Decreased ratio of α- to β-globin mRNA in reticulocyte membrane RNA
1. 1. The amount of RNA obtained from rabbit reticulocyte membrane-bound ribosomes by direct phenol extraction of washed membranes was inversely related to the hematocrit of the animals. 2. 2. Translation of the RNA in the reticulocyte translation system showed that the M r = 30,000 protein reported...
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| Veröffentlicht in: | International journal of biochemistry 1987, Vol.19 (6), p.523-529 |
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| container_issue | 6 |
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| container_title | International journal of biochemistry |
| container_volume | 19 |
| creator | Mezl, Vasek A. Cutcliffe, Nora |
| description | 1.
1. The amount of RNA obtained from rabbit reticulocyte membrane-bound ribosomes by direct phenol extraction of washed membranes was inversely related to the hematocrit of the animals.
2.
2. Translation of the RNA in the reticulocyte translation system showed that the
M
r = 30,000 protein reported to be a marker of membrane polysomes was also made by an endogenous mRNA in this translation system.
3.
3. Analyses of the translation products made in the wheat germ system on Triton X-100 acid urea gels show that membrane RNAs display a characteristic α- to β-globin ratio of 0.77 which differentiates them from RNAs prepared from cytoplasmic polysomes and from the postpolysomal supernatant.
4.
4. These results show that free and membrane-bound ribosomes can be distinguished by the main protein that they produce. |
| doi_str_mv | 10.1016/0020-711X(87)90136-4 |
| format | Article |
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1. The amount of RNA obtained from rabbit reticulocyte membrane-bound ribosomes by direct phenol extraction of washed membranes was inversely related to the hematocrit of the animals.
2.
2. Translation of the RNA in the reticulocyte translation system showed that the
M
r = 30,000 protein reported to be a marker of membrane polysomes was also made by an endogenous mRNA in this translation system.
3.
3. Analyses of the translation products made in the wheat germ system on Triton X-100 acid urea gels show that membrane RNAs display a characteristic α- to β-globin ratio of 0.77 which differentiates them from RNAs prepared from cytoplasmic polysomes and from the postpolysomal supernatant.
4.
4. These results show that free and membrane-bound ribosomes can be distinguished by the main protein that they produce.</description><identifier>ISSN: 0020-711X</identifier><identifier>DOI: 10.1016/0020-711X(87)90136-4</identifier><identifier>PMID: 3609443</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Cell Membrane - metabolism ; Cytoplasm - metabolism ; Globins - genetics ; Polyribosomes - metabolism ; Protein Biosynthesis ; Rabbits ; Reticulocytes - metabolism ; RNA, Messenger - metabolism</subject><ispartof>International journal of biochemistry, 1987, Vol.19 (6), p.523-529</ispartof><rights>1987</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c252t-f541b2c62b8f2a47bfd4bb73ef202a19d0711b327a11c51dbe3545c286e9a76a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3609443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mezl, Vasek A.</creatorcontrib><creatorcontrib>Cutcliffe, Nora</creatorcontrib><title>Decreased ratio of α- to β-globin mRNA in reticulocyte membrane RNA</title><title>International journal of biochemistry</title><addtitle>Int J Biochem</addtitle><description>1.
1. The amount of RNA obtained from rabbit reticulocyte membrane-bound ribosomes by direct phenol extraction of washed membranes was inversely related to the hematocrit of the animals.
2.
2. Translation of the RNA in the reticulocyte translation system showed that the
M
r = 30,000 protein reported to be a marker of membrane polysomes was also made by an endogenous mRNA in this translation system.
3.
3. Analyses of the translation products made in the wheat germ system on Triton X-100 acid urea gels show that membrane RNAs display a characteristic α- to β-globin ratio of 0.77 which differentiates them from RNAs prepared from cytoplasmic polysomes and from the postpolysomal supernatant.
4.
