Evaluation of Diagnostic Tools for Adult Chlamydial Keratoconjunctivitis
Conjunctival smears and serum specimens of 150 patients with presumed chlamydial keratoconjunctivitis were evaluated over a period of 16 months and compared to age- and sex-matched healthy controls. Sensitivity, specificity, and predictive values of fluorescent monoclonal antibody (FMAb) direct test...
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| Veröffentlicht in: | Ophthalmology (Rochester, Minn.) Minn.), 1987-05, Vol.94 (5), p.532-537 |
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| container_end_page | 537 |
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| container_issue | 5 |
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| container_title | Ophthalmology (Rochester, Minn.) |
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| creator | Bialasiewicz, A.A. Jahn, G.J. |
| description | Conjunctival smears and serum specimens of 150 patients with presumed chlamydial keratoconjunctivitis were evaluated over a period of 16 months and compared to age- and sex-matched healthy controls. Sensitivity, specificity, and predictive values of fluorescent monoclonal antibody (FMAb) direct tests, IgG single antigen tests for the detection of serum antibody (Ig-GIFT), and IgA and IgG immunoperoxidase tests (IgA-IPAs and IgG-IPAs) for serum antibody were compared to McCoy cell culture techniques in a nonoptimized clinical setting. Thus, FMAb sensitivity was 100%, specificity was 52%, and predictive value was 30%, IgG-IFT sensitivity was 94%, specificity was 67%, and predictive value was 37%. IgA-IPA sensitivity was 100%, specificity was 70%, and predictive value was 40%. The IgG-IPA was sensitive but nonspecific. With respect to the current epidemiologic situation in our area, FMAb and IgA-IPA in addition to McCoy cell culture techniques may represent valuable diagnostic tools for the detection of chlamydial disease. |
| doi_str_mv | 10.1016/S0161-6420(87)33426-8 |
| format | Article |
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Sensitivity, specificity, and predictive values of fluorescent monoclonal antibody (FMAb) direct tests, IgG single antigen tests for the detection of serum antibody (Ig-GIFT), and IgA and IgG immunoperoxidase tests (IgA-IPAs and IgG-IPAs) for serum antibody were compared to McCoy cell culture techniques in a nonoptimized clinical setting. Thus, FMAb sensitivity was 100%, specificity was 52%, and predictive value was 30%, IgG-IFT sensitivity was 94%, specificity was 67%, and predictive value was 37%. IgA-IPA sensitivity was 100%, specificity was 70%, and predictive value was 40%. The IgG-IPA was sensitive but nonspecific. 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Sensitivity, specificity, and predictive values of fluorescent monoclonal antibody (FMAb) direct tests, IgG single antigen tests for the detection of serum antibody (Ig-GIFT), and IgA and IgG immunoperoxidase tests (IgA-IPAs and IgG-IPAs) for serum antibody were compared to McCoy cell culture techniques in a nonoptimized clinical setting. Thus, FMAb sensitivity was 100%, specificity was 52%, and predictive value was 30%, IgG-IFT sensitivity was 94%, specificity was 67%, and predictive value was 37%. IgA-IPA sensitivity was 100%, specificity was 70%, and predictive value was 40%. The IgG-IPA was sensitive but nonspecific. With respect to the current epidemiologic situation in our area, FMAb and IgA-IPA in addition to McCoy cell culture techniques may represent valuable diagnostic tools for the detection of chlamydial disease.</description><subject>Adult</subject><subject>Bacteriological Techniques</subject><subject>Chlamydia Infections - diagnosis</subject><subject>Chlamydia trachomatis - isolation & purification</subject><subject>chlamydial infections</subject><subject>Conjunctivitis, Inclusion - diagnosis</subject><subject>Conjunctivitis, Inclusion - microbiology</subject><subject>Evaluation Studies as Topic</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>IgA-IPA</subject><subject>IgG-serology</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin A - analysis</subject><subject>Immunoglobulin G - analysis</subject><subject>Infant, Newborn</subject><subject>keratoconjunctivitis</subject><subject>Keratoconjunctivitis - diagnosis</subject><subject>Keratoconjunctivitis - microbiology</subject><subject>Male</subject><subject>McCoy cell culture</subject><subject>monoclonal antibody direct test</subject><subject>Predictive Value of Tests</subject><issn>0161-6420</issn><issn>1549-4713</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EKqXwCZWyQrAI2I4fyQpVpVBEJRaUteXYDrhK4mI7lfr3pA91y2ZmpHtnruYAMEbwAUHEHj_7glJGMLzL-X2WEczS_AwMESVFSjjKzsHwZLkEVyGsIISMZWQABhkuCgzxEMxnG1l3MlrXJq5Knq38bl2IViVL5-qQVM4nE93VMZn-1LLZaivr5N14GZ1y7aprVbQbG224BheVrIO5OfYR-HqZLafzdPHx-jadLFKVMRhTVOUF17TUlBOqcwRLyThE0BCuJcFc695XUEpYiXSV61JiTCTph1LlJSHZCNwe7q69--1MiKKxQZm6lq1xXRCc04IzvDPSg1F5F4I3lVh720i_FQiKHUGxJyh2eETOxZ6gyPu98TGgKxujT1tHZL3-dNBN_-XGGi-CsqZVRltvVBTa2X8S_gB-dICj</recordid><startdate>19870501</startdate><enddate>19870501</enddate><creator>Bialasiewicz, A.A.