Rapid cloning of multiple amplified nucleotide sequences from human neuroblastoma cell lines by phenol emulsion competitive DNA reassociation

A protocol for the rapid cloning of many DNA fragments from an amplified genomic region is described. The procedure is based on a modification of the phenol-emulsion reassociation technique (PERT) previously used to clone DNA fragments missing from a chromosomal deletion [Kunkel et al., Proc. Natl....

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Veröffentlicht in:	Gene 1987, Vol.51 (1), p.53-59
Hauptverfasser:	Shiloh, Yosef, Rose, Elise, Colletti-Feener, Chris, Korf, Bruce, Kunkel, Louis M., Latt, Samuel A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Biotechnology Cell Line cloning Cloning, Molecular - methods DNA DNA, Neoplasm - genetics DNA, Neoplasm - isolation & purification DNA, Recombinant - isolation & purification Emulsions Fundamental and applied biological sciences. Psychology gene libraries Genetic engineering Genetic technics Humans man Methods. Procedures. Technologies Molecular cloning Neuroblastoma - genetics neuroblastoma cells neuroectoderm tumors Nucleic Acid Hybridization PERT methodology Phenol Phenols Recombinant DNA
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