Human periodontal ligament and gingival fibroblast response to TGF-beta 1 stimulation
The purpose of this study was to measure the time-sequence response of RNA and protein synthesis to transforming growth factor-beta 1 (TGF-beta 1) by human periodontal ligament (HPDLF) and gingival (HGF) fibroblasts in culture. HPDLF and HGF were cultured from explants of healthy gingival tissue and...
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| Veröffentlicht in: | Journal of clinical periodontology 1995-09, Vol.22 (9), p.679-685 |
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| container_title | Journal of clinical periodontology |
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| creator | Mailhot, J M Schuster, G S Garnick, J J Hanes, P J Lapp, C A Lewis, J B |
| description | The purpose of this study was to measure the time-sequence response of RNA and protein synthesis to transforming growth factor-beta 1 (TGF-beta 1) by human periodontal ligament (HPDLF) and gingival (HGF) fibroblasts in culture. HPDLF and HGF were cultured from explants of healthy gingival tissue and freshly extracted teeth. Cultures of 8 x 10(4) cells/ml were exposed to medium containing 3H-uridine and 35S-methionine with TGF-beta 1 at concentrations from 10(-9) M to 10(-21) M, or control medium, for up to 60 hours in order to assess RNA and protein synthesis. Protein concentrations of comparable cultures were also assayed colorimetrically. Results were reported as specific activity (CPM/microgram protein). The results indicate that 10(-9) M TGF-beta 1 treated cultures showed a significant increase in RNA synthesis by HPDLF and HGF over time, as compared to the control cultures. HPDLF showed a significant increase in protein synthesis over time while that by HGF was not significant as compared to the control cultures. Lower concentrations of TGF-beta 1 demonstrated no significant differences from control. Results suggest that the effects of TGF-beta 1 on HPDLF and HGF are both time and dose dependent, with 10(-9) M TGF-beta 1 providing the best response of those concentrations tested. These findings support the concept that TGF-beta 1 may play a role in periodontal regeneration due to its ability to promote fibroblast RNA and protein synthesis. The results also demonstrate that although these two cells types appear morphologically similar, they exhibit distinct biological responses to growth factors such as TGF-beta 1. |
| doi_str_mv | 10.1111/j.1600-051X.1995.tb00826.x |
| format | Article |
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HPDLF and HGF were cultured from explants of healthy gingival tissue and freshly extracted teeth. Cultures of 8 x 10(4) cells/ml were exposed to medium containing 3H-uridine and 35S-methionine with TGF-beta 1 at concentrations from 10(-9) M to 10(-21) M, or control medium, for up to 60 hours in order to assess RNA and protein synthesis. Protein concentrations of comparable cultures were also assayed colorimetrically. Results were reported as specific activity (CPM/microgram protein). The results indicate that 10(-9) M TGF-beta 1 treated cultures showed a significant increase in RNA synthesis by HPDLF and HGF over time, as compared to the control cultures. HPDLF showed a significant increase in protein synthesis over time while that by HGF was not significant as compared to the control cultures. Lower concentrations of TGF-beta 1 demonstrated no significant differences from control. Results suggest that the effects of TGF-beta 1 on HPDLF and HGF are both time and dose dependent, with 10(-9) M TGF-beta 1 providing the best response of those concentrations tested. These findings support the concept that TGF-beta 1 may play a role in periodontal regeneration due to its ability to promote fibroblast RNA and protein synthesis. The results also demonstrate that although these two cells types appear morphologically similar, they exhibit distinct biological responses to growth factors such as TGF-beta 1.