Quantitation of C-reactive protein in cerebrospinal fluid and serum by zone immunoelectrophoresis assay (ZIA)
The zone immunoelectrophoresis assay (ZIA) for C-reactive protein (CRP) determinations is easy to perform and requires only small amount of antiserum, e.g., 25–100 and 0.5–1.0 μ1 anti-CRP antibody/20 serum and CSF samples, respectively. For quantitating CSF-CRP the immunoprecipitates formed were sta...
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| Veröffentlicht in: | Journal of immunological methods 1987-06, Vol.100 (1), p.191-195 |
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| creator | Hansson, L.-O. Lindquist, L. Linné, T. Sego, E. |
| description | The zone immunoelectrophoresis assay (ZIA) for C-reactive protein (CRP) determinations is easy to perform and requires only small amount of antiserum, e.g., 25–100 and 0.5–1.0 μ1 anti-CRP antibody/20 serum and CSF samples, respectively. For quantitating CSF-CRP the immunoprecipitates formed were stained using lakaline phosphatase-conjugated secondary antibodies and the lowest standard concentration used was 30 μg/1. The immunoprecipitates formed when measuring CRP in serum were stained by Coomasie brilliant blue R250 with a detection limit of about 300 μg/l.
CRP was determined in cerebrospinal fluid in 27 patients with bacterial meningitis (range |
| doi_str_mv | 10.1016/0022-1759(87)90189-X |
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CRP was determined in cerebrospinal fluid in 27 patients with bacterial meningitis (range <0.03–0.23 mg/l) and in 25 patients with viral meningitis (range <0.03–0.23 mg/l).
CRP was quantitated in 52 sera by both the CRP ZIA method (
y) and by electroimmunoassay (
x). The correlation coefficient was
r = 0.992 with the regression line
y = 1.024
x + 0.855.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(87)90189-X</identifier><identifier>PMID: 3598196</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; C-Reactive Protein - analysis ; C-Reactive Protein - cerebrospinal fluid ; C-reative protein ; Cerebrospinal fluid ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunoelectrophoresis ; Meningitis ; Meningitis - cerebrospinal fluid ; Molecular immunology ; Techniques</subject><ispartof>Journal of immunological methods, 1987-06, Vol.100 (1), p.191-195</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-53aad3f58a976bc2f7aa4900e7b33e0f0fdd0c35334ca9c81794dbe52e53fe323</citedby><cites>FETCH-LOGICAL-c386t-53aad3f58a976bc2f7aa4900e7b33e0f0fdd0c35334ca9c81794dbe52e53fe323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(87)90189-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7402648$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3598196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hansson, L.-O.</creatorcontrib><creatorcontrib>Lindquist, L.</creatorcontrib><creatorcontrib>Linné, T.</creatorcontrib><creatorcontrib>Sego, E.</creatorcontrib><title>Quantitation of C-reactive protein in cerebrospinal fluid and serum by zone immunoelectrophoresis assay (ZIA)</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>The zone immunoelectrophoresis assay (ZIA) for C-reactive protein (CRP) determinations is easy to perform and requires only small amount of antiserum, e.g., 25–100 and 0.5–1.0 μ1 anti-CRP antibody/20 serum and CSF samples, respectively. For quantitating CSF-CRP the immunoprecipitates formed were stained using lakaline phosphatase-conjugated secondary antibodies and the lowest standard concentration used was 30 μg/1. The immunoprecipitates formed when measuring CRP in serum were stained by Coomasie brilliant blue R250 with a detection limit of about 300 μg/l.
CRP was determined in cerebrospinal fluid in 27 patients with bacterial meningitis (range <0.03–0.23 mg/l) and in 25 patients with viral meningitis (range <0.03–0.23 mg/l).
