Automated linkage analysis in psychiatric disorders
A genome‐wide search for linkage of micro‐satellite markers to chromosomal loci containing genes responsible for the major psychoses is a laborious task which can be carried out with greater speed and economy by introducing automation to several steps in the procedure. We describe the use of the Aut...
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| Veröffentlicht in: | American Journal of Medical Genetics 1995-06, Vol.60 (3), p.192-198 |
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| creator | He, L. Mansfield, D. C. Brown, A. F. Green, D. K. Morris, S. W. St. Clair, D. M. Muir, W. J. Maclean, A. Wright, A. F. Blackwood, D. H. R. |
| description | A genome‐wide search for linkage of micro‐satellite markers to chromosomal loci containing genes responsible for the major psychoses is a laborious task which can be carried out with greater speed and economy by introducing automation to several steps in the procedure.
We describe the use of the Automated Linkage Preprocessor (ALP) program for the computer analysis of the waveform generated by fluorescein‐labelled markers after electrophoretic separation. (To obtain a copy send a request to A.F. Brown at the below MRC address or use Anonymous FTP to ftp.hgu.mrc.ac.uk. Software is in directory pub/ALP.) The program runs on a PC in the Microsoft Windows environment, and is used in conjunction with an automated laser fluorescence (ALF) sequencer (Pharmacia) and its Fragment Manager™ software to detect and size the PCR products, filter out peaks of fluorescence due to nonallele fragments, and generate genotypes in a format suitable for direct input to standard linkage analysis programs.
The method should offer the advantages of speed, accuracy, and reduced cost. Its use in linkage studies in a large family with manic‐depressive illness is discussed. © 1995 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/ajmg.1320600305 |
| format | Article |
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We describe the use of the Automated Linkage Preprocessor (ALP) program for the computer analysis of the waveform generated by fluorescein‐labelled markers after electrophoretic separation. (To obtain a copy send a request to A.F. Brown at the below MRC address or use Anonymous FTP to ftp.hgu.mrc.ac.uk. Software is in directory pub/ALP.) The program runs on a PC in the Microsoft Windows environment, and is used in conjunction with an automated laser fluorescence (ALF) sequencer (Pharmacia) and its Fragment Manager™ software to detect and size the PCR products, filter out peaks of fluorescence due to nonallele fragments, and generate genotypes in a format suitable for direct input to standard linkage analysis programs.
The method should offer the advantages of speed, accuracy, and reduced cost. Its use in linkage studies in a large family with manic‐depressive illness is discussed. © 1995 Wiley‐Liss, Inc.</description><identifier>ISSN: 0148-7299</identifier><identifier>EISSN: 1096-8628</identifier><identifier>DOI: 10.1002/ajmg.1320600305</identifier><identifier>PMID: 7573170</identifier><identifier>CODEN: AJMGDA</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>ACCURACY ; Adult and adolescent clinical studies ; AUTOMATION ; Biological and medical sciences ; BIOLOGICAL MARKERS ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Chromosome Mapping ; COMPUTER CODES ; COST ; DNA - analysis ; DNA SEQUENCERS ; DNA SEQUENCING ; EFFICIENCY ; ELECTROPHORESIS ; gene mapping ; GENES ; Genetic Linkage ; GENETIC MAPPING ; GENOTYPE ; HEREDITARY DISEASES ; HUMAN CHROMOSOMES ; Humans ; INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS ; linkage ; linkage analysis ; MAN ; MATHEMATICS, COMPUTERS, INFORMATION SCIENCE, MANAGEMENT, LAW, MISCELLANEOUS ; Medical sciences ; MENTAL DISORDERS ; POLYMERASE CHAIN REACTION ; psychiatric disease ; Psychology. Psychoanalysis. Psychiatry ; Psychopathology. Psychiatry ; Psychotic Disorders - genetics ; Software ; Somatoform disorders. Psychosomatics</subject><ispartof>American Journal of Medical Genetics, 1995-06, Vol.60 (3), p.192-198</ispartof><rights>Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4685-947e4d021d5bef5f3755b70a21d21380861c6674858ab1faac6b0bc818ab7cdc3</citedby><cites>FETCH-LOGICAL-c4685-947e4d021d5bef5f3755b70a21d21380861c6674858ab1faac6b0bc818ab7cdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3574237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7573170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/443840$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>He, L.</creatorcontrib><creatorcontrib>Mansfield, D. C.</creatorcontrib><creatorcontrib>Brown, A. F.</creatorcontrib><creatorcontrib>Green, D. K.</creatorcontrib><creatorcontrib>Morris, S. W.</creatorcontrib><creatorcontrib>St. Clair, D. M.</creatorcontrib><creatorcontrib>Muir, W. J.</creatorcontrib><creatorcontrib>Maclean, A.</creatorcontrib><creatorcontrib>Wright, A. F.</creatorcontrib><creatorcontrib>Blackwood, D. H. R.</creatorcontrib><title>Automated linkage analysis in psychiatric disorders</title><title>American Journal of Medical Genetics</title><addtitle>Am. J. Med. Genet</addtitle><description>A genome‐wide search for linkage of micro‐satellite markers to chromosomal loci containing genes responsible for the major psychoses is a laborious task which can be carried out with greater speed and economy by introducing automation to several steps in the procedure.
