Immunohistochemical identification of proteoglycan form of macrophage colony-stimulating factor on bone surface
Several studies using op/op mice have shown that macrophage colony-stimulating factor (M-CSF) was necessary for osteoclast formation in vivo. Previously we reported that osteoblastic cells produced two molecular forms of M-CSF; one is an 85-kDa M-CSF, and the other is a proteoglycan form of M-CSF (P...
Gespeichert in:
| Veröffentlicht in: | Calcified tissue international 1995-09, Vol.57 (3), p.213-217 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 217 |
|---|---|
| container_issue | 3 |
| container_start_page | 213 |
| container_title | Calcified tissue international |
| container_volume | 57 |
| creator | Ohtsuki, T Hatake, K Suzu, S Saito, K Motoyoshi, K Miura, Y |
| description | Several studies using op/op mice have shown that macrophage colony-stimulating factor (M-CSF) was necessary for osteoclast formation in vivo. Previously we reported that osteoblastic cells produced two molecular forms of M-CSF; one is an 85-kDa M-CSF, and the other is a proteoglycan form of M-CSF (PG-M-CSF) which has a binding affinity for bone-derived collagens and is extractable from human bone. In this study, we performed immunostaining of human bone using a newly established anti-PG-M-CSF antibody, and showed positive staining PG-M-CSF, probably produced by bone lining cells, on the bone surface. This observation suggests that the bone surface is suitable for osteoclast formation because of the presence of PG-M-CSF. |
| doi_str_mv | 10.1007/BF00310261 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77594775</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>77594775</sourcerecordid><originalsourceid>FETCH-LOGICAL-c313t-1fccfb0a208abae9fa18d84b80508698d192322b2294f8f43d99a5f243cdb9973</originalsourceid><addsrcrecordid>eNqFkT1PwzAQhi0EKqWwsCNlYkAKnD_i2CNUFCpVYgGJLXIcuw2K4xI7Q_89rlrByHJ3Oj336u5ehK4x3GOA8uFpAUAxEI5P0BQzSnIQpDxFU8AlziUvP8_RRQhfAJhxzidoIoqSSSqnyC-dG3u_aUP0emNcq1WXtY3pY2tTHVvfZ95m28FH49fdTqs-s35w-6ZTevDbjVqbTPvO97s8xNaNXZrq15lVOvohS_O1700WxiF1zCU6s6oL5uqYZ-hj8fw-f81Xby_L-eMq1xTTmGOrta1BERCqVkZahUUjWC2gAMGlaLAklJCaEMmssIw2UqrCEkZ1U0tZ0hm6Peimzb9HE2Ll2qBN16ne-DFUZVlIlsK_IOaiACZ5Au8OYDo6hMHYaju0Tg27CkO1t6H6syHBN0fVsXam-UWPf6c_6tiEdQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16850496</pqid></control><display><type>article</type><title>Immunohistochemical identification of proteoglycan form of macrophage colony-stimulating factor on bone surface</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Ohtsuki, T ; Hatake, K ; Suzu, S ; Saito, K ; Motoyoshi, K ; Miura, Y</creator><creatorcontrib>Ohtsuki, T ; Hatake, K ; Suzu, S ; Saito, K ; Motoyoshi, K ; Miura, Y</creatorcontrib><description>Several studies using op/op mice have shown that macrophage colony-stimulating factor (M-CSF) was necessary for osteoclast formation in vivo. Previously we reported that osteoblastic cells produced two molecular forms of M-CSF; one is an 85-kDa M-CSF, and the other is a proteoglycan form of M-CSF (PG-M-CSF) which has a binding affinity for bone-derived collagens and is extractable from human bone. In this study, we performed immunostaining of human bone using a newly established anti-PG-M-CSF antibody, and showed positive staining PG-M-CSF, probably produced by bone lining cells, on the bone surface. This observation suggests that the bone surface is suitable for osteoclast formation because of the presence of PG-M-CSF.</description><identifier>ISSN: 0171-967X</identifier><identifier>EISSN: 1432-0827</identifier><identifier>DOI: 10.1007/BF00310261</identifier><identifier>PMID: 8574939</identifier><language>eng</language><publisher>United States</publisher><subject>Aged ; Amino Acid Sequence ; Animals ; Antibodies, Monoclonal ; Bone and Bones - metabolism ; CHO Cells ; Cricetinae ; Humans ; Immunohistochemistry ; Macrophage Colony-Stimulating Factor - immunology ; Macrophage Colony-Stimulating Factor - metabolism ; Male ; Mice ; Molecular Sequence Data ; Proteoglycans - metabolism ; Rabbits</subject><ispartof>Calcified tissue international, 1995-09, Vol.57 (3), p.213-217</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-1fccfb0a208abae9fa18d84b80508698d192322b2294f8f43d99a5f243cdb9973</citedby><cites>FETCH-LOGICAL-c313t-1fccfb0a208abae9fa18d84b80508698d192322b2294f8f43d99a5f243cdb9973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8574939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohtsuki, T</creatorcontrib><creatorcontrib>Hatake, K</creatorcontrib><creatorcontrib>Suzu, S</creatorcontrib><creatorcontrib>Saito, K</creatorcontrib><creatorcontrib>Motoyoshi, K</creatorcontrib><creatorcontrib>Miura, Y</creatorcontrib><title>Immunohistochemical identification of proteoglycan form of macrophage colony-stimulating factor on bone surface</title><title>Calcified tissue international</title><addtitle>Calcif Tissue Int</addtitle><description>Several studies using op/op mice have shown that macrophage colony-stimulating factor (M-CSF) was necessary for osteoclast formation in vivo. Previously we reported that osteoblastic cells produced two molecular forms of M-CSF; one is an 85-kDa M-CSF, and the other is a proteoglycan form of M-CSF (PG-M-CSF) which has a binding affinity for bone-derived collagens and is extractable from human bone. In this study, we performed immunostaining of human bone using a newly established anti-PG-M-CSF antibody, and showed positive staining PG-M-CSF, probably produced by bone lining cells, on the bone surface. This observation suggests that the bone surface is suitable for osteoclast formation because of the presence of PG-M-CSF.