</description><identifier>ISSN: 0303-6979</identifier><identifier>DOI: 10.1111/j.1600-051X.1995.tb00826.x</identifier><identifier>PMID: 7593697</identifier><language>eng</language><publisher>United States</publisher><subject>Adolescent ; Adult ; Aged ; Cells, Cultured ; Colorimetry ; Culture Media ; Dentistry ; Dose-Response Relationship, Drug ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Gingiva - cytology ; Gingiva - drug effects ; Gingiva - metabolism ; Humans ; Methionine - metabolism ; Middle Aged ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Periodontal Ligament - metabolism ; Protein Biosynthesis ; Proteins - drug effects ; Regeneration ; RNA - biosynthesis ; RNA - drug effects ; Sulfur Radioisotopes ; Time Factors ; Transforming Growth Factor beta - administration & dosage ; Transforming Growth Factor beta - pharmacology ; Tritium ; Uridine - metabolism</subject><ispartof>Journal of clinical periodontology, 1995-09, Vol.22 (9), p.679-685</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7593697$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mailhot, J M</creatorcontrib><creatorcontrib>Schuster, G S</creatorcontrib><creatorcontrib>Garnick, J J</creatorcontrib><creatorcontrib>Hanes, P J</creatorcontrib><creatorcontrib>Lapp, C A</creatorcontrib><creatorcontrib>Lewis, J B</creatorcontrib><title>Human periodontal ligament and gingival fibroblast response to TGF-beta 1 stimulation</title><title>Journal of clinical periodontology</title><addtitle>J Clin Periodontol</addtitle><description>The purpose of this study was to measure the time-sequence response of RNA and protein synthesis to transforming growth factor-beta 1 (TGF-beta 1) by human periodontal ligament (HPDLF) and gingival (HGF) fibroblasts in culture. HPDLF and HGF were cultured from explants of healthy gingival tissue and freshly extracted teeth. Cultures of 8 x 10(4) cells/ml were exposed to medium containing 3H-uridine and 35S-methionine with TGF-beta 1 at concentrations from 10(-9) M to 10(-21) M, or control medium, for up to 60 hours in order to assess RNA and protein synthesis. Protein concentrations of comparable cultures were also assayed colorimetrically. Results were reported as specific activity (CPM/microgram protein). The results indicate that 10(-9) M TGF-beta 1 treated cultures showed a significant increase in RNA synthesis by HPDLF and HGF over time, as compared to the control cultures. HPDLF showed a significant increase in protein synthesis over time while that by HGF was not significant as compared to the control cultures. Lower concentrations of TGF-beta 1 demonstrated no significant differences from control. Results suggest that the effects of TGF-beta 1 on HPDLF and HGF are both time and dose dependent, with 10(-9) M TGF-beta 1 providing the best response of those concentrations tested. These findings support the concept that TGF-beta 1 may play a role in periodontal regeneration due to its ability to promote fibroblast RNA and protein synthesis. The results also demonstrate that although these two cells types appear morphologically similar, they exhibit distinct biological responses to growth factors such as TGF-beta 1.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Cells, Cultured</subject><subject>Colorimetry</subject><subject>Culture Media</subject><subject>Dentistry</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Gingiva - cytology</subject><subject>Gingiva - drug effects</subject><subject>Gingiva - metabolism</subject><subject>Humans</subject><subject>Methionine - metabolism</subject><subject>Middle Aged</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - drug effects</subject><subject>Periodontal Ligament - metabolism</subject><subject>Protein Biosynthesis</subject><subject>Proteins - drug effects</subject><subject>Regeneration</subject><subject>RNA - biosynthesis</subject><subject>RNA - drug effects</subject><subject>Sulfur Radioisotopes</subject><subject>Time Factors</subject><subject>Transforming Growth Factor beta - administration & dosage</subject><subject>Transforming Growth Factor beta - pharmacology</subject><subject>Tritium</subject><subject>Uridine - metabolism</subject><issn>0303-6979</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkE9LwzAchnNQ5px-BCF48NaaP23THGW4TRh42cBbSdpfRkaa1CYV_fYWtvfywsPDe3gReqYkp3NezzmtCMlISb9yKmWZJ01Izar89wYtCSc8q6SQd-g-xjMhVHDOF2ghSslnvkTH3dQrjwcYbeiCT8phZ0-qB5-w8h0-WX-yPzM1Vo9BOxUTHiEOwUfAKeDDdpNpSApTHJPtJ6eSDf4B3RrlIjxee4WOm_fDepftP7cf67d9NlBep4wzKaEWHSuVqJmuC8ElEcxU0AopdGEKww0roTSd4Uwr00GrKRCtOknbouIr9HLZHcbwPUFMTW9jC84pD2GKjRAVLeqCz-LTVZx0D10zjLZX419z_YH_AzLhYYQ</recordid><startdate>199509</startdate><enddate>199509</enddate><creator>Mailhot, J M</creator><creator>Schuster, G S</creator><creator>Garnick, J J</creator><creator>Hanes, P J</creator><creator>Lapp, C A</creator><creator>Lewis, J B</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199509</creationdate><title>Human periodontal ligament and gingival fibroblast response to TGF-beta 1 stimulation</title><author>Mailhot, J M ; Schuster, G S ; Garnick, J J ; Hanes, P J ; Lapp, C A ; Lewis, J