Rapid procedure for preparation of macrophage plasma membrane
1. 1. This report describes a simple and efficient procedure for the isolation of plasma membrane from guinea pig peritoneal macrophages. The use of polycationic beads (Affi-gel 731 beads) facilities rapid and high-clear separation of plasma membrane within 30 min. 2. 2. The final plasma membrane co...
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| Veröffentlicht in: | International journal of biochemistry 1987, Vol.19 (6), p.489-493 |
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| container_end_page | 493 |
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| container_issue | 6 |
| container_start_page | 489 |
| container_title | International journal of biochemistry |
| container_volume | 19 |
| creator | Shibata, Yasuko Abiko, Yoshimitsu Arii, Hiroshi Sone, Masayuki Takiguchi, Hisashi |
| description | 1.
1. This report describes a simple and efficient procedure for the isolation of plasma membrane from guinea pig peritoneal macrophages. The use of polycationic beads (Affi-gel 731 beads) facilities rapid and high-clear separation of plasma membrane within 30 min.
2.
2. The final plasma membrane coated beads fraction has high specific activities of marker enzymes with little contamination with mitochondrial, lysosomal or cytoplasmal markers. |
| doi_str_mv | 10.1016/0020-711X(87)90131-5 |
| format | Article |
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1. This report describes a simple and efficient procedure for the isolation of plasma membrane from guinea pig peritoneal macrophages. The use of polycationic beads (Affi-gel 731 beads) facilities rapid and high-clear separation of plasma membrane within 30 min.
2.
2. The final plasma membrane coated beads fraction has high specific activities of marker enzymes with little contamination with mitochondrial, lysosomal or cytoplasmal markers.</description><identifier>ISSN: 0020-711X</identifier><identifier>DOI: 10.1016/0020-711X(87)90131-5</identifier><identifier>PMID: 3038633</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Cell Fractionation - methods ; Cell Membrane - enzymology ; Cell Membrane - ultrastructure ; Guinea Pigs ; macrophages ; Macrophages - ultrastructure ; Male ; Microscopy, Electron, Scanning ; Peritoneal Cavity - cytology ; Phospholipases A - metabolism ; plasma membranes ; Sodium-Potassium-Exchanging ATPase - metabolism</subject><ispartof>International journal of biochemistry, 1987, Vol.19 (6), p.489-493</ispartof><rights>1987</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-c7eabb1dcd88cb624913e3e2c11d9c6b0b17dea8e40799962faee63ca0ad41263</citedby><cites>FETCH-LOGICAL-c388t-c7eabb1dcd88cb624913e3e2c11d9c6b0b17dea8e40799962faee63ca0ad41263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3038633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shibata, Yasuko</creatorcontrib><creatorcontrib>Abiko, Yoshimitsu</creatorcontrib><creatorcontrib>Arii, Hiroshi</creatorcontrib><creatorcontrib>Sone, Masayuki</creatorcontrib><creatorcontrib>Takiguchi, Hisashi</creatorcontrib><title>Rapid procedure for preparation of macrophage plasma membrane</title><title>International journal of biochemistry</title><addtitle>Int J Biochem</addtitle><description>1.
1. This report describes a simple and efficient procedure for the isolation of plasma membrane from guinea pig peritoneal macrophages. The use of polycationic beads (Affi-gel 731 beads) facilities rapid and high-clear separation of plasma membrane within 30 min.
2.