</description><subject>Aged</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Bone and Bones - metabolism</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Macrophage Colony-Stimulating Factor - immunology</subject><subject>Macrophage Colony-Stimulating Factor - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Proteoglycans - metabolism</subject><subject>Rabbits</subject><issn>0171-967X</issn><issn>1432-0827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1PwzAQhi0EKqWwsCNlYkAKnD_i2CNUFCpVYgGJLXIcuw2K4xI7Q_89rlrByHJ3Oj336u5ehK4x3GOA8uFpAUAxEI5P0BQzSnIQpDxFU8AlziUvP8_RRQhfAJhxzidoIoqSSSqnyC-dG3u_aUP0emNcq1WXtY3pY2tTHVvfZ95m28FH49fdTqs-s35w-6ZTevDbjVqbTPvO97s8xNaNXZrq15lVOvohS_O1700WxiF1zCU6s6oL5uqYZ-hj8fw-f81Xby_L-eMq1xTTmGOrta1BERCqVkZahUUjWC2gAMGlaLAklJCaEMmssIw2UqrCEkZ1U0tZ0hm6Peimzb9HE2Ll2qBN16ne-DFUZVlIlsK_IOaiACZ5Au8OYDo6hMHYaju0Tg27CkO1t6H6syHBN0fVsXam-UWPf6c_6tiEdQ</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>Ohtsuki, T</creator><creator>Hatake, K</creator><creator>Suzu, S</creator><creator>Saito, K</creator><creator>Motoyoshi, K</creator><creator>Miura, Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19950901</creationdate><title>Immunohistochemical identification of proteoglycan form of macrophage colony-stimulating factor on bone surface</title><author>Ohtsuki, T ; Hatake, K ; Suzu, S ; Saito, K ; Motoyoshi, K ; Miura, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-1fccfb0a208abae9fa18d84b80508698d192322b2294f8f43d99a5f243cdb9973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Aged</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Bone and Bones - metabolism</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Macrophage Colony-Stimulating Factor - immunology</topic><topic>Macrophage Colony-Stimulating Factor - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Proteoglycans - metabolism</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohtsuki, T</creatorcontrib><creatorcontrib>Hatake, K</creatorcontrib><creatorcontrib>Suzu, S</creatorcontrib><creatorcontrib>Saito, K</creatorcontrib><creatorcontrib>Motoyoshi, K</creatorcontrib><creatorcontrib>Miura, Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Calcified tissue international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohtsuki, T</au><au>Hatake, K</au><au>Suzu, S</au><au>Saito, K</au><au>Motoyoshi, K</au><au>Miura, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical identification of proteoglycan form of macrophage colony-stimulating factor on bone surface</atitle><jtitle>Calcified tissue international</jtitle><addtitle>Calcif Tissue Int</addtitle><date>1995-09-01</date><risdate>1995</risdate><volume>57</volume><issue>3</issue><spage>213</spage><epage>217</epage><pages>213-217</pages><issn>0171-967X</issn><eissn>1432-0827</eissn><abstract>Several studies using op/op mice have shown that macrophage colony-stimulating factor (M-CSF) was necessary for osteoclast formation in vivo. Previously we reported that osteoblastic cells produced two molecular forms of M-CSF; one is an 85-kDa M-CSF, and the other is a proteoglycan form of M-CSF (PG-M-CSF) which has a binding affinity for bone-derived collagens and is extractable from human bone. In this study, we performed immunostaining of human bone using a newly established anti-PG-M-CSF antibody, and showed positive staining PG-M-CSF, probably produced by bone lining cells, on the bone surface. This observation suggests that the bone surface is suitable for osteoclast formation because of the presence of PG-M-CSF.</abstract><cop>United States</cop><pmid>8574939</pmid><doi>10.1007/BF00310261</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0171-967X |
| ispartof | Calcified tissue international, 1995-09, Vol.57 (3), p.213-217 |
| issn | 0171-967X 1432-0827 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77594775 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Aged Amino Acid Sequence Animals Antibodies, Monoclonal Bone and Bones - metabolism CHO Cells Cricetinae Humans Immunohistochemistry Macrophage Colony-Stimulating Factor - immunology Macrophage Colony-Stimulating Factor - metabolism Male Mice Molecular Sequence Data Proteoglycans - metabolism Rabbits |
| title | Immunohistochemical identification of proteoglycan form of macrophage colony-stimulating factor on bone surface |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T18%3A30%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunohistochemical%20identification%20of%20proteoglycan%20form%20of%20macrophage%20colony-stimulating%20factor%20on%20bone%20surface&rft.jtitle=Calcified%20tissue%20international&rft.au=Ohtsuki,%20T&rft.date=1995-09-01&rft.volume=57&rft.issue=3&rft.spage=213&rft.epage=217&rft.pages=213-217&rft.issn=0171-967X&rft.eissn=1432-0827&rft_id=info:doi/10.1007/BF00310261&rft_dat=%3Cproquest_cross%3E77594775%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16850496&rft_id=info:pmid/8574939&rfr_iscdi=true |