B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p138t-3299e87d25a782b84739072f6ec797b4f4f3f25e5fdf32bafdecb1e0bad91c463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Cells, Cultured</topic><topic>Colorimetry</topic><topic>Culture Media</topic><topic>Dentistry</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Gingiva - cytology</topic><topic>Gingiva - drug effects</topic><topic>Gingiva - metabolism</topic><topic>Humans</topic><topic>Methionine - metabolism</topic><topic>Middle Aged</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - drug effects</topic><topic>Periodontal Ligament - metabolism</topic><topic>Protein Biosynthesis</topic><topic>Proteins - drug effects</topic><topic>Regeneration</topic><topic>RNA - biosynthesis</topic><topic>RNA - drug effects</topic><topic>Sulfur Radioisotopes</topic><topic>Time Factors</topic><topic>Transforming Growth Factor beta - administration & dosage</topic><topic>Transforming Growth Factor beta - pharmacology</topic><topic>Tritium</topic><topic>Uridine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mailhot, J M</creatorcontrib><creatorcontrib>Schuster, G S</creatorcontrib><creatorcontrib>Garnick, J J</creatorcontrib><creatorcontrib>Hanes, P J</creatorcontrib><creatorcontrib>Lapp, C A</creatorcontrib><creatorcontrib>Lewis, J B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical periodontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mailhot, J M</au><au>Schuster, G S</au><au>Garnick, J J</au><au>Hanes, P J</au><au>Lapp, C A</au><au>Lewis, J B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human periodontal ligament and gingival fibroblast response to TGF-beta 1 stimulation</atitle><jtitle>Journal of clinical periodontology</jtitle><addtitle>J Clin Periodontol</addtitle><date>1995-09</date><risdate>1995</risdate><volume>22</volume><issue>9</issue><spage>679</spage><epage>685</epage><pages>679-685</pages><issn>0303-6979</issn><abstract>The purpose of this study was to measure the time-sequence response of RNA and protein synthesis to transforming growth factor-beta 1 (TGF-beta 1) by human periodontal ligament (HPDLF) and gingival (HGF) fibroblasts in culture. HPDLF and HGF were cultured from explants of healthy gingival tissue and freshly extracted teeth. Cultures of 8 x 10(4) cells/ml were exposed to medium containing 3H-uridine and 35S-methionine with TGF-beta 1 at concentrations from 10(-9) M to 10(-21) M, or control medium, for up to 60 hours in order to assess RNA and protein synthesis. Protein concentrations of comparable cultures were also assayed colorimetrically. Results were reported as specific activity (CPM/microgram protein). The results indicate that 10(-9) M TGF-beta 1 treated cultures showed a significant increase in RNA synthesis by HPDLF and HGF over time, as compared to the control cultures. HPDLF showed a significant increase in protein synthesis over time while that by HGF was not significant as compared to the control cultures. Lower concentrations of TGF-beta 1 demonstrated no significant differences from control. Results suggest that the effects of TGF-beta 1 on HPDLF and HGF are both time and dose dependent, with 10(-9) M TGF-beta 1 providing the best response of those concentrations tested. These findings support the concept that TGF-beta 1 may play a role in periodontal regeneration due to its ability to promote fibroblast RNA and protein synthesis. The results also demonstrate that although these two cells types appear morphologically similar, they exhibit distinct biological responses to growth factors such as TGF-beta 1.</abstract><cop>United States</cop><pmid>7593697</pmid><doi>10.1111/j.1600-051X.1995.tb00826.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of clinical periodontology, 1995-09, Vol.22 (9), p.679-685 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adolescent Adult Aged Cells, Cultured Colorimetry Culture Media Dentistry Dose-Response Relationship, Drug Fibroblasts - drug effects Fibroblasts - metabolism Gingiva - cytology Gingiva - drug effects Gingiva - metabolism Humans Methionine - metabolism Middle Aged Periodontal Ligament - cytology Periodontal Ligament - drug effects Periodontal Ligament - metabolism Protein Biosynthesis Proteins - drug effects Regeneration RNA - biosynthesis RNA - drug effects Sulfur Radioisotopes Time Factors Transforming Growth Factor beta - administration & dosage Transforming Growth Factor beta - pharmacology Tritium Uridine - metabolism |
| title | Human periodontal ligament and gingival fibroblast response to TGF-beta 1 stimulation |
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