Formation of lipoxin A by granulocytes from eosinophilic donors
The formation of arachidonic acid-derived lipoxygenase products was examined with human granulocytes obtained from eosinophilic donors. These eosinophil-enriched leukocyte populations, challenged in vitro with the ionophore of divalent cations A23187, transformed both exogenous and endogenous source...
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| Veröffentlicht in: | FEBS letters 1987-06, Vol.217 (2), p.242-246 |
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| creator | Serhan, Charles N. Hirsch, Ulf Palmblad, Jan Samuelsson, Bengt |
| description | The formation of arachidonic acid-derived lipoxygenase products was examined with human granulocytes obtained from eosinophilic donors. These eosinophil-enriched leukocyte populations, challenged in vitro with the ionophore of divalent cations A23187, transformed both exogenous and endogenous sources of arachidonic acid to several lipoxygenase-derived products, including 5(
S),6(
R),15(
S)-trihydroxy-7,9, 13-
trans-11-
cis-eicosatetraenoic acid (lipoxin A). Lipoxin A was detected and characterized by high-pressure liquid chromatography (HPLC), ultraviolet absorbance, and gas-liquid chromatography-mass spectroscopy. Neither lipoxin B nor 6(
S)-LXA was consistently detected in extracts from these incubations. The amounts of lipoxin A formed were proportional to the percentage of eosinophils present in the suspension. The results indicate that granulocytes from eosinophilic donors can generate lipoxin A. |
| doi_str_mv | 10.1016/0014-5793(87)80671-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77582575</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0014579387806713</els_id><sourcerecordid>77582575</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4493-7ae3f89b1124b8f33431d452d0e7b4788e7dbaf80b2d09a72494ffe421eee39f3</originalsourceid><addsrcrecordid>eNqNkMFPwyAYxYnRzDn9DzTpwRg9VKHQARfNXDY1WeJFz4TSD8W0ZcKm7r-33ZYdjSfCe-97H_wQOiX4mmAyvMGYsDTnkl4KfiXwkJOU7qE-EZymlA3FPurvIofoKMYP3N4FkT3UowRLybI-upv6UOuF803ibVK5uf9xTTJKilXyFnSzrLxZLSAmNvg6AR9d4-fvrnImKX3jQzxGB1ZXEU625wC9Ticv48d09vzwNB7NUsOYpCnXQK2QBSEZK4SllFFSsjwrMfCCcSGAl4W2AhetJDXPmGTWAssIAFBp6QBdbHrnwX8uIS5U7aKBqtIN-GVUnOciy3neBtkmaIKPMYBV8-BqHVaKYNVxUx0U1UFRgqs1N0XbsbNt_7KoodwNbUG1_vnW19HoyrZsjIu7GM_zjNNhG5tuYt-ugtW_Vqvp5D7rjE4XfK1277ndFEEL9ctBUNE4aAyULoBZqNK7vz_0C9QgnAo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77582575</pqid></control><display><type>article</type><title>Formation of lipoxin A by granulocytes from eosinophilic donors</title><source>MEDLINE</source><source>ScienceDirect Freedom Collection (Elsevier)</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Serhan, Charles N. ; Hirsch, Ulf ; Palmblad, Jan ; Samuelsson, Bengt</creator><creatorcontrib>Serhan, Charles N. ; Hirsch, Ulf ; Palmblad, Jan ; Samuelsson, Bengt</creatorcontrib><description>The formation of arachidonic acid-derived lipoxygenase products was examined with human granulocytes obtained from eosinophilic donors. These eosinophil-enriched leukocyte populations, challenged in vitro with the ionophore of divalent cations A23187, transformed both exogenous and endogenous sources of arachidonic acid to several lipoxygenase-derived products, including 5(
S),6(
R),15(
S)-trihydroxy-7,9, 13-
trans-11-
cis-eicosatetraenoic acid (lipoxin A). Lipoxin A was detected and characterized by high-pressure liquid chromatography (HPLC), ultraviolet absorbance, and gas-liquid chromatography-mass spectroscopy. Neither lipoxin B nor 6(