</creator><creator>Jahn, G.J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870501</creationdate><title>Evaluation of Diagnostic Tools for Adult Chlamydial Keratoconjunctivitis</title><author>Bialasiewicz, A.A. ; Jahn, G.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-1f897d5bd5745d810ba67010e47da427ddc3695546b1df8dba224a48dbbc8b443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Adult</topic><topic>Bacteriological Techniques</topic><topic>Chlamydia Infections - diagnosis</topic><topic>Chlamydia trachomatis - isolation & purification</topic><topic>chlamydial infections</topic><topic>Conjunctivitis, Inclusion - diagnosis</topic><topic>Conjunctivitis, Inclusion - microbiology</topic><topic>Evaluation Studies as Topic</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>IgA-IPA</topic><topic>IgG-serology</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin A - analysis</topic><topic>Immunoglobulin G - analysis</topic><topic>Infant, Newborn</topic><topic>keratoconjunctivitis</topic><topic>Keratoconjunctivitis - diagnosis</topic><topic>Keratoconjunctivitis - microbiology</topic><topic>Male</topic><topic>McCoy cell culture</topic><topic>monoclonal antibody direct test</topic><topic>Predictive Value of Tests</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bialasiewicz, A.A.</creatorcontrib><creatorcontrib>Jahn, G.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ophthalmology (Rochester, Minn.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bialasiewicz, A.A.</au><au>Jahn, G.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Diagnostic Tools for Adult Chlamydial Keratoconjunctivitis</atitle><jtitle>Ophthalmology (Rochester, Minn.)</jtitle><addtitle>Ophthalmology</addtitle><date>1987-05-01</date><risdate>1987</risdate><volume>94</volume><issue>5</issue><spage>532</spage><epage>537</epage><pages>532-537</pages><issn>0161-6420</issn><eissn>1549-4713</eissn><abstract>Conjunctival smears and serum specimens of 150 patients with presumed chlamydial keratoconjunctivitis were evaluated over a period of 16 months and compared to age- and sex-matched healthy controls. Sensitivity, specificity, and predictive values of fluorescent monoclonal antibody (FMAb) direct tests, IgG single antigen tests for the detection of serum antibody (Ig-GIFT), and IgA and IgG immunoperoxidase tests (IgA-IPAs and IgG-IPAs) for serum antibody were compared to McCoy cell culture techniques in a nonoptimized clinical setting. Thus, FMAb sensitivity was 100%, specificity was 52%, and predictive value was 30%, IgG-IFT sensitivity was 94%, specificity was 67%, and predictive value was 37%. IgA-IPA sensitivity was 100%, specificity was 70%, and predictive value was 40%. The IgG-IPA was sensitive but nonspecific. With respect to the current epidemiologic situation in our area, FMAb and IgA-IPA in addition to McCoy cell culture techniques may represent valuable diagnostic tools for the detection of chlamydial disease.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>3299202</pmid><doi>10.1016/S0161-6420(87)33426-8</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0161-6420 |
| ispartof | Ophthalmology (Rochester, Minn.), 1987-05, Vol.94 (5), p.532-537 |
| issn | 0161-6420 1549-4713 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Bacteriological Techniques Chlamydia Infections - diagnosis Chlamydia trachomatis - isolation & purification chlamydial infections Conjunctivitis, Inclusion - diagnosis Conjunctivitis, Inclusion - microbiology Evaluation Studies as Topic Fluorescent Antibody Technique Humans IgA-IPA IgG-serology Immunoenzyme Techniques Immunoglobulin A - analysis Immunoglobulin G - analysis Infant, Newborn keratoconjunctivitis Keratoconjunctivitis - diagnosis Keratoconjunctivitis - microbiology Male McCoy cell culture monoclonal antibody direct test Predictive Value of Tests |
| title | Evaluation of Diagnostic Tools for Adult Chlamydial Keratoconjunctivitis |
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