Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore

A low molecular weight protein (∼ 25,000 D) exhibiting a yellow fluorescence emission peaking at ∼ 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (λ max = 380, 460 nm) and th...

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Veröffentlicht in:	Biochemical and biophysical research communications 1987-07, Vol.146 (1), p.101-106
Hauptverfasser:	Macheroux, P., Schmidt, K.U., Steinerstauch, P., Ghisla, S., Colepicolo, P., Buntic, R., Hastings, J.W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences chromophores Exact sciences and technology flavin Flavin Mononucleotide Fluorescence Molecular Weight Other techniques and industries Spectrometry, Fluorescence Vibrio - analysis Vibrio fischeri Viral Proteins - isolation & purification
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