S)-LXA was consistently detected in extracts from these incubations. The amounts of lipoxin A formed were proportional to the percentage of eosinophils present in the suspension. The results indicate that granulocytes from eosinophilic donors can generate lipoxin A.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(87)80671-3</identifier><identifier>PMID: 3109942</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>15 -HETE, 15(S)-hydroxy-5,8,11-cis-13-trans -eicosatetraenoic acid ; 5,15 -DHETE, 5(S),15(S)-dihydroxy-6,13-trans-8,11-cis -eicosatetraenoic acid ; 6(S)-LXA, 5(S),6(S),15(S)-trihydroxy-7,9,13-trans-11 -cis-eicosatetraenoic acid ; Analytical, structural and metabolic biochemistry ; Arachidonate 5-Lipoxygenase - metabolism ; Arachidonic Acid ; Arachidonic Acids - metabolism ; Biological and medical sciences ; Calcimycin - pharmacology ; Eosinophil ; Eosinophilia - pathology ; Fundamental and applied biological sciences. Psychology ; GC, gas-liquid chromatography ; Granulocytes - drug effects ; Granulocytes - metabolism ; Granulocytes - pathology ; HES, hypereosinophilic syndrome ; Human ; Humans ; Hydroxyeicosatetraenoic Acids - biosynthesis ; Leukocyte ; Lipids ; lipoxin A (LXA), 5(S),6(R),15(S) -trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid ; lipoxin B (LXB), 5(S),14(R),15(S) -trihydroxy-6,10,12-trans-8-cis-eicosatetraenoic acid ; Lipoxins ; Lipoxygenase product ; LX, lipoxin ; Me3Si, trimethylsilyl ; MS, mass spectrometry ; Other biological molecules ; RP-HPLC, reverse-phase high-pressure liquid chromatography ; SRS-A - biosynthesis</subject><ispartof>FEBS letters, 1987-06, Vol.217 (2), p.242-246</ispartof><rights>1987</rights><rights>FEBS Letters 217 (1987) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4493-7ae3f89b1124b8f33431d452d0e7b4788e7dbaf80b2d09a72494ffe421eee39f3</citedby><cites>FETCH-LOGICAL-c4493-7ae3f89b1124b8f33431d452d0e7b4788e7dbaf80b2d09a72494ffe421eee39f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(87)80671-3$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7552736$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3109942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Serhan, Charles N.</creatorcontrib><creatorcontrib>Hirsch, Ulf</creatorcontrib><creatorcontrib>Palmblad, Jan</creatorcontrib><creatorcontrib>Samuelsson, Bengt</creatorcontrib><title>Formation of lipoxin A by granulocytes from eosinophilic donors</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The formation of arachidonic acid-derived lipoxygenase products was examined with human granulocytes obtained from eosinophilic donors. These eosinophil-enriched leukocyte populations, challenged in vitro with the ionophore of divalent cations A23187, transformed both exogenous and endogenous sources of arachidonic acid to several lipoxygenase-derived products, including 5(
S),6(
R),15(
S)-trihydroxy-7,9, 13-
trans-11-
cis-eicosatetraenoic acid (lipoxin A). Lipoxin A was detected and characterized by high-pressure liquid chromatography (HPLC), ultraviolet absorbance, and gas-liquid chromatography-mass spectroscopy. Neither lipoxin B nor 6(
