies - enzymology ; Molecular and cellular biology ; Molecular genetics ; Nucleic Acid Hybridization ; Nucleotides - analysis ; Peptide Fragments - analysis ; Peroxisomal enzyme ; RNA - analysis</subject><ispartof>FEBS letters, 1987-08, Vol.220 (1), p.31-35</ispartof><rights>1987</rights><rights>FEBS Letters 220 (1987) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5610-583911ee7003a53e14170cd9f17ffe837db16f9cd6ac369afdc0bcca81bc4c173</citedby><cites>FETCH-LOGICAL-c5610-583911ee7003a53e14170cd9f17ffe837db16f9cd6ac369afdc0bcca81bc4c173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(87)80870-0$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7642275$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2440725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ueda, Mitsuyoshi</creatorcontrib><creatorcontrib>Okada, Hirofumi</creatorcontrib><creatorcontrib>Hishida, Tadashi</creatorcontrib><creatorcontrib>Teranishi, Yutaka</creatorcontrib><creatorcontrib>Tanaka, Atsuo</creatorcontrib><title>Isolation of several cDNAs encoding yeast peroxisomal enzymes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Several candidate clones carrying partial cDNAs for yeast peroxisomal enzymes, such as catalase, carnitine acetyltransferase, isocitrate lyase, malate synthase and acyl-CoA oxidase, were efficiently isolated at a single plating from a phage λgt11 recombinant cDNA library prepared with poly(A)-rich RNA from an
n-alkane-grown yeast,
Candida tropicalis, with a mixture of antibodies against the respective purified enzymes. Among them, one candidate clone carrying partial cDNA for catalase was subcloned and subjected to nucleotide sequence analysis. We succeeded in determining that the amino acid sequence deduced from the nucleotide analysis included the sequences derived from the two peptide fragments obtained from the purified enzyme.</description><subject>Alkane-utilizing yeast</subject><subject>Amino Acid Sequence</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Candida - enzymology</subject><subject>Candida - genetics</subject><subject>Candida tropicalis</subject><subject>Catalase</subject><subject>Catalase - genetics</subject><subject>cDNA expression library</subject><subject>DNA - isolation & purification</subject><subject>DNA sequence</subject><subject>DNA, Recombinant</subject><subject>Fundamental and applied biological sciences. 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Genome</topic><topic>Microbodies - enzymology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Hybridization</topic><topic>Nucleotides - analysis</topic><topic>Peptide Fragments - analysis</topic><topic>Peroxisomal enzyme</topic><topic>RNA - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ueda, Mitsuyoshi</creatorcontrib><creatorcontrib>Okada, Hirofumi</creatorcontrib><creatorcontrib>Hishida, Tadashi</creatorcontrib><creatorcontrib>Teranishi, Yutaka</creatorcontrib><creatorcontrib>Tanaka, Atsuo</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ueda, Mitsuyoshi</au><au>Okada, Hirofumi</au><au>Hishida, Tadashi</au><au>Teranishi, Yutaka</au><au>Tanaka, Atsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of several cDNAs encoding yeast peroxisomal enzymes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1987-08-10</date><risdate>1987</risdate><volume>220</volume><issue>1</issue><spage>31</spage><epage>35</epage><pages>31-35</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Several candidate clones carrying partial cDNAs for yeast peroxisomal enzymes, such as catalase, carnitine acetyltransferase, isocitrate lyase, malate synthase and acyl-CoA oxidase, were efficiently isolated at a single plating from a phage λgt11 recombinant cDNA library prepared with poly(A)-rich RNA from an
n-alkane-grown yeast,
Candida tropicalis, with a mixture of antibodies against the respective purified enzymes. Among them, one candidate clone carrying partial cDNA for catalase was subcloned and subjected to nucleotide sequence analysis. We succeeded in determining that the amino acid sequence deduced from the nucleotide analysis included the sequences derived from the two peptide fragments obtained from the purified enzyme.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2440725</pmid><doi>10.1016/0014-5793(87)80870-0</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1987-08, Vol.220 (1), p.31-35 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Alkane-utilizing yeast Amino Acid Sequence Autoradiography Biological and medical sciences Candida - enzymology Candida - genetics Candida tropicalis Catalase Catalase - genetics cDNA expression library DNA - isolation & purification DNA sequence DNA, Recombinant Fundamental and applied biological sciences. Psychology Genes. Genome Microbodies - enzymology Molecular and cellular biology Molecular genetics Nucleic Acid Hybridization Nucleotides - analysis Peptide Fragments - analysis Peroxisomal enzyme RNA - analysis |
| title | Isolation of several cDNAs encoding yeast peroxisomal enzymes |
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