We describe the use of the Automated Linkage Preprocessor (ALP) program for the computer analysis of the waveform generated by fluorescein‐labelled markers after electrophoretic separation. (To obtain a copy send a request to A.F. Brown at the below MRC address or use Anonymous FTP to ftp.hgu.mrc.ac.uk. Software is in directory pub/ALP.) The program runs on a PC in the Microsoft Windows environment, and is used in conjunction with an automated laser fluorescence (ALF) sequencer (Pharmacia) and its Fragment Manager™ software to detect and size the PCR products, filter out peaks of fluorescence due to nonallele fragments, and generate genotypes in a format suitable for direct input to standard linkage analysis programs.
The method should offer the advantages of speed, accuracy, and reduced cost. Its use in linkage studies in a large family with manic‐depressive illness is discussed. © 1995 Wiley‐Liss, Inc.</description><subject>ACCURACY</subject><subject>Adult and adolescent clinical studies</subject><subject>AUTOMATION</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MARKERS</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Chromosome Mapping</subject><subject>COMPUTER CODES</subject><subject>COST</subject><subject>DNA - analysis</subject><subject>DNA SEQUENCERS</subject><subject>DNA SEQUENCING</subject><subject>EFFICIENCY</subject><subject>ELECTROPHORESIS</subject><subject>gene mapping</subject><subject>GENES</subject><subject>Genetic Linkage</subject><subject>GENETIC MAPPING</subject><subject>GENOTYPE</subject><subject>HEREDITARY DISEASES</subject><subject>HUMAN CHROMOSOMES</subject><subject>Humans</subject><subject>INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS</subject><subject>linkage</subject><subject>linkage analysis</subject><subject>MAN</subject><subject>MATHEMATICS, COMPUTERS, INFORMATION SCIENCE, MANAGEMENT, LAW, MISCELLANEOUS</subject><subject>Medical sciences</subject><subject>MENTAL DISORDERS</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>psychiatric disease</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopathology. Psychiatry</subject><subject>Psychotic Disorders - genetics</subject><subject>Software</subject><subject>Somatoform disorders. Psychosomatics</subject><issn>0148-7299</issn><issn>1096-8628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtvEzEQhy1EVdLCmRPSIiFu247fjjhFEaQvHhIgjpbX623d7iP1bFTy3-Nqo6CeerI88_1mRh8hbymcUAB26m676xPKGSgADvIFmVGYq9IoZl6SGVBhSs3m81fkCPEWgOYCOySHWmpONcwIX2zGoXNjqIs29nfuOhSud-0WIxaxL9a49TfRjSn6oo44pDokfE0OGtdieLN7j8nvL59_Lc_Kq--r8-XiqvRCGVnOhQ6iBkZrWYVGNlxLWWlwucAoN2AU9UppYaRxFW2c86qCyhuav9rXnh-T99PcAcdo0ccx-Bs_9H3woxWCGwGZ-Tgx6zTcbwKOtovoQ9u6PgwbtFpLY6iQz4I0n8wEiAyeTqBPA2IKjV2n2Lm0tRTso3P76Nz-d54T73ajN1UX6j2_k5z7H3Z9h961TXK9j7jHuNSCcZ2xTxP2ENuwfW6rXVx8XT05opzSEcfwd5926c4qndXbP99WdvXzgsPZj0u75P8A15Cokw</recordid><startdate>19950619</startdate><enddate>19950619</enddate><creator>He, L.</creator><creator>Mansfield, D. C.</creator><creator>Brown, A. F.</creator><creator>Green, D. K.</creator><creator>Morris, S. W.</creator><creator>St. Clair, D. M.</creator><creator>Muir, W. J.</creator><creator>Maclean, A.</creator><creator>Wright, A. F.</creator><creator>Blackwood, D. H. R.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19950619</creationdate><title>Automated linkage analysis in psychiatric disorders</title><author>He, L. ; Mansfield, D. C. ; Brown, A. F. ; Green, D. K. ; Morris, S. W. ; St. Clair, D. M. ; Muir, W. J. ; Maclean, A. ; Wright, A. F. ; Blackwood, D. H. