Improved flow cytometry of cellular DNA and RNA by on‐line reagent addition
Acridine orange (AO) staining enables flow cytometric measurement of cellular DNA and RNA. With the conventional procedure, red and green fluorescence intensities alter considerably in time, affecting the reliability and reproducibility of the results. A standardized simple technique of AO staining...
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| Veröffentlicht in: | Cytometry (New York, N.Y.) N.Y.), 1987-05, Vol.8 (3), p.335-338 |
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| container_title | Cytometry (New York, N.Y.) |
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| creator | Pennings, Arie Speth, Paul Wessels, Hans Haanen, Clemens |
| description | Acridine orange (AO) staining enables flow cytometric measurement of cellular DNA and RNA. With the conventional procedure, red and green fluorescence intensities alter considerably in time, affecting the reliability and reproducibility of the results. A standardized simple technique of AO staining is described that keeps the time between addition of detergent and AO and the subsequent measurement constant. Measurements by means of this on‐line method appear to be much more reproducible in comparison to those obtained using the conventional procedure. |
| doi_str_mv | 10.1002/cyto.990080315 |
| format | Article |
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With the conventional procedure, red and green fluorescence intensities alter considerably in time, affecting the reliability and reproducibility of the results. A standardized simple technique of AO staining is described that keeps the time between addition of detergent and AO and the subsequent measurement constant. Measurements by means of this on‐line method appear to be much more reproducible in comparison to those obtained using the conventional procedure.</description><identifier>ISSN: 0196-4763</identifier><identifier>EISSN: 1097-0320</identifier><identifier>DOI: 10.1002/cyto.990080315</identifier><identifier>PMID: 2439265</identifier><identifier>CODEN: CYTODQ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Acridine orange ; Biological and medical sciences ; Cell Line ; Diverse techniques ; DNA - analysis ; DNA, Neoplasm - analysis ; Flow Cytometry - instrumentation ; Flow Cytometry - methods ; Fundamental and applied biological sciences. 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With the conventional procedure, red and green fluorescence intensities alter considerably in time, affecting the reliability and reproducibility of the results. A standardized simple technique of AO staining is described that keeps the time between addition of detergent and AO and the subsequent measurement constant. Measurements by means of this on‐line method appear to be much more reproducible in comparison to those obtained using the conventional procedure.</description><subject>Acridine orange</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Diverse techniques</subject><subject>DNA - analysis</subject><subject>DNA, Neoplasm - analysis</subject><subject>Flow Cytometry - instrumentation</subject><subject>Flow Cytometry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Indicators and Reagents</subject><subject>Leukemia, Lymphoid</subject><subject>Molecular and cellular biology</subject><subject>RNA - analysis</subject><subject>RNA, Neoplasm - analysis</subject><subject>Staining and Labeling</subject><subject>standardized staining methods</subject><issn>0196-4763</issn><issn>1097-0320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtOxDAQRS0EgmWhpUNygeiyjOPYjku0PCUeEoKCKvJjgoLyADsL2o5P4Bv5ErLa1VJSTXHP3LlzCTlgMGEA6Ymb991Ea4AcOBMbZMRAqwR4CptkBEzLJFOS75DdGF8BQMuMb5PtNOM6lWJEbq-bt9B9oKdl3X3ShVuDfZjTrqQO63pWm0DP7k6paT19GKYdpPbn67uuWqQBzQu2PTXeV33VtXtkqzR1xP3VHJOni_PH6VVyc395PT29SRxPM5F4LbgFYa3MpFfCp146jtwh5B59aVPhGFOpzYQCzCVDAZnhzMrcMm1R8DE5XvoO2d9nGPuiqeIirmmxm8VCKaFyMbw6JpMl6EIXY8CyeAtVY8K8YFAs-isWHxfr_oaFw5XzzDbo1_iqsEE_WukmOlOXwbSuimtMcaWk0gOml9hnVeP8n6PF9Pnx_i_CL_YaieQ</recordid><startdate>198705</startdate><enddate>198705</enddate><creator>Pennings, Arie</creator><creator>Speth, Paul</creator><creator>Wessels, Hans</creator><creator>Haanen, Clemens</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198705</creationdate><title>Improved flow cytometry of cellular DNA and RNA by on‐line reagent addition</title><author>Pennings, Arie ; Speth, Paul ; Wessels, Hans ; Haanen, Clemens</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3245-d953b05bb646d75d2d6c3e3ce08dedfb25c1172b4570e861e504a31b68b19be53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Acridine orange</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Diverse techniques</topic><topic>DNA - analysis</topic><topic>DNA, Neoplasm - analysis</topic><topic>Flow Cytometry - instrumentation</topic><topic>Flow Cytometry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Indicators and Reagents</topic><topic>Leukemia, Lymphoid</topic><topic>Molecular and cellular biology</topic><topic>RNA - analysis</topic><topic>RNA, Neoplasm - analysis</topic><topic>Staining and Labeling</topic><topic>standardized staining methods</topic><toplevel>online_resources</toplevel><creatorcontrib>Pennings, Arie</creatorcontrib><creatorcontrib>Speth, Paul</creatorcontrib><creatorcontrib>Wessels, Hans</creatorcontrib><creatorcontrib>Haanen, Clemens</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pennings, Arie</au><au>Speth, Paul</au><au>Wessels, Hans</au><au>Haanen, Clemens</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved flow cytometry of cellular DNA and RNA by on‐line reagent addition</atitle><jtitle>Cytometry (New York, N.Y.)</jtitle><addtitle>Cytometry</addtitle><date>1987-05</date><risdate>1987</risdate><volume>8</volume><issue>3</issue><spage>335</spage><epage>338</epage><pages>335-338</pages><issn>0196-4763</issn><eissn>1097-0320</eissn><coden>CYTODQ</coden><abstract>Acridine orange (AO) staining enables flow cytometric measurement of cellular DNA and RNA. With the conventional procedure, red and green fluorescence intensities alter considerably in time, affecting the reliability and reproducibility of the results. A standardized simple technique of AO staining is described that keeps the time between addition of detergent and AO and the subsequent measurement constant. Measurements by means of this on‐line method appear to be much more reproducible in comparison to those obtained using the conventional procedure.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2439265</pmid><doi>10.1002/cyto.990080315</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0196-4763 |
| ispartof | Cytometry (New York, N.Y.), 1987-05, Vol.8 (3), p.335-338 |
| issn | 0196-4763 1097-0320 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Acridine orange Biological and medical sciences Cell Line Diverse techniques DNA - analysis DNA, Neoplasm - analysis Flow Cytometry - instrumentation Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Humans Indicators and Reagents Leukemia, Lymphoid Molecular and cellular biology RNA - analysis RNA, Neoplasm - analysis Staining and Labeling standardized staining methods |
| title | Improved flow cytometry of cellular DNA and RNA by on‐line reagent addition |
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