Analysis of the mechanism of action of bradykinin on human basilar artery in vitro

Bradykinin (BK) initially produced concentration-related relaxations of human basilar artery in vitro. Concentration-effect curves constructed at 2 h intervals to BK over an 8 h period were reproducible. The rank order of potency of three kinins on the human basilar artery was found to be BK greater...

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Veröffentlicht in:Naunyn-Schmiedeberg's archives of pharmacology 1987-04, Vol.335 (4), p.433-437
Hauptverfasser: WHALLEY, E. T, AMURE, Y. O, LYE, R. H
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description Bradykinin (BK) initially produced concentration-related relaxations of human basilar artery in vitro. Concentration-effect curves constructed at 2 h intervals to BK over an 8 h period were reproducible. The rank order of potency of three kinins on the human basilar artery was found to be BK greater than methionyl-lysyl-BK greater than des-Arg9-BK. The B2-receptor antagonist Thi5,8, D-Phe7-BK but not the B1-receptor antagonist des-Arg9-Leu8-BK selectively blocked BK-induced relaxations of the human basilar artery. The relaxant effects of bradykinin and acetylcholine but not papaverine were attenuated after removal of the endothelium or treating the tissues with BW755C. Indomethacin was without effect. Concentration-effect curves to angiotensin I were markedly attenuated by captopril at a concentration which had no effect on BK, angiotensin II or 5-hydroxytryptamine responses. It is concluded that BK induced relaxations of the human basilar artery are mediated via activation of a B2 receptor and the response is dependent upon the release of a factor present in the endothelium. Angiotensin converting enzyme is present in the human basilar artery and is important for the conversion of angiotensin I to angiotensin II but apparently not for the degradation of BK. It is likely that other kininases are present and active in the tissue.
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Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Muscle Relaxation - drug effects</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Papaverine - pharmacology</subject><subject>Peptidyl-Dipeptidase A - metabolism</subject><subject>Vertebrates: cardiovascular system</subject><issn>0028-1298</issn><issn>1432-1912</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtLAzEQxoMotT4u3oU9iAdhdSZpNs2xilVBEETPy2ya0Og-arIV9r83pdWrzGEe34-P4WPsDOEaAdRN5QCwkFLqPTbGieA5auT7bAzApzlyPT1kRzF-AECBUo7YSIBQQuOYvc5aqofoY9a5rF_arLFmSa2PzeZApvddu5mqQIvh07c-bW22XDfUZhVFX1PIKPQ2DFmSvn0fuhN24KiO9nTXj9n7_P7t7jF_fnl4ups950Zw0efWgXFCcmW1gcouFDoEnAA6zTUprW2xqBCISzklmT7n2gldoEokyaISx-xy67sK3dfaxr5sfDS2rqm13TqWShWQqvgXxInCIn2SwKstaEIXY7CuXAXfUBhKhHKTdHk7_006wec713XV2MUfuos26Rc7naKh2gVqjY9_mJIClQLxA8UUg8U</recordid><startdate>198704</startdate><enddate>198704</enddate><creator>WHALLEY, E. 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Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Muscle Relaxation - drug effects</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Papaverine - pharmacology</topic><topic>Peptidyl-Dipeptidase A - metabolism</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WHALLEY, E. T</creatorcontrib><creatorcontrib>AMURE, Y. O</creatorcontrib><creatorcontrib>LYE, R. 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H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the mechanism of action of bradykinin on human basilar artery in vitro</atitle><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><date>1987-04</date><risdate>1987</risdate><volume>335</volume><issue>4</issue><spage>433</spage><epage>437</epage><pages>433-437</pages><issn>0028-1298</issn><eissn>1432-1912</eissn><coden>NSAPCC</coden><abstract>Bradykinin (BK) initially produced concentration-related relaxations of human basilar artery in vitro. Concentration-effect curves constructed at 2 h intervals to BK over an 8 h period were reproducible. The rank order of potency of three kinins on the human basilar artery was found to be BK greater than methionyl-lysyl-BK greater than des-Arg9-BK. 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ispartof Naunyn-Schmiedeberg's archives of pharmacology, 1987-04, Vol.335 (4), p.433-437
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language eng
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source MEDLINE; SpringerNature Journals
subjects Acetylcholine - metabolism
basilar artery
Basilar Artery - drug effects
Biological and medical sciences
Blood vessels and receptors
bradykinin
Bradykinin - pharmacology
Endothelium - physiology
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Muscle Relaxation - drug effects
Muscle, Smooth, Vascular - drug effects
Papaverine - pharmacology
Peptidyl-Dipeptidase A - metabolism
Vertebrates: cardiovascular system
title Analysis of the mechanism of action of bradykinin on human basilar artery in vitro
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