2. The final plasma membrane coated beads fraction has high specific activities of marker enzymes with little contamination with mitochondrial, lysosomal or cytoplasmal markers.</description><subject>Animals</subject><subject>Cell Fractionation - methods</subject><subject>Cell Membrane - enzymology</subject><subject>Cell Membrane - ultrastructure</subject><subject>Guinea Pigs</subject><subject>macrophages</subject><subject>Macrophages - ultrastructure</subject><subject>Male</subject><subject>Microscopy, Electron, Scanning</subject><subject>Peritoneal Cavity - cytology</subject><subject>Phospholipases A - metabolism</subject><subject>plasma membranes</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><issn>0020-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhnNQ1nX1Hyj0JHqoZppskx4UZPELFgRR8BbSZKqRdluTVvDfm7rLHvU0zLzvfD2EHAE9Bwr5BaUZTQXA66kUZwUFBul8h0y35T2yH8IHpVBIDhMyYZTJnLEpuXzSnbNJ51uDdvCYVK2PGXba6961q6StkkYb33bv-g2Trtah0UmDTen1Cg_IbqXrgIebOCMvtzfPi_t0-Xj3sLhepoZJ2adGoC5LsMZKaco84wUwZJgZAFuYvKQlCItaIqeiKIo8qzRizoym2nLIcjYjJ-u58c7PAUOvGhcM1nW8oR2CEiIHTvn_RuBC0mw-GvnaGF8LwWOlOu8a7b8VUDUiVSM7NbJTUqhfpGoe244384eyQbtt2vCM-tVax0jjy6FXwThcRbbOo-mVbd3fC34AmOSH6Q</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Shibata, Yasuko</creator><creator>Abiko, Yoshimitsu</creator><creator>Arii, Hiroshi</creator><creator>Sone, Masayuki</creator><creator>Takiguchi, Hisashi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Rapid procedure for preparation of macrophage plasma membrane</title><author>Shibata, Yasuko ; Abiko, Yoshimitsu ; Arii, Hiroshi ; Sone, Masayuki ; Takiguchi, Hisashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-c7eabb1dcd88cb624913e3e2c11d9c6b0b17dea8e40799962faee63ca0ad41263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Cell Fractionation - methods</topic><topic>Cell Membrane - enzymology</topic><topic>Cell Membrane - ultrastructure</topic><topic>Guinea Pigs</topic><topic>macrophages</topic><topic>Macrophages - ultrastructure</topic><topic>Male</topic><topic>Microscopy, Electron, Scanning</topic><topic>Peritoneal Cavity - cytology</topic><topic>Phospholipases A - metabolism</topic><topic>plasma membranes</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Shibata, Yasuko</creatorcontrib><creatorcontrib>Abiko, Yoshimitsu</creatorcontrib><creatorcontrib>Arii, Hiroshi</creatorcontrib><creatorcontrib>Sone, Masayuki</creatorcontrib><creatorcontrib>Takiguchi, Hisashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shibata, Yasuko</au><au>Abiko, Yoshimitsu</au><au>Arii, Hiroshi</au><au>Sone, Masayuki</au><au>Takiguchi, Hisashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid procedure for preparation of macrophage plasma membrane</atitle><jtitle>International journal of biochemistry</jtitle><addtitle>Int J Biochem</addtitle><date>1987</date><risdate>1987</risdate><volume>19</volume><issue>6</issue><spage>489</spage><epage>493</epage><pages>489-493</pages><issn>0020-711X</issn><abstract>1.
1. This report describes a simple and efficient procedure for the isolation of plasma membrane from guinea pig peritoneal macrophages. The use of polycationic beads (Affi-gel 731 beads) facilities rapid and high-clear separation of plasma membrane within 30 min.
2.
2. The final plasma membrane coated beads fraction has high specific activities of marker enzymes with little contamination with mitochondrial, lysosomal or cytoplasmal markers.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>3038633</pmid><doi>10.1016/0020-711X(87)90131-5</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-711X |
| ispartof | International journal of biochemistry, 1987, Vol.19 (6), p.489-493 |
| issn | 0020-711X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77614046 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Fractionation - methods Cell Membrane - enzymology Cell Membrane - ultrastructure Guinea Pigs macrophages Macrophages - ultrastructure Male Microscopy, Electron, Scanning Peritoneal Cavity - cytology Phospholipases A - metabolism plasma membranes Sodium-Potassium-Exchanging ATPase - metabolism |
| title | Rapid procedure for preparation of macrophage plasma membrane |
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