CRP was quantitated in 52 sera by both the CRP ZIA method (
y) and by electroimmunoassay (
x). The correlation coefficient was
r = 0.992 with the regression line
y = 1.024
x + 0.855.</description><subject>Biological and medical sciences</subject><subject>C-Reactive Protein - analysis</subject><subject>C-Reactive Protein - cerebrospinal fluid</subject><subject>C-reative protein</subject><subject>Cerebrospinal fluid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunoelectrophoresis</subject><subject>Meningitis</subject><subject>Meningitis - cerebrospinal fluid</subject><subject>Molecular immunology</subject><subject>Techniques</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo67j6DxRyENk9tCad7k5yEZbBj4UFERQWL6E6qWCkOxmT7oXx15txhjkKBXWop4p6H0JecvaWMz68Y6xtGy57faXktWZc6eb-EdlwJdtGatY_Jpsz8pQ8K-UXY4yzgV2QC9FrxfWwIfPXFeISFlhCijR5um0ygl3CA9JdTguGSGtZzDjmVHYhwkT9tAZHITpaMK8zHff0T4pIwzyvMeGEdslp9zNlLKFQKAX29OrH7c31c_LEw1Twxalfku8fP3zbfm7uvny63d7cNVaoYWl6AeCE7xVoOYy29RKg04yhHIVA5pl3jlnRC9FZ0FZxqTs3Yt9iLzyKVlySN8e7NcLvFcti5lAsThNETGsxsipph4FXsDuCtoYrGb3Z5TBD3hvOzMGyOSg0B4VGSfPPsrmva69O99dxRndeOmmt89enORQLk88QbShnTHasHTpVsfdHDKuLh4DZFBswWnQhV4fGpfD_P_4COG2aog</recordid><startdate>19870626</startdate><enddate>19870626</enddate><creator>Hansson, L.-O.</creator><creator>Lindquist, L.</creator><creator>Linné, T.</creator><creator>Sego, E.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870626</creationdate><title>Quantitation of C-reactive protein in cerebrospinal fluid and serum by zone immunoelectrophoresis assay (ZIA)</title><author>Hansson, L.-O. ; Lindquist, L. ; Linné, T. ; Sego, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-53aad3f58a976bc2f7aa4900e7b33e0f0fdd0c35334ca9c81794dbe52e53fe323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>C-Reactive Protein - analysis</topic><topic>C-Reactive Protein - cerebrospinal fluid</topic><topic>C-reative protein</topic><topic>Cerebrospinal fluid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunoelectrophoresis</topic><topic>Meningitis</topic><topic>Meningitis - cerebrospinal fluid</topic><topic>Molecular immunology</topic><topic>Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hansson, L.-O.</creatorcontrib><creatorcontrib>Lindquist, L.</creatorcontrib><creatorcontrib>Linné, T.</creatorcontrib><creatorcontrib>Sego, E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hansson, L.-O.</au><au>Lindquist, L.</au><au>Linné, T.</au><au>Sego, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitation of C-reactive protein in cerebrospinal fluid and serum by zone immunoelectrophoresis assay (ZIA)</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1987-06-26</date><risdate>1987</risdate><volume>100</volume><issue>1</issue><spage>191</spage><epage>195</epage><pages>191-195</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>The zone immunoelectrophoresis assay (ZIA) for C-reactive protein (CRP) determinations is easy to perform and requires only small amount of antiserum, e.g., 25–100 and 0.5–1.0 μ1 anti-CRP antibody/20 serum and CSF samples, respectively. For quantitating CSF-CRP the immunoprecipitates formed were stained using lakaline phosphatase-conjugated secondary antibodies and the lowest standard concentration used was 30 μg/1. The immunoprecipitates formed when measuring CRP in serum were stained by Coomasie brilliant blue R250 with a detection limit of about 300 μg/l.
CRP was determined in cerebrospinal fluid in 27 patients with bacterial meningitis (range <0.03–0.23 mg/l) and in 25 patients with viral meningitis (range <0.03–0.23 mg/l).
CRP was quantitated in 52 sera by both the CRP ZIA method (
y) and by electroimmunoassay (
x). The correlation coefficient was
r = 0.992 with the regression line
y = 1.024
x + 0.855.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3598196</pmid><doi>10.1016/0022-1759(87)90189-X</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1987-06, Vol.100 (1), p.191-195 |
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| language | eng |
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| subjects | Biological and medical sciences C-Reactive Protein - analysis C-Reactive Protein - cerebrospinal fluid C-reative protein Cerebrospinal fluid Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunoelectrophoresis Meningitis Meningitis - cerebrospinal fluid Molecular immunology Techniques |
| title | Quantitation of C-reactive protein in cerebrospinal fluid and serum by zone immunoelectrophoresis assay (ZIA) |
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