Cloning and Heterologous Expression of a Gene Cluster for the Biosynthesis of Tetracenomycin C, the Anthracycline Antitumor Antibiotic of Streptomyces glaucescens
Through complementation of mutations specifically blocking the biosynthesis of tetracenomycin C by Streptomyces glaucescens and selecting for resistance to tetracenomycin C in Streptomyces lividans, all of the genes for the production of tetracenomycin C were inserted in pIJ702, a high copy-number S...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1987-07, Vol.84 (13), p.4445-4449 |
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| container_issue | 13 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 84 |
| creator | Motamedi, Haideh Hutchinson, C. Richard |
| description | Through complementation of mutations specifically blocking the biosynthesis of tetracenomycin C by Streptomyces glaucescens and selecting for resistance to tetracenomycin C in Streptomyces lividans, all of the genes for the production of tetracenomycin C were inserted in pIJ702, a high copy-number Streptomyces gene cloning vector. The tcm biosynthetic and resistance genes occur as a single cluster in the S. glaucescens genome and are expressed in heterologous streptomycete hosts like S. lividans, resulting in the overproduction of pigmented intermediates of the tetracenomycin C biosynthetic pathway. |
| doi_str_mv | 10.1073/pnas.84.13.4445 |
| format | Article |
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The tcm biosynthetic and resistance genes occur as a single cluster in the S. glaucescens genome and are expressed in heterologous streptomycete hosts like S. lividans, resulting in the overproduction of pigmented intermediates of the tetracenomycin C biosynthetic pathway.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.13.4445</identifier><identifier>PMID: 3474613</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Anthracyclines ; Antibiotics ; Bacteriology ; Biological and medical sciences ; Biosynthesis ; cloning ; Cloning, Molecular ; Complementation ; DNA probes ; Drug Resistance, Microbial ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; gene expression ; genes ; Genes, Fungal ; Genetic Complementation Test ; Genetic mutation ; Genetic vectors ; Genetics ; Metabolites ; Microbiology ; Naphthacenes - biosynthesis ; Naphthacenes - pharmacology ; Plasmids ; Streptomyces ; Streptomyces - drug effects ; Streptomyces - genetics ; Streptomyces - metabolism ; Streptomyces glaucescens ; tetracenomycin C</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1987-07, Vol.84 (13), p.4445-4449</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c587t-3f4faa18b6ea4ca7cc1a60334bb51e88dc4cf61d03993833c41c5e920cd729303</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/84/13.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30191$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30191$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8323430$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3474613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Motamedi, Haideh</creatorcontrib><creatorcontrib>Hutchinson, C. Richard</creatorcontrib><title>Cloning and Heterologous Expression of a Gene Cluster for the Biosynthesis of Tetracenomycin C, the Anthracycline Antitumor Antibiotic of Streptomyces glaucescens</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Through complementation of mutations specifically blocking the biosynthesis of tetracenomycin C by Streptomyces glaucescens and selecting for resistance to tetracenomycin C in Streptomyces lividans, all of the genes for the production of tetracenomycin C were inserted in pIJ702, a high copy-number Streptomyces gene cloning vector. The tcm biosynthetic and resistance genes occur as a single cluster in the S. glaucescens genome and are expressed in heterologous streptomycete hosts like S. lividans, resulting in the overproduction of pigmented intermediates of the tetracenomycin C biosynthetic pathway.