S)-LXA was consistently detected in extracts from these incubations. The amounts of lipoxin A formed were proportional to the percentage of eosinophils present in the suspension. The results indicate that granulocytes from eosinophilic donors can generate lipoxin A.</description><subject>15 -HETE, 15(S)-hydroxy-5,8,11-cis-13-trans -eicosatetraenoic acid</subject><subject>5,15 -DHETE, 5(S),15(S)-dihydroxy-6,13-trans-8,11-cis -eicosatetraenoic acid</subject><subject>6(S)-LXA, 5(S),6(S),15(S)-trihydroxy-7,9,13-trans-11 -cis-eicosatetraenoic acid</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Arachidonate 5-Lipoxygenase - metabolism</subject><subject>Arachidonic Acid</subject><subject>Arachidonic Acids - metabolism</subject><subject>Biological and medical sciences</subject><subject>Calcimycin - pharmacology</subject><subject>Eosinophil</subject><subject>Eosinophilia - pathology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GC, gas-liquid chromatography</subject><subject>Granulocytes - drug effects</subject><subject>Granulocytes - metabolism</subject><subject>Granulocytes - pathology</subject><subject>HES, hypereosinophilic syndrome</subject><subject>Human</subject><subject>Humans</subject><subject>Hydroxyeicosatetraenoic Acids - biosynthesis</subject><subject>Leukocyte</subject><subject>Lipids</subject><subject>lipoxin A (LXA), 5(S),6(R),15(S) -trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid</subject><subject>lipoxin B (LXB), 5(S),14(R),15(S) -trihydroxy-6,10,12-trans-8-cis-eicosatetraenoic acid</subject><subject>Lipoxins</subject><subject>Lipoxygenase product</subject><subject>LX, lipoxin</subject><subject>Me3Si, trimethylsilyl</subject><subject>MS, mass spectrometry</subject><subject>Other biological molecules</subject><subject>RP-HPLC, reverse-phase high-pressure liquid chromatography</subject><subject>SRS-A - biosynthesis</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMFPwyAYxYnRzDn9DzTpwRg9VKHQARfNXDY1WeJFz4TSD8W0ZcKm7r-33ZYdjSfCe-97H_wQOiX4mmAyvMGYsDTnkl4KfiXwkJOU7qE-EZymlA3FPurvIofoKMYP3N4FkT3UowRLybI-upv6UOuF803ibVK5uf9xTTJKilXyFnSzrLxZLSAmNvg6AR9d4-fvrnImKX3jQzxGB1ZXEU625wC9Ticv48d09vzwNB7NUsOYpCnXQK2QBSEZK4SllFFSsjwrMfCCcSGAl4W2AhetJDXPmGTWAssIAFBp6QBdbHrnwX8uIS5U7aKBqtIN-GVUnOciy3neBtkmaIKPMYBV8-BqHVaKYNVxUx0U1UFRgqs1N0XbsbNt_7KoodwNbUG1_vnW19HoyrZsjIu7GM_zjNNhG5tuYt-ugtW_Vqvp5D7rjE4XfK1277ndFEEL9ctBUNE4aAyULoBZqNK7vz_0C9QgnAo</recordid><startdate>19870615</startdate><enddate>19870615</enddate><creator>Serhan, Charles N.</creator><creator>Hirsch, Ulf</creator><creator>Palmblad, Jan</creator><creator>Samuelsson, Bengt</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870615</creationdate><title>Formation of lipoxin A by granulocytes from eosinophilic donors</title><author>Serhan, Charles N. ; Hirsch, Ulf ; Palmblad, Jan ; Samuelsson, Bengt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4493-7ae3f89b1124b8f33431d452d0e7b4788e7dbaf80b2d09a72494ffe421eee39f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>15 -HETE, 15(S)-hydroxy-5,8,11-cis-13-trans -eicosatetraenoic acid</topic><topic>5,15 -DHETE, 5(S),15(S)-dihydroxy-6,13-trans-8,11-cis -eicosatetraenoic acid</topic><topic>6(S)-LXA, 5(S),6(S),15(S)-trihydroxy-7,9,13-trans-11 -cis-eicosatetraenoic acid</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Arachidonate 5-Lipoxygenase - metabolism</topic><topic>Arachidonic Acid</topic><topic>Arachidonic Acids - metabolism</topic><topic>Biological and medical sciences</topic><topic>Calcimycin - pharmacology</topic><topic>Eosinophil</topic><topic>Eosinophilia - pathology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GC, gas-liquid chromatography</topic><topic>Granulocytes - drug effects</topic><topic>Granulocytes - metabolism</topic><topic>Granulocytes - pathology</topic><topic>HES, hypereosinophilic syndrome</topic><topic>Human</topic><topic>Humans</topic><topic>Hydroxyeicosatetraenoic Acids - biosynthesis</topic><topic>Leukocyte</topic><topic>Lipids</topic><topic>lipoxin A (LXA), 5(S),6(R),15(S) -trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid</topic><topic>lipoxin B (LXB), 5(S),14(R),15(S) -trihydroxy-6,10,12-trans-8-cis-eicosatetraenoic acid</topic><topic>Lipoxins</topic><topic>Lipoxygenase product</topic><topic>LX, lipoxin</topic><topic>Me3Si, trimethylsilyl</topic><topic>MS, mass spectrometry</topic><topic>Other biological molecules</topic><topic>RP-HPLC, reverse-phase high-pressure liquid chromatography</topic><topic>SRS-A - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Serhan, Charles N.