The survival of adult mouse sensory neurons in vitro is enhanced by natural and synthetic substrata, particularly fibronectin
Primary cultures derived from adult mouse dorsal root ganglia have been maintained in the presence or absence of 5 × 10 −6M cytosine arabinoside for periods of up to 4 weeks. In cultures in which cytosine arabinoside is present, the non‐neuronal cell population is effectively reduced. When uncoated...
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| Veröffentlicht in: | Journal of neuroscience research 1987, Vol.17 (3), p.265-270 |
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| creator | Smith, R. A. Orr, D. J. |
| description | Primary cultures derived from adult mouse dorsal root ganglia have been maintained in the presence or absence of 5 × 10 −6M cytosine arabinoside for periods of up to 4 weeks. In cultures in which cytosine arabinoside is present, the non‐neuronal cell population is effectively reduced. When uncoated plastic substrata are used there is also a concurrent decrease in the number of neurons if the medium is supplemented with cytosine arabinoside. The effects on neuron survival of substrata coated with fibronectin, polyornithine, polylysine, and exudates prepared from mouse liver cells were studied. It was shown that neuronal densities similar to those with uninhibited media may be retained in the presence of cytosine arabinoside if fibronectin‐coated substrata are prepared. With the other coating agents neuronal survival was also enhanced but to a lesser extent. The study offers a means therefore of producing purer cultures of dorsal root ganglia neurons than has previously been possible from adult mammalian sources. |
| doi_str_mv | 10.1002/jnr.490170310 |
| format | Article |
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A. ; Orr, D. J.</creator><creatorcontrib>Smith, R. A. ; Orr, D. J.</creatorcontrib><description>Primary cultures derived from adult mouse dorsal root ganglia have been maintained in the presence or absence of 5 × 10 −6M cytosine arabinoside for periods of up to 4 weeks. In cultures in which cytosine arabinoside is present, the non‐neuronal cell population is effectively reduced. When uncoated plastic substrata are used there is also a concurrent decrease in the number of neurons if the medium is supplemented with cytosine arabinoside. The effects on neuron survival of substrata coated with fibronectin, polyornithine, polylysine, and exudates prepared from mouse liver cells were studied. It was shown that neuronal densities similar to those with uninhibited media may be retained in the presence of cytosine arabinoside if fibronectin‐coated substrata are prepared. With the other coating agents neuronal survival was also enhanced but to a lesser extent. The study offers a means therefore of producing purer cultures of dorsal root ganglia neurons than has previously been possible from adult mammalian sources.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.490170310</identifier><identifier>PMID: 3298663</identifier><identifier>CODEN: JNREDK</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Cell Count ; Cell Survival ; Cells, Cultured ; cultured adult neurons ; Cytarabine - pharmacology ; DRG ; fibronectin ; Fibronectins ; Fundamental and applied biological sciences. Psychology ; Ganglia, Spinal ; Isolated neuron and nerve. Neuroglia ; liver exudates ; Mice ; Mice, Inbred CBA ; Microscopy, Phase-Contrast ; Neurons, Afferent ; Peptides ; Polylysine ; polyornithine ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience research, 1987, Vol.17 (3), p.265-270</ispartof><rights>Copyright © 1987 Alan R. Liss, Inc.</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3490-a76539b8a7ead287bbfc363a3758a9568354a678e383f2263efd24c376daac833</citedby><cites>FETCH-LOGICAL-c3490-a76539b8a7ead287bbfc363a3758a9568354a678e383f2263efd24c376daac833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.490170310$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.490170310$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8227391$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3298663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, R. A.</creatorcontrib><creatorcontrib>Orr, D. J.