4. These results show that free and membrane-bound ribosomes can be distinguished by the main protein that they produce.</description><subject>Animals</subject><subject>Cell Membrane - metabolism</subject><subject>Cytoplasm - metabolism</subject><subject>Globins - genetics</subject><subject>Polyribosomes - metabolism</subject><subject>Protein Biosynthesis</subject><subject>Rabbits</subject><subject>Reticulocytes - metabolism</subject><subject>RNA, Messenger - metabolism</subject><issn>0020-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1KxDAUhbNQRh19A4WsRBfV3DRt2o0g4y8MCqLgLiTprUTayZi0go-lD-Iz2XGGWerqwD3nnnv5CNkHdgIM8lPGOEskwPNRIY9LBmmeiA2yvR5vkZ0YXxmDshAwIqM0Z6UQ6Ta5vEAbUEesaNCd89TX9PszoZ2n31_JS-ONm9H24e6cDhqwc7ZvvP3okLbYmqBnSAdzl2zWuom4t9Ixebq6fJzcJNP769vJ-TSxPONdUmcCDLc5N0XNtZCmroQxMsWaM66hrNjwq0m51AA2g8pgmonM8iLHUstcp2NyuOydB__WY-xU66LFphn-8H1UUmalhJL9G4QMIGeFGIJiGbTBxxiwVvPgWh0-FDC1QKsWDNWCoSqk-kWrFmsHq_7etFitl1ZcB_9s6eNA491hUNE6nFmsXEDbqcq7vw_8AGbjick</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Mezl, Vasek A.</creator><creator>Cutcliffe, Nora</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Decreased ratio of α- to β-globin mRNA in reticulocyte membrane RNA</title><author>Mezl, Vasek A. ; Cutcliffe, Nora</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c252t-f541b2c62b8f2a47bfd4bb73ef202a19d0711b327a11c51dbe3545c286e9a76a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Cell Membrane - metabolism</topic><topic>Cytoplasm - metabolism</topic><topic>Globins - genetics</topic><topic>Polyribosomes - metabolism</topic><topic>Protein Biosynthesis</topic><topic>Rabbits</topic><topic>Reticulocytes - metabolism</topic><topic>RNA, Messenger - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Mezl, Vasek A.</creatorcontrib><creatorcontrib>Cutcliffe, Nora</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mezl, Vasek A.</au><au>Cutcliffe, Nora</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased ratio of α- to β-globin mRNA in reticulocyte membrane RNA</atitle><jtitle>International journal of biochemistry</jtitle><addtitle>Int J Biochem</addtitle><date>1987</date><risdate>1987</risdate><volume>19</volume><issue>6</issue><spage>523</spage><epage>529</epage><pages>523-529</pages><issn>0020-711X</issn><abstract>1.
1. The amount of RNA obtained from rabbit reticulocyte membrane-bound ribosomes by direct phenol extraction of washed membranes was inversely related to the hematocrit of the animals.
2.
2. Translation of the RNA in the reticulocyte translation system showed that the
M
r = 30,000 protein reported to be a marker of membrane polysomes was also made by an endogenous mRNA in this translation system.
3.
3. Analyses of the translation products made in the wheat germ system on Triton X-100 acid urea gels show that membrane RNAs display a characteristic α- to β-globin ratio of 0.77 which differentiates them from RNAs prepared from cytoplasmic polysomes and from the postpolysomal supernatant.
4.
4. These results show that free and membrane-bound ribosomes can be distinguished by the main protein that they produce.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>3609443</pmid><doi>10.1016/0020-711X(87)90136-4</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | International journal of biochemistry, 1987, Vol.19 (6), p.523-529 |
| issn | 0020-711X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Membrane - metabolism Cytoplasm - metabolism Globins - genetics Polyribosomes - metabolism Protein Biosynthesis Rabbits Reticulocytes - metabolism RNA, Messenger - metabolism |
| title | Decreased ratio of α- to β-globin mRNA in reticulocyte membrane RNA |
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