</creatorcontrib><creatorcontrib>Hirsch, Ulf</creatorcontrib><creatorcontrib>Palmblad, Jan</creatorcontrib><creatorcontrib>Samuelsson, Bengt</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Serhan, Charles N.</au><au>Hirsch, Ulf</au><au>Palmblad, Jan</au><au>Samuelsson, Bengt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formation of lipoxin A by granulocytes from eosinophilic donors</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1987-06-15</date><risdate>1987</risdate><volume>217</volume><issue>2</issue><spage>242</spage><epage>246</epage><pages>242-246</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The formation of arachidonic acid-derived lipoxygenase products was examined with human granulocytes obtained from eosinophilic donors. These eosinophil-enriched leukocyte populations, challenged in vitro with the ionophore of divalent cations A23187, transformed both exogenous and endogenous sources of arachidonic acid to several lipoxygenase-derived products, including 5(
S),6(
R),15(
S)-trihydroxy-7,9, 13-
trans-11-
cis-eicosatetraenoic acid (lipoxin A). Lipoxin A was detected and characterized by high-pressure liquid chromatography (HPLC), ultraviolet absorbance, and gas-liquid chromatography-mass spectroscopy. Neither lipoxin B nor 6(
S)-LXA was consistently detected in extracts from these incubations. The amounts of lipoxin A formed were proportional to the percentage of eosinophils present in the suspension. The results indicate that granulocytes from eosinophilic donors can generate lipoxin A.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3109942</pmid><doi>10.1016/0014-5793(87)80671-3</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1987-06, Vol.217 (2), p.242-246 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77582575 |
| source | MEDLINE; ScienceDirect Freedom Collection (Elsevier); Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | 15 -HETE, 15(S)-hydroxy-5,8,11-cis-13-trans -eicosatetraenoic acid 5,15 -DHETE, 5(S),15(S)-dihydroxy-6,13-trans-8,11-cis -eicosatetraenoic acid 6(S)-LXA, 5(S),6(S),15(S)-trihydroxy-7,9,13-trans-11 -cis-eicosatetraenoic acid Analytical, structural and metabolic biochemistry Arachidonate 5-Lipoxygenase - metabolism Arachidonic Acid Arachidonic Acids - metabolism Biological and medical sciences Calcimycin - pharmacology Eosinophil Eosinophilia - pathology Fundamental and applied biological sciences. Psychology GC, gas-liquid chromatography Granulocytes - drug effects Granulocytes - metabolism Granulocytes - pathology HES, hypereosinophilic syndrome Human Humans Hydroxyeicosatetraenoic Acids - biosynthesis Leukocyte Lipids lipoxin A (LXA), 5(S),6(R),15(S) -trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid lipoxin B (LXB), 5(S),14(R),15(S) -trihydroxy-6,10,12-trans-8-cis-eicosatetraenoic acid Lipoxins Lipoxygenase product LX, lipoxin Me3Si, trimethylsilyl MS, mass spectrometry Other biological molecules RP-HPLC, reverse-phase high-pressure liquid chromatography SRS-A - biosynthesis |
| title | Formation of lipoxin A by granulocytes from eosinophilic donors |
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