A 2.4-Megabase Physical Map Spanning the CYP2C Gene Cluster on Chromosome 10q24
The CYP2C gene cluster on chromosome 10q24 encodes the P450IIC enzymes, members of the cytochrome P450 monooxygenase superfamily. The P450-IIC enzymes are required for the metabolism of a number of foreign compounds, including the drugs mephenytoin and tolbutamide, and are also thought to be involve...
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| Veröffentlicht in: | Genomics 1995-07, Vol.28 (2), p.328-332 |
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| creator | Gray, Ian C. Nobile, Carlo Muresu, Rosella Ford, Sally Spurr, Nigel K. |
| description | The CYP2C gene cluster on chromosome 10q24 encodes the P450IIC enzymes, members of the cytochrome P450 monooxygenase superfamily. The P450-IIC enzymes are required for the metabolism of a number of foreign compounds, including the drugs mephenytoin and tolbutamide, and are also thought to be involved in the metabolism of endogenous steroid hormones. Several different CYP2C cDNA clones have been isolated; however, the exact number of genes and the genomic arrangement of the CYP2C cluster have remained unknown. Using a combination of STS and restriction mapping to characterize YAC clones, we have constructed a 2.4-Mb physical map that incorporates the CYP2C gene cluster. The cluster spans approximately 500 kb on proximal 10q24 and comprises four genes arranged in the order CYP2C8-CYP2C9-CYP2C19-CYP2C18 . The map also includes an adjacent gene, the serum retinol binding protein gene (RBP4). The incorporation of Généthon CA repeat genetic markers suggests the orientation of the loci to be Cen-RBP4-CYP2C18-CYP2C19-CYP2C9-CYP2C8-Tel. |
| doi_str_mv | 10.1006/geno.1995.1149 |
| format | Article |
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The P450-IIC enzymes are required for the metabolism of a number of foreign compounds, including the drugs mephenytoin and tolbutamide, and are also thought to be involved in the metabolism of endogenous steroid hormones. Several different CYP2C cDNA clones have been isolated; however, the exact number of genes and the genomic arrangement of the CYP2C cluster have remained unknown. Using a combination of STS and restriction mapping to characterize YAC clones, we have constructed a 2.4-Mb physical map that incorporates the CYP2C gene cluster. The cluster spans approximately 500 kb on proximal 10q24 and comprises four genes arranged in the order CYP2C8-CYP2C9-CYP2C19-CYP2C18 . The map also includes an adjacent gene, the serum retinol binding protein gene (RBP4). The incorporation of Généthon CA repeat genetic markers suggests the orientation of the loci to be Cen-RBP4-CYP2C18-CYP2C19-CYP2C9-CYP2C8-Tel.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1995.1149</identifier><identifier>PMID: 8530044</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Aryl Hydrocarbon Hydroxylases ; Base Sequence ; Biological and medical sciences ; BIOLOGICAL MARKERS ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Chromosome Mapping ; Chromosomes, Human, Pair 10 ; Classical genetics, quantitative genetics, hybrids ; Cytochrome P-450 CYP2C8 ; Cytochrome P-450 CYP2C9 ; Cytochrome P-450 Enzyme System - genetics ; DNA SEQUENCING ; DNA-CLONING ; ELECTROPHORESIS ; ENZYMES ; Fundamental and applied biological sciences. Psychology ; GENES ; GENETIC MAPPING ; Genetics of eukaryotes. Biological and molecular evolution ; Human ; HUMAN CHROMOSOMES ; Humans ; METABOLISM ; Molecular Sequence Data ; Multigene Family ; POLYMERASE CHAIN REACTION ; Retinol-Binding Proteins - genetics ; Sequence Tagged Sites ; Steroid 16-alpha-Hydroxylase ; STEROID HORMONES ; Steroid Hydroxylases - genetics ; STRUCTURE-ACTIVITY RELATIONSHIPS</subject><ispartof>Genomics, 1995-07, Vol.28 (2), p.328-332</ispartof><rights>1995 Academic Press</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-116df07d374825bf90b7e7fe4fcbf56bda50af21f694136d84c4819af7c62cff3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754385711494$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3624731$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8530044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/390939$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Gray, Ian C.</creatorcontrib><creatorcontrib>Nobile, Carlo</creatorcontrib><creatorcontrib>Muresu, Rosella</creatorcontrib><creatorcontrib>Ford, Sally</creatorcontrib><creatorcontrib>Spurr, Nigel K.</creatorcontrib><title>A 2.4-Megabase Physical Map Spanning the CYP2C Gene Cluster on Chromosome 10q24</title><title>Genomics</title><addtitle>Genomics</addtitle><description>The CYP2C gene cluster on chromosome 10q24 encodes the P450IIC enzymes, members of the cytochrome P450 monooxygenase superfamily. The P450-IIC enzymes are required for the metabolism of a number of foreign compounds, including the drugs mephenytoin and tolbutamide, and are also thought to be involved in the metabolism of endogenous steroid hormones. Several different CYP2C cDNA clones have been isolated; however, the exact number of genes and the genomic arrangement of the CYP2C cluster have remained unknown. Using a combination of STS and restriction mapping to characterize YAC clones, we have constructed a 2.4-Mb physical map that incorporates the CYP2C gene cluster. The cluster spans approximately 500 kb on proximal 10q24 and comprises four genes arranged in the order CYP2C8-CYP2C9-CYP2C19-CYP2C18 . The map also includes an adjacent gene, the serum retinol binding protein gene (RBP4). The incorporation of Généthon CA repeat genetic markers suggests the orientation of the loci to be Cen-RBP4-CYP2C18-CYP2C19-CYP2C9-CYP2C8-Tel.</description><subject>Aryl Hydrocarbon Hydroxylases</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MARKERS</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 10</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Cytochrome P-450 CYP2C8</subject><subject>Cytochrome P-450 CYP2C9</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>DNA SEQUENCING</subject><subject>DNA-CLONING</subject><subject>ELECTROPHORESIS</subject><subject>ENZYMES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENES</subject><subject>GENETIC MAPPING</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Human</subject><subject>HUMAN CHROMOSOMES</subject><subject>Humans</subject><subject>METABOLISM</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>Retinol-Binding Proteins - genetics</subject><subject>Sequence Tagged Sites</subject><subject>Steroid 16-alpha-Hydroxylase</subject><subject>STEROID HORMONES</subject><subject>Steroid Hydroxylases - genetics</subject><subject>STRUCTURE-ACTIVITY RELATIONSHIPS