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4685-947e4d021d5bef5f3755b70a21d21380861c6674858ab1faac6b0bc818ab7cdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ACCURACY</topic><topic>Adult and adolescent clinical studies</topic><topic>AUTOMATION</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MARKERS</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Chromosome Mapping</topic><topic>COMPUTER CODES</topic><topic>COST</topic><topic>DNA - analysis</topic><topic>DNA SEQUENCERS</topic><topic>DNA SEQUENCING</topic><topic>EFFICIENCY</topic><topic>ELECTROPHORESIS</topic><topic>gene mapping</topic><topic>GENES</topic><topic>Genetic Linkage</topic><topic>GENETIC MAPPING</topic><topic>GENOTYPE</topic><topic>HEREDITARY DISEASES</topic><topic>HUMAN CHROMOSOMES</topic><topic>Humans</topic><topic>INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS</topic><topic>linkage</topic><topic>linkage analysis</topic><topic>MAN</topic><topic>MATHEMATICS, COMPUTERS, INFORMATION SCIENCE, MANAGEMENT, LAW, MISCELLANEOUS</topic><topic>Medical sciences</topic><topic>MENTAL DISORDERS</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>psychiatric disease</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopathology. Psychiatry</topic><topic>Psychotic Disorders - genetics</topic><topic>Software</topic><topic>Somatoform disorders. Psychosomatics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, L.</creatorcontrib><creatorcontrib>Mansfield, D. C.</creatorcontrib><creatorcontrib>Brown, A. F.</creatorcontrib><creatorcontrib>Green, D. K.</creatorcontrib><creatorcontrib>Morris, S. W.</creatorcontrib><creatorcontrib>St. Clair, D. M.</creatorcontrib><creatorcontrib>Muir, W. J.</creatorcontrib><creatorcontrib>Maclean, A.</creatorcontrib><creatorcontrib>Wright, A. F.</creatorcontrib><creatorcontrib>Blackwood, D. H. 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R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automated linkage analysis in psychiatric disorders</atitle><jtitle>American Journal of Medical Genetics</jtitle><addtitle>Am. J. Med. Genet</addtitle><date>1995-06-19</date><risdate>1995</risdate><volume>60</volume><issue>3</issue><spage>192</spage><epage>198</epage><pages>192-198</pages><issn>0148-7299</issn><eissn>1096-8628</eissn><coden>AJMGDA</coden><abstract>A genome‐wide search for linkage of micro‐satellite markers to chromosomal loci containing genes responsible for the major psychoses is a laborious task which can be carried out with greater speed and economy by introducing automation to several steps in the procedure.
We describe the use of the Automated Linkage Preprocessor (ALP) program for the computer analysis of the waveform generated by fluorescein‐labelled markers after electrophoretic separation. (To obtain a copy send a request to A.F. Brown at the below MRC address or use Anonymous FTP to ftp.hgu.mrc.ac.uk. Software is in directory pub/ALP.) The program runs on a PC in the Microsoft Windows environment, and is used in conjunction with an automated laser fluorescence (ALF) sequencer (Pharmacia) and its Fragment Manager™ software to detect and size the PCR products, filter out peaks of fluorescence due to nonallele fragments, and generate genotypes in a format suitable for direct input to standard linkage analysis programs.
The method should offer the advantages of speed, accuracy, and reduced cost. Its use in linkage studies in a large family with manic‐depressive illness is discussed. © 1995 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7573170</pmid><doi>10.1002/ajmg.1320600305</doi><tpages>7</tpages></addata></record> |
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| subjects | ACCURACY Adult and adolescent clinical studies AUTOMATION Biological and medical sciences BIOLOGICAL MARKERS BIOLOGY AND MEDICINE, BASIC STUDIES Chromosome Mapping COMPUTER CODES COST DNA - analysis DNA SEQUENCERS DNA SEQUENCING EFFICIENCY ELECTROPHORESIS gene mapping GENES Genetic Linkage GENETIC MAPPING GENOTYPE HEREDITARY DISEASES HUMAN CHROMOSOMES Humans INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS linkage linkage analysis MAN MATHEMATICS, COMPUTERS, INFORMATION SCIENCE, MANAGEMENT, LAW, MISCELLANEOUS Medical sciences MENTAL DISORDERS POLYMERASE CHAIN REACTION psychiatric disease Psychology. Psychoanalysis. Psychiatry Psychopathology. Psychiatry Psychotic Disorders - genetics Software Somatoform disorders. Psychosomatics |
| title | Automated linkage analysis in psychiatric disorders |
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