The bldA-encoded tRNA is poorly expressed in the bldi mutant of Streptomyces coelicolor A3(2)
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 Author for correspondence: Brenda K. Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca ABSTRACT The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is...
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Veröffentlicht in: | Microbiology (Society for General Microbiology) 1995-08, Vol.141 (8), p.1921-1926 |
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container_end_page | 1926 |
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container_issue | 8 |
container_start_page | 1921 |
container_title | Microbiology (Society for General Microbiology) |
container_volume | 141 |
creator | Leskiw, Brenda K Mah, Richard |
description | Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
Author for correspondence: Brenda K. Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca
ABSTRACT
The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldii mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldi mutant, as was the mature, 87 nucleotide, form of the bldA -encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldi mutant, suggesting that maximal bldA expression may require a bldii -dependent promoter.
Keywords: Streptomyes coelicolor, bldA, bldl, antibiotic production, morphological differentiation |
doi_str_mv | 10.1099/13500872-141-8-1921 |
format | Article |
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Author for correspondence: Brenda K. Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca
ABSTRACT
The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldii mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldi mutant, as was the mature, 87 nucleotide, form of the bldA -encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldi mutant, suggesting that maximal bldA expression may require a bldii -dependent promoter.
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Author for correspondence: Brenda K. Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca
ABSTRACT
The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldii mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldi mutant, as was the mature, 87 nucleotide, form of the bldA -encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldi mutant, suggesting that maximal bldA expression may require a bldii -dependent promoter.
Keywords: Streptomyes coelicolor, bldA, bldl, antibiotic production, morphological differentiation</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Blotting, Northern</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Dosage</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Reporter</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Biosynthesis</subject><subject>RNA, Transfer, Leu - biosynthesis</subject><subject>RNA, Transfer, Leu - chemistry</subject><subject>RNA, Transfer, Leu - genetics</subject><subject>Spores</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - growth & development</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces coelicolor</subject><subject>Transformation, Bacterial</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1rFDEUhoMotVZ_gQi5kGIvojn5mszlUvyC0oLWSwmZ7JluZGYyJrPU_fdm2bV416sceJ9z8vIQ8hr4e-Bt-wGk5tw2goECZhm0Ap6QU1BGM8Etf1rnSrA98py8KOUX5zXkcEJOGq2Ba3VKft5ukHbDesVwCmmNa7p8u17RWOicUh52FP_MGUupQZzocoAjHbeLnxaaevp9yTgvadwFLDQkHGJIQ8p0Jd-Ji5fkWe-Hgq-O7xn58enj7eUXdnXz-evl6ooFYQ0waZrQ2tY3aKBFK7mySjcSdNBScNmazoDqtfIdeOCd4cIKb4LqutADWi3PyPnh7pzT7y2WxY2xBBwGP2HaFtc02kow7aMgaKOqF1tBeQBDTqVk7N2c4-jzzgF3e_vun31X7Tvr9vbr1pvj-W034vph56i75m-PuS_BD332U4jlAZNGaKX2LS8O2Cbebe5jRneH0xhrlS6m2jj89-VfBD2XpA</recordid><startdate>199508</startdate><enddate>199508</enddate><creator>Leskiw, Brenda K</creator><creator>Mah, Richard</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199508</creationdate><title>The bldA-encoded tRNA is poorly expressed in the bldi mutant of Streptomyces coelicolor A3(2)</title><author>Leskiw, Brenda K ; Mah, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2861-367c989a7e619e83048457315c5320396b614f54ab1a10b60282a6c4bbcf1e853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Blotting, Northern</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Dosage</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Reporter</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Protein Biosynthesis</topic><topic>RNA, Transfer, Leu - biosynthesis</topic><topic>RNA, Transfer, Leu - chemistry</topic><topic>RNA, Transfer, Leu - genetics</topic><topic>Spores</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - growth & development</topic><topic>Streptomyces - metabolism</topic><topic>Streptomyces coelicolor</topic><topic>Transformation, Bacterial</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leskiw, Brenda K</creatorcontrib><creatorcontrib>Mah, Richard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leskiw, Brenda K</au><au>Mah, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The bldA-encoded tRNA is poorly expressed in the bldi mutant of Streptomyces coelicolor A3(2)</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology (Reading)</addtitle><date>1995-08</date><risdate>1995</risdate><volume>141</volume><issue>8</issue><spage>1921</spage><epage>1926</epage><pages>1921-1926</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
Author for correspondence: Brenda K. Leskiw. Tel: +1 403 492 1868. Fax: + 1 403 492 9234. e-mail: brenda_leskiw@biology.ualberta.ca
ABSTRACT
The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldii mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldi mutant, as was the mature, 87 nucleotide, form of the bldA -encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldi mutant, suggesting that maximal bldA expression may require a bldii -dependent promoter.
Keywords: Streptomyes coelicolor, bldA, bldl, antibiotic production, morphological differentiation</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>7551054</pmid><doi>10.1099/13500872-141-8-1921</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Base Sequence Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Blotting, Northern Fundamental and applied biological sciences. Psychology Gene Dosage Gene Expression Regulation, Bacterial Genes, Reporter Genetics Microbiology Mission oriented research Molecular Sequence Data Mutation Plasmids Polymerase Chain Reaction Protein Biosynthesis RNA, Transfer, Leu - biosynthesis RNA, Transfer, Leu - chemistry RNA, Transfer, Leu - genetics Spores Streptomyces - genetics Streptomyces - growth & development Streptomyces - metabolism Streptomyces coelicolor Transformation, Bacterial |
title | The bldA-encoded tRNA is poorly expressed in the bldi mutant of Streptomyces coelicolor A3(2) |
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