The bldA-encoded tRNA is poorly expressed in the bldi mutant of Streptomyces coelicolor A3(2)

Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 Author for correspondence: Brenda K. Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca ABSTRACT The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is...

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Veröffentlicht in:Microbiology (Society for General Microbiology) 1995-08, Vol.141 (8), p.1921-1926
Hauptverfasser: Leskiw, Brenda K, Mah, Richard
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container_end_page 1926
container_issue 8
container_start_page 1921
container_title Microbiology (Society for General Microbiology)
container_volume 141
creator Leskiw, Brenda K
Mah, Richard
description Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 Author for correspondence: Brenda K. Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca ABSTRACT The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldii mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldi mutant, as was the mature, 87 nucleotide, form of the bldA -encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldi mutant, suggesting that maximal bldA expression may require a bldii -dependent promoter. Keywords: Streptomyes coelicolor, bldA, bldl, antibiotic production, morphological differentiation
doi_str_mv 10.1099/13500872-141-8-1921
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Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca ABSTRACT The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldii mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldi mutant, as was the mature, 87 nucleotide, form of the bldA -encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldi mutant, suggesting that maximal bldA expression may require a bldii -dependent promoter. 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Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca ABSTRACT The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldii mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldi mutant, as was the mature, 87 nucleotide, form of the bldA -encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldi mutant, suggesting that maximal bldA expression may require a bldii -dependent promoter. 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Psychology</subject><subject>Gene Dosage</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Reporter</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Biosynthesis</subject><subject>RNA, Transfer, Leu - biosynthesis</subject><subject>RNA, Transfer, Leu - chemistry</subject><subject>RNA, Transfer, Leu - genetics</subject><subject>Spores</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - growth &amp; development</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces coelicolor</subject><subject>Transformation, Bacterial</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1rFDEUhoMotVZ_gQi5kGIvojn5mszlUvyC0oLWSwmZ7JluZGYyJrPU_fdm2bV416sceJ9z8vIQ8hr4e-Bt-wGk5tw2goECZhm0Ap6QU1BGM8Etf1rnSrA98py8KOUX5zXkcEJOGq2Ba3VKft5ukHbDesVwCmmNa7p8u17RWOicUh52FP_MGUupQZzocoAjHbeLnxaaevp9yTgvadwFLDQkHGJIQ8p0Jd-Ji5fkWe-Hgq-O7xn58enj7eUXdnXz-evl6ooFYQ0waZrQ2tY3aKBFK7mySjcSdNBScNmazoDqtfIdeOCd4cIKb4LqutADWi3PyPnh7pzT7y2WxY2xBBwGP2HaFtc02kow7aMgaKOqF1tBeQBDTqVk7N2c4-jzzgF3e_vun31X7Tvr9vbr1pvj-W034vph56i75m-PuS_BD332U4jlAZNGaKX2LS8O2Cbebe5jRneH0xhrlS6m2jj89-VfBD2XpA</recordid><startdate>199508</startdate><enddate>199508</enddate><creator>Leskiw, Brenda K</creator><creator>Mah, Richard</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199508</creationdate><title>The bldA-encoded tRNA is poorly expressed in the bldi mutant of Streptomyces coelicolor A3(2)</title><author>Leskiw, Brenda K ; Mah, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2861-367c989a7e619e83048457315c5320396b614f54ab1a10b60282a6c4bbcf1e853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Blotting, Northern</topic><topic>Fundamental and applied biological sciences. 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Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca ABSTRACT The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldii mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldi mutant, as was the mature, 87 nucleotide, form of the bldA -encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldi mutant, suggesting that maximal bldA expression may require a bldii -dependent promoter. Keywords: Streptomyes coelicolor, bldA, bldl, antibiotic production, morphological differentiation</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>7551054</pmid><doi>10.1099/13500872-141-8-1921</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; PubMed Central
subjects Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Blotting, Northern
Fundamental and applied biological sciences. Psychology
Gene Dosage
Gene Expression Regulation, Bacterial
Genes, Reporter
Genetics
Microbiology
Mission oriented research
Molecular Sequence Data
Mutation
Plasmids
Polymerase Chain Reaction
Protein Biosynthesis
RNA, Transfer, Leu - biosynthesis
RNA, Transfer, Leu - chemistry
RNA, Transfer, Leu - genetics
Spores
Streptomyces - genetics
Streptomyces - growth & development
Streptomyces - metabolism
Streptomyces coelicolor
Transformation, Bacterial
title The bldA-encoded tRNA is poorly expressed in the bldi mutant of Streptomyces coelicolor A3(2)
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