Characterization of a Novel Zinc Finger Gene (ZNF165) Mapping to 6p21 That Is Expressed Specifically in Testis
Clone ZF388 was identified during the course of expressed sequence tag analysis of human adult testis cDNAs as being a member of the C2H2 zinc finger gene family. Northern blot analysis of a range of 16 different human tissues has shown that clone ZF388 detects a single 2.2-kb transcript and that th...
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| Veröffentlicht in: | Genomics 1995-08, Vol.28 (3), p.485-490 |
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| creator | Tirosvoutis, Konstantinos N. Divane, Aspasia Jones, Michael Affara, Nabeel A. |
| description | Clone ZF388 was identified during the course of expressed sequence tag analysis of human adult testis cDNAs as being a member of the C2H2 zinc finger gene family. Northern blot analysis of a range of 16 different human tissues has shown that clone ZF388 detects a single 2.2-kb transcript and that the expression of this mRNA species is strictly specific to the testis. Sequence analysis of ZF388 and other clones isolated by rescreening a testis cDNA library has defined a transcript with an extensive N-terminal acidic domain containing 17% aspartic and glutamic acid residues and a C-terminal domain composed of five zinc finger motifs linked through the highly conserved sequence TGE-KPYE. This gene has been designated ZNF165. Analysis of somatic cell hybrids containing single human chromosomes shows that ZNF165 maps to chromosome 6. Fluorescence
in situ hybridization of cDNA insert probes to male metaphase spreads shows that the ZNF165 transcript maps to 6p21 close to the human MHC region. Human 6p21 is homologous with the distal inversion of the mouse t-complex, which has been shown to contain a cluster of genes expressed specifically in the testis. |
| doi_str_mv | 10.1006/geno.1995.1178 |
| format | Article |
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in situ hybridization of cDNA insert probes to male metaphase spreads shows that the ZNF165 transcript maps to 6p21 close to the human MHC region. Human 6p21 is homologous with the distal inversion of the mouse t-complex, which has been shown to contain a cluster of genes expressed specifically in the testis.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1995.1178</identifier><identifier>PMID: 7490084</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adult ; Animals ; Base Sequence ; Biological and medical sciences ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Chromosome Mapping ; Chromosomes, Human, Pair 6 ; Cloning, Molecular ; Cricetinae ; DNA - analysis ; DNA HYBRIDIZATION ; DNA Primers ; DNA SEQUENCING ; DNA-Binding Proteins - genetics ; DNA-CLONING ; FLUORESCENCE ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; GENE REGULATION ; GENES ; Genes. Genome ; GENETIC MAPPING ; HISTOCOMPATIBILITY COMPLEX ; HUMAN CHROMOSOME 6 ; Humans ; HYBRIDIZATION ; Male ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; PROBES ; PROTEINS ; SOMATIC CELLS ; TESTES ; TRANSCRIPTION ; Zinc Fingers - genetics</subject><ispartof>Genomics, 1995-08, Vol.28 (3), p.485-490</ispartof><rights>1995 Academic Press</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-12adc2c158bd2283d813747dcbef15e4227736b1fe344d3d0f64c0155b3a259f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754385711780$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3638029$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7490084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/390671$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Tirosvoutis, Konstantinos N.</creatorcontrib><creatorcontrib>Divane, Aspasia</creatorcontrib><creatorcontrib>Jones, Michael</creatorcontrib><creatorcontrib>Affara, Nabeel A.</creatorcontrib><title>Characterization of a Novel Zinc Finger Gene (ZNF165) Mapping to 6p21 That Is Expressed Specifically in Testis</title><title>Genomics</title><addtitle>Genomics</addtitle><description>Clone ZF388 was identified during the course of expressed sequence tag analysis of human adult testis cDNAs as being a member of the C2H2 zinc finger gene family. Northern blot analysis of a range of 16 different human tissues has shown that clone ZF388 detects a single 2.2-kb transcript and that the expression of this mRNA species is strictly specific to the testis. Sequence analysis of ZF388 and other clones isolated by rescreening a testis cDNA library has defined a transcript with an extensive N-terminal acidic domain containing 17% aspartic and glutamic acid residues and a C-terminal domain composed of five zinc finger motifs linked through the highly conserved sequence TGE-KPYE. This gene has been designated ZNF165. Analysis of somatic cell hybrids containing single human chromosomes shows that ZNF165 maps to chromosome 6. Fluorescence
