Epidermal Surface Antigen (M517S1) Is Highly Conserved between Mouse and Human
A mouse monoclonal antibody ECS-1 raised to human keratinocytes detects a 35-kDa epidermal surface antigen (ESA) and causes keratinocyte dissociation in vitro. ECS-1 stains skin of 16-day mouse embryo and 8- to 9-week human fetus. Mouse Esa cDNA encodes a 379-amino-acid protein that is 99.2% identic...
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| Veröffentlicht in: | Genomics 1995-05, Vol.27 (2), p.251-258 |
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| creator | Cho, Yoon-Jae Chema, Deidra Moskow, John J. Cho, Mimi Schroeder, Wanda T. Overbeek, Paul Buchberg, Arthur M. Duvic, Madeleine |
| description | A mouse monoclonal antibody ECS-1 raised to human keratinocytes detects a 35-kDa epidermal surface antigen (ESA) and causes keratinocyte dissociation in vitro. ECS-1 stains skin of 16-day mouse embryo and 8- to 9-week human fetus. Mouse Esa cDNA encodes a 379-amino-acid protein that is 99.2% identical to the human, differing at only 3 amino acids. The gene (M17S1) was mapped to mouse chromosome 11, highlighting the conserved linkage synteny existing between human chromosome 17 and mouse chromosome 11. Although the nude locus has been mapped to the same region of chromosome 11, no abnormalities in protein, mRNA, or cDNA or genomic sequences were detected in nude mice. However, both nude and control mice were found to have a second Esa mRNA transcript that conserves amino acid sequence and molecular weight. The mouse and human 5′ and 3′ untranslated sequences are conserved. Similar RNA folding patterns of the 5′ untranslated region are predicted despite a 91-bp insertion in the mouse. These data suggest that both the function and the regulation of ESA protein are of importance and that Esa (M17S1) is not the nude locus gene. |
| doi_str_mv | 10.1006/geno.1995.1039 |
| format | Article |
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ECS-1 stains skin of 16-day mouse embryo and 8- to 9-week human fetus. Mouse Esa cDNA encodes a 379-amino-acid protein that is 99.2% identical to the human, differing at only 3 amino acids. The gene (M17S1) was mapped to mouse chromosome 11, highlighting the conserved linkage synteny existing between human chromosome 17 and mouse chromosome 11. Although the nude locus has been mapped to the same region of chromosome 11, no abnormalities in protein, mRNA, or cDNA or genomic sequences were detected in nude mice. However, both nude and control mice were found to have a second Esa mRNA transcript that conserves amino acid sequence and molecular weight. The mouse and human 5′ and 3′ untranslated sequences are conserved. Similar RNA folding patterns of the 5′ untranslated region are predicted despite a 91-bp insertion in the mouse. These data suggest that both the function and the regulation of ESA protein are of importance and that Esa (M17S1) is not the nude locus gene.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1995.1039</identifier><identifier>PMID: 7557989</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; ANTIGENS ; Antigens, Surface - analysis ; Antigens, Surface - genetics ; Base Sequence ; Biological and medical sciences ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Chromosome Mapping ; Chromosomes - genetics ; Conserved Sequence ; DNA SEQUENCING ; DNA, Complementary - chemistry ; EPIDERMIS ; Fundamental and applied biological sciences. Psychology ; GENES ; Genes. Genome ; GENETIC MAPPING ; HUMAN CHROMOSOME 17 ; Humans ; Membrane Proteins ; Mice ; Mice, Inbred C57BL ; Mice, Inbred Strains ; Mice, Nude ; Models, Molecular ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; RNA, Messenger - chemistry ; Skin - chemistry</subject><ispartof>Genomics, 1995-05, Vol.27 (2), p.251-258</ispartof><rights>1995 Academic Press</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-c426a6ca3c12501e4d972e178eb0ea9cba5ea71f668920d57457c77806a96c6a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/geno.1995.1039$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3581561$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7557989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/114833$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Yoon-Jae</creatorcontrib><creatorcontrib>Chema, Deidra</creatorcontrib><creatorcontrib>Moskow, John J.