</creatorcontrib><title>The survival of adult mouse sensory neurons in vitro is enhanced by natural and synthetic substrata, particularly fibronectin</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>Primary cultures derived from adult mouse dorsal root ganglia have been maintained in the presence or absence of 5 × 10 −6M cytosine arabinoside for periods of up to 4 weeks. In cultures in which cytosine arabinoside is present, the non‐neuronal cell population is effectively reduced. When uncoated plastic substrata are used there is also a concurrent decrease in the number of neurons if the medium is supplemented with cytosine arabinoside. The effects on neuron survival of substrata coated with fibronectin, polyornithine, polylysine, and exudates prepared from mouse liver cells were studied. It was shown that neuronal densities similar to those with uninhibited media may be retained in the presence of cytosine arabinoside if fibronectin‐coated substrata are prepared. With the other coating agents neuronal survival was also enhanced but to a lesser extent. The study offers a means therefore of producing purer cultures of dorsal root ganglia neurons than has previously been possible from adult mammalian sources.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>cultured adult neurons</subject><subject>Cytarabine - pharmacology</subject><subject>DRG</subject><subject>fibronectin</subject><subject>Fibronectins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglia, Spinal</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>liver exudates</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>Microscopy, Phase-Contrast</subject><subject>Neurons, Afferent</subject><subject>Peptides</subject><subject>Polylysine</subject><subject>polyornithine</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKtvCkSOSD4hTUxxPYjtHKFBAqyKhQo_WxHG0LllnazsLOfDfMdpoxQlOlma-eZ43j5BnJbsoGeOv7ny4qBpWSgYle0BWJWtkUdWVfEhWDAQrKlbyx-Q0xjvGWNPUcEJOgDdKCFiRXzcbS-MU9m6PAx17it00JLodp5jr1scxzNTbKYw-Uufp3qUwUhep9Rv0xna0zX1MU8jj6DsaZ582NjmTVduYAiY8pzsMuTINGIaZ9q7NatYk55-QRz0O0T5d3jPy9f27m8sPxfrz1cfL1-vCQLZWoBQ1NK1CabHjSrZtb0AAgqwVNrVQUFcopLKgoOdcgO07XhmQokM0CuCMvDzo7sJ4P9mY9NZFY4cBvc1OtZSCAef_B8tKVpUUMoPFATRhjDHYXu-C22KYdcn0n1x0zkUfc8n880V4are2O9JLELn_YuljNDj0IR_XxSOmOJfQlBmTB-yHG-z87z_1p-svfy-wLOxisj-Pkxi-62xH1vr2-krXa3X77Y16qwF-Ay39tvg</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Smith, R. A.</creator><creator>Orr, D. J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>The survival of adult mouse sensory neurons in vitro is enhanced by natural and synthetic substrata, particularly fibronectin</title><author>Smith, R. A. ; Orr, D. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3490-a76539b8a7ead287bbfc363a3758a9568354a678e383f2263efd24c376daac833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>cultured adult neurons</topic><topic>Cytarabine - pharmacology</topic><topic>DRG</topic><topic>fibronectin</topic><topic>Fibronectins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ganglia, Spinal</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>liver exudates</topic><topic>Mice</topic><topic>Mice, Inbred CBA</topic><topic>Microscopy, Phase-Contrast</topic><topic>Neurons, Afferent</topic><topic>Peptides</topic><topic>Polylysine</topic><topic>polyornithine</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, R. A.</creatorcontrib><creatorcontrib>Orr, D. J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, R. A.</au><au>Orr, D. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The survival of adult mouse sensory neurons in vitro is enhanced by natural and synthetic substrata, particularly fibronectin</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J. Neurosci. Res</addtitle><date>1987</date><risdate>1987</risdate><volume>17</volume><issue>3</issue><spage>265</spage><epage>270</epage><pages>265-270</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><coden>JNREDK</coden><abstract>Primary cultures derived from adult mouse dorsal root ganglia have been maintained in the presence or absence of 5 × 10 −6M cytosine arabinoside for periods of up to 4 weeks. In cultures in which cytosine arabinoside is present, the non‐neuronal cell population is effectively reduced. When uncoated plastic substrata are used there is also a concurrent decrease in the number of neurons if the medium is supplemented with cytosine arabinoside. The effects on neuron survival of substrata coated with fibronectin, polyornithine, polylysine, and exudates prepared from mouse liver cells were studied. 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| fulltext | fulltext |
| identifier | ISSN: 0360-4012 |
| ispartof | Journal of neuroscience research, 1987, Vol.17 (3), p.265-270 |
| issn | 0360-4012 1097-4547 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biological and medical sciences Cell Count Cell Survival Cells, Cultured cultured adult neurons Cytarabine - pharmacology DRG fibronectin Fibronectins Fundamental and applied biological sciences. Psychology Ganglia, Spinal Isolated neuron and nerve. Neuroglia liver exudates Mice Mice, Inbred CBA Microscopy, Phase-Contrast Neurons, Afferent Peptides Polylysine polyornithine Vertebrates: nervous system and sense organs |
| title | The survival of adult mouse sensory neurons in vitro is enhanced by natural and synthetic substrata, particularly fibronectin |
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