in situ hybridization of cDNA insert probes to male metaphase spreads shows that the ZNF165 transcript maps to 6p21 close to the human MHC region. Human 6p21 is homologous with the distal inversion of the mouse t-complex, which has been shown to contain a cluster of genes expressed specifically in the testis.</description><subject>Adult</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 6</subject><subject>Cloning, Molecular</subject><subject>Cricetinae</subject><subject>DNA - analysis</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA Primers</subject><subject>DNA SEQUENCING</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-CLONING</subject><subject>FLUORESCENCE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>GENE REGULATION</subject><subject>GENES</subject><subject>Genes. Genome</subject><subject>GENETIC MAPPING</subject><subject>HISTOCOMPATIBILITY COMPLEX</subject><subject>HUMAN CHROMOSOME 6</subject><subject>Humans</subject><subject>HYBRIDIZATION</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>PROBES</subject><subject>PROTEINS</subject><subject>SOMATIC CELLS</subject><subject>TESTES</subject><subject>TRANSCRIPTION</subject><subject>Zinc Fingers - genetics</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctvEzEQxi0EKqFw5YZkJITgsMGv9eOIoqZUKuVAuPRiOfZsY7SxF3tTUf56dknUG6eRZn7z-j6EXlOypITIT3eQ8pIa0y4pVfoJWlCiTaOlkE_RgmitG9UK_hy9qPUnIcRwzc7QmRKGEC0WKK12rjg_Qol_3BhzwrnDDt_ke-jxbUwer2O6g4IvIQH-cHuzprL9iL-6YZjyeMxYDozizc6N-Krii99DgVoh4O8D-NhF7_r-AceEN1DHWF-iZ53rK7w6xXP0Y32xWX1prr9dXq0-XzeeGzE2lLngmaet3gbGNA-aciVU8FvoaAuCMaW43NIOuBCBB9JJ4Qlt2y13rDUdP0dvj3PztNVWH0fwO59TAj9abohUdGLeH5mh5F-H6Ty7j9VD37sE-VCtUq1hkpkJXB5BX3KtBTo7lLh35cFSYmcT7GyCnU2wswlTw5vT5MN2D-ERP6k-1d-d6q5OAnXFJR_rI8Yl1-TfXn3EYFLqPkKZH4HkIcQy_xFy_N8FfwG6wqBf</recordid><startdate>19950810</startdate><enddate>19950810</enddate><creator>Tirosvoutis, Konstantinos N.</creator><creator>Divane, Aspasia</creator><creator>Jones, Michael</creator><creator>Affara, Nabeel A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19950810</creationdate><title>Characterization of a Novel Zinc Finger Gene (ZNF165) Mapping to 6p21 That Is Expressed Specifically in Testis</title><author>Tirosvoutis, Konstantinos N. ; Divane, Aspasia ; Jones, Michael ; Affara, Nabeel A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-12adc2c158bd2283d813747dcbef15e4227736b1fe344d3d0f64c0155b3a259f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 6</topic><topic>Cloning, Molecular</topic><topic>Cricetinae</topic><topic>DNA - analysis</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA Primers</topic><topic>DNA SEQUENCING</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-CLONING</topic><topic>FLUORESCENCE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>GENE REGULATION</topic><topic>GENES</topic><topic>Genes. Genome</topic><topic>GENETIC MAPPING</topic><topic>HISTOCOMPATIBILITY COMPLEX</topic><topic>HUMAN CHROMOSOME 6</topic><topic>Humans</topic><topic>HYBRIDIZATION</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>PROBES</topic><topic>PROTEINS</topic><topic>SOMATIC CELLS</topic><topic>TESTES</topic><topic>TRANSCRIPTION</topic><topic>Zinc Fingers - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tirosvoutis, Konstantinos N.</creatorcontrib><creatorcontrib>Divane, Aspasia</creatorcontrib><creatorcontrib>Jones, Michael</creatorcontrib><creatorcontrib>Affara, Nabeel A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tirosvoutis, Konstantinos N.</au><au>Divane, Aspasia</au><au>Jones, Michael</au><au>Affara, Nabeel A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a Novel Zinc Finger Gene (ZNF165) Mapping to 6p21 That Is Expressed Specifically in Testis</atitle><jtitle>Genomics</jtitle><addtitle>Genomics</addtitle><date>1995-08-10</date><risdate>1995</risdate><volume>28</volume><issue>3</issue><spage>485</spage><epage>490</epage><pages>485-490</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Clone ZF388 was identified during the course of expressed sequence tag analysis of human adult testis cDNAs as being a member of the C2H2 zinc finger gene family. Northern blot analysis of a range of 16 different human tissues has shown that clone ZF388 detects a single 2.2-kb transcript and that the expression of this mRNA species is strictly specific to the testis. Sequence analysis of ZF388 and other clones isolated by rescreening a testis cDNA library has defined a transcript with an extensive N-terminal acidic domain containing 17% aspartic and glutamic acid residues and a C-terminal domain composed of five zinc finger motifs linked through the highly conserved sequence TGE-KPYE. This gene has been designated ZNF165. Analysis of somatic cell hybrids containing single human chromosomes shows that ZNF165 maps to chromosome 6. Fluorescence
in situ hybridization of cDNA insert probes to male metaphase spreads shows that the ZNF165 transcript maps to 6p21 close to the human MHC region. Human 6p21 is homologous with the distal inversion of the mouse t-complex, which has been shown to contain a cluster of genes expressed specifically in the testis.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7490084</pmid><doi>10.1006/geno.1995.1178</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Animals Base Sequence Biological and medical sciences BIOLOGY AND MEDICINE, BASIC STUDIES Chromosome Mapping Chromosomes, Human, Pair 6 Cloning, Molecular Cricetinae DNA - analysis DNA HYBRIDIZATION DNA Primers DNA SEQUENCING DNA-Binding Proteins - genetics DNA-CLONING FLUORESCENCE Fundamental and applied biological sciences. Psychology Gene Expression Regulation GENE REGULATION GENES Genes. Genome GENETIC MAPPING HISTOCOMPATIBILITY COMPLEX HUMAN CHROMOSOME 6 Humans HYBRIDIZATION Male Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data PROBES PROTEINS SOMATIC CELLS TESTES TRANSCRIPTION Zinc Fingers - genetics |
| title | Characterization of a Novel Zinc Finger Gene (ZNF165) Mapping to 6p21 That Is Expressed Specifically in Testis |
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