</creatorcontrib><creatorcontrib>Cho, Mimi</creatorcontrib><creatorcontrib>Schroeder, Wanda T.</creatorcontrib><creatorcontrib>Overbeek, Paul</creatorcontrib><creatorcontrib>Buchberg, Arthur M.</creatorcontrib><creatorcontrib>Duvic, Madeleine</creatorcontrib><title>Epidermal Surface Antigen (M517S1) Is Highly Conserved between Mouse and Human</title><title>Genomics</title><addtitle>Genomics</addtitle><description>A mouse monoclonal antibody ECS-1 raised to human keratinocytes detects a 35-kDa epidermal surface antigen (ESA) and causes keratinocyte dissociation in vitro. ECS-1 stains skin of 16-day mouse embryo and 8- to 9-week human fetus. Mouse Esa cDNA encodes a 379-amino-acid protein that is 99.2% identical to the human, differing at only 3 amino acids. The gene (M17S1) was mapped to mouse chromosome 11, highlighting the conserved linkage synteny existing between human chromosome 17 and mouse chromosome 11. Although the nude locus has been mapped to the same region of chromosome 11, no abnormalities in protein, mRNA, or cDNA or genomic sequences were detected in nude mice. However, both nude and control mice were found to have a second Esa mRNA transcript that conserves amino acid sequence and molecular weight. The mouse and human 5′ and 3′ untranslated sequences are conserved. Similar RNA folding patterns of the 5′ untranslated region are predicted despite a 91-bp insertion in the mouse. These data suggest that both the function and the regulation of ESA protein are of importance and that Esa (M17S1) is not the nude locus gene.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>ANTIGENS</subject><subject>Antigens, Surface - analysis</subject><subject>Antigens, Surface - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Chromosome Mapping</subject><subject>Chromosomes - genetics</subject><subject>Conserved Sequence</subject><subject>DNA SEQUENCING</subject><subject>DNA, Complementary - chemistry</subject><subject>EPIDERMIS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENES</subject><subject>Genes. Genome</subject><subject>GENETIC MAPPING</subject><subject>HUMAN CHROMOSOME 17</subject><subject>Humans</subject><subject>Membrane Proteins</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred Strains</subject><subject>Mice, Nude</subject><subject>Models, Molecular</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>RNA, Messenger - chemistry</subject><subject>Skin - chemistry</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpSLdpr70VVAilOexWY1lfx7Ak2UCSHtKehVYeJyq2vJXslPz7yuySW8lFg5hn3vl4CfkEbAWMye8PGIcVGCPKl5s3ZAFMm6WWtXxLFkxrvVSi5u_I-5x_M8YM19UxOVZCKKPNgtxd7EKDqXcdvZ9S6zzS8ziGokq_3QpQ93BGrzPdhIfH7pmuh5gxPWFDtzj-xQLdDlNG6mJDN1Pv4gdy1Lou48dDPCG_Li9-rjfLmx9X1-vzm6WvKzHOr3TSO-6hEgywboyqEJTGLUNn_NYJdApaKbWpWCNULZRXSjPpjPTS8RPyZa875DHY7MOI_tEPMaIfLUCtOS_M1z2zS8OfCfNo-5A9dp2LWKa2SkkAoV4HQUjGgFcFXO1Bn4acE7Z2l0Lv0rMFZmc37OyGnd2wsxul4PNBedr22Lzgh_OX_Okh77J3XZtc9CG_YFzo0hsKpvcYlpM-BUzzxhg9NiHNCzdD-N8E_wDPY6K0</recordid><startdate>19950520</startdate><enddate>19950520</enddate><creator>Cho, Yoon-Jae</creator><creator>Chema, Deidra</creator><creator>Moskow, John J.</creator><creator>Cho, Mimi</creator><creator>Schroeder, Wanda T.</creator><creator>Overbeek, Paul</creator><creator>Buchberg, Arthur M.