</description><subject>Anthracyclines</subject><subject>Antibiotics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>Complementation</subject><subject>DNA probes</subject><subject>Drug Resistance, Microbial</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>gene expression</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>Genetic Complementation Test</subject><subject>Genetic mutation</subject><subject>Genetic vectors</subject><subject>Genetics</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>Naphthacenes - biosynthesis</subject><subject>Naphthacenes - pharmacology</subject><subject>Plasmids</subject><subject>Streptomyces</subject><subject>Streptomyces - drug effects</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces glaucescens</subject><subject>tetracenomycin C</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKkvhjIQE8gHBhWzt2EmcA4cSlRapEgfK2XIcZ-vKsVPbQd2_wy_F2Y0CXOA0Y8_zzicALzHaYlSRs9GKsGV0i8mWUlo8AhuMapyVtEaPwQahvMoYzelT8CyEO4RQXTB0Ak4IrWiJyQb8bIyz2u6gsB28UlF5Z9zOTQFePIxehaCdha6HAl4qq2BjppAY2DsP462Cn7QLe5u8oMOM3ajohVTWDXupLWw-HKjzRKTvvTTaHl46TkPKMHutdlHLWfstejXGWakC3BkxJZtShefgSS9MUC8Wewq-f764aa6y66-XX5rz60wWrIoZ6WkvBGZtqQSVopISixIRQtu2wIqxTlLZl7hDpK4JI0RSLAtV50h2VV4TRE7Bx2PecWoH1aXSaRbDR68H4ffcCc3_jlh9y3fuByeowKhM-neL3rv7SYXIB50GMEZYlRbKq6rEiKbi_wMxrQqKizyBZ0dQeheCV_3aDEZ8Pj-fz88Z5Zjw-fxJ8frPGVZ-uXeKv13iIkhhei-s1GHFGMkJPazi_YLN-dfoWof3kzFRPcREvvknmYBXR-AuROd_N4Rwjckv6gzeLA</recordid><startdate>19870701</startdate><enddate>19870701</enddate><creator>Motamedi, Haideh</creator><creator>Hutchinson, C. Richard</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870701</creationdate><title>Cloning and Heterologous Expression of a Gene Cluster for the Biosynthesis of Tetracenomycin C, the Anthracycline Antitumor Antibiotic of Streptomyces glaucescens</title><author>Motamedi, Haideh ; Hutchinson, C. Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c587t-3f4faa18b6ea4ca7cc1a60334bb51e88dc4cf61d03993833c41c5e920cd729303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Anthracyclines</topic><topic>Antibiotics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>Complementation</topic><topic>DNA probes</topic><topic>Drug Resistance, Microbial</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>gene expression</topic><topic>genes</topic><topic>Genes, Fungal</topic><topic>Genetic Complementation Test</topic><topic>Genetic mutation</topic><topic>Genetic vectors</topic><topic>Genetics</topic><topic>Metabolites</topic><topic>Microbiology</topic><topic>Naphthacenes - biosynthesis</topic><topic>Naphthacenes - pharmacology</topic><topic>Plasmids</topic><topic>Streptomyces</topic><topic>Streptomyces - drug effects</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><topic>Streptomyces glaucescens</topic><topic>tetracenomycin C</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Motamedi, Haideh</creatorcontrib><creatorcontrib>Hutchinson, C. Richard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Motamedi, Haideh</au><au>Hutchinson, C. Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Heterologous Expression of a Gene Cluster for the Biosynthesis of Tetracenomycin C, the Anthracycline Antitumor Antibiotic of Streptomyces glaucescens</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-07-01</date><risdate>1987</risdate><volume>84</volume><issue>13</issue><spage>4445</spage><epage>4449</epage><pages>4445-4449</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Through complementation of mutations specifically blocking the biosynthesis of tetracenomycin C by Streptomyces glaucescens and selecting for resistance to tetracenomycin C in Streptomyces lividans, all of the genes for the production of tetracenomycin C were inserted in pIJ702, a high copy-number Streptomyces gene cloning vector. The tcm biosynthetic and resistance genes occur as a single cluster in the S. glaucescens genome and are expressed in heterologous streptomycete hosts like S. lividans, resulting in the overproduction of pigmented intermediates of the tetracenomycin C biosynthetic pathway.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3474613</pmid><doi>10.1073/pnas.84.13.4445</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1987-07, Vol.84 (13), p.4445-4449 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Anthracyclines Antibiotics Bacteriology Biological and medical sciences Biosynthesis cloning Cloning, Molecular Complementation DNA probes Drug Resistance, Microbial Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism gene expression genes Genes, Fungal Genetic Complementation Test Genetic mutation Genetic vectors Genetics Metabolites Microbiology Naphthacenes - biosynthesis Naphthacenes - pharmacology Plasmids Streptomyces Streptomyces - drug effects Streptomyces - genetics Streptomyces - metabolism Streptomyces glaucescens tetracenomycin C |
| title | Cloning and Heterologous Expression of a Gene Cluster for the Biosynthesis of Tetracenomycin C, the Anthracycline Antitumor Antibiotic of Streptomyces glaucescens |
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