A universal approach to the expression of human and rabbit cytochrome P450s of the 2C subfamily in Escherichia coli

Human cytochrome P450s 2C8, 2C9, 2C18, and 2C19 and rabbit cytochrome P450s 2C1, 2C2, 2C4, 2C5, and 2C16 were expressed from their respective cDNAs in Escherichia coli as chimeric enzymes in which a portion of the N-terminal membrane anchor sequence was replaced with a modified sequence derived from...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1995-10, Vol.323 (1), p.87-96
Hauptverfasser:	Richardson, T H, Jung, F, Griffin, K J, Wester, M, Raucy, J L, Kemper, B, Bornheim, L M, Hassett, C, Omiecinski, C J, Johnson, E F
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Animals Aryl Hydrocarbon Hydroxylases Base Sequence Cloning, Molecular Cytochrome P-450 Enzyme System - biosynthesis Cytochrome P-450 Enzyme System - genetics DNA, Complementary - genetics DNA, Complementary - isolation & purification Escherichia coli - genetics Genetic Techniques Humans Molecular Sequence Data Rabbits Recombinant Fusion Proteins - genetics Steroid 16-alpha-Hydroxylase Steroid Hydroxylases - biosynthesis Steroid Hydroxylases - genetics
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