</creator><creator>Duvic, Madeleine</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19950520</creationdate><title>Epidermal Surface Antigen (M517S1) Is Highly Conserved between Mouse and Human</title><author>Cho, Yoon-Jae ; Chema, Deidra ; Moskow, John J. ; Cho, Mimi ; Schroeder, Wanda T. ; Overbeek, Paul ; Buchberg, Arthur M. ; Duvic, Madeleine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-c426a6ca3c12501e4d972e178eb0ea9cba5ea71f668920d57457c77806a96c6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>ANTIGENS</topic><topic>Antigens, Surface - analysis</topic><topic>Antigens, Surface - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Chromosome Mapping</topic><topic>Chromosomes - genetics</topic><topic>Conserved Sequence</topic><topic>DNA SEQUENCING</topic><topic>DNA, Complementary - chemistry</topic><topic>EPIDERMIS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENES</topic><topic>Genes. Genome</topic><topic>GENETIC MAPPING</topic><topic>HUMAN CHROMOSOME 17</topic><topic>Humans</topic><topic>Membrane Proteins</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred Strains</topic><topic>Mice, Nude</topic><topic>Models, Molecular</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>RNA, Messenger - chemistry</topic><topic>Skin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cho, Yoon-Jae</creatorcontrib><creatorcontrib>Chema, Deidra</creatorcontrib><creatorcontrib>Moskow, John J.</creatorcontrib><creatorcontrib>Cho, Mimi</creatorcontrib><creatorcontrib>Schroeder, Wanda T.</creatorcontrib><creatorcontrib>Overbeek, Paul</creatorcontrib><creatorcontrib>Buchberg, Arthur M.</creatorcontrib><creatorcontrib>Duvic, Madeleine</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, Yoon-Jae</au><au>Chema, Deidra</au><au>Moskow, John J.</au><au>Cho, Mimi</au><au>Schroeder, Wanda T.</au><au>Overbeek, Paul</au><au>Buchberg, Arthur M.</au><au>Duvic, Madeleine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epidermal Surface Antigen (M517S1) Is Highly Conserved between Mouse and Human</atitle><jtitle>Genomics</jtitle><addtitle>Genomics</addtitle><date>1995-05-20</date><risdate>1995</risdate><volume>27</volume><issue>2</issue><spage>251</spage><epage>258</epage><pages>251-258</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>A mouse monoclonal antibody ECS-1 raised to human keratinocytes detects a 35-kDa epidermal surface antigen (ESA) and causes keratinocyte dissociation in vitro. ECS-1 stains skin of 16-day mouse embryo and 8- to 9-week human fetus. Mouse Esa cDNA encodes a 379-amino-acid protein that is 99.2% identical to the human, differing at only 3 amino acids. The gene (M17S1) was mapped to mouse chromosome 11, highlighting the conserved linkage synteny existing between human chromosome 17 and mouse chromosome 11. Although the nude locus has been mapped to the same region of chromosome 11, no abnormalities in protein, mRNA, or cDNA or genomic sequences were detected in nude mice. However, both nude and control mice were found to have a second Esa mRNA transcript that conserves amino acid sequence and molecular weight. The mouse and human 5′ and 3′ untranslated sequences are conserved. Similar RNA folding patterns of the 5′ untranslated region are predicted despite a 91-bp insertion in the mouse. These data suggest that both the function and the regulation of ESA protein are of importance and that Esa (M17S1) is not the nude locus gene.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7557989</pmid><doi>10.1006/geno.1995.1039</doi><tpages>8</tpages></addata></record> |
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| ispartof | Genomics, 1995-05, Vol.27 (2), p.251-258 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Animals ANTIGENS Antigens, Surface - analysis Antigens, Surface - genetics Base Sequence Biological and medical sciences BIOLOGY AND MEDICINE, BASIC STUDIES Chromosome Mapping Chromosomes - genetics Conserved Sequence DNA SEQUENCING DNA, Complementary - chemistry EPIDERMIS Fundamental and applied biological sciences. Psychology GENES Genes. Genome GENETIC MAPPING HUMAN CHROMOSOME 17 Humans Membrane Proteins Mice Mice, Inbred C57BL Mice, Inbred Strains Mice, Nude Models, Molecular Molecular and cellular biology Molecular genetics Molecular Sequence Data RNA, Messenger - chemistry Skin - chemistry |
| title | Epidermal Surface Antigen (M517S1) Is Highly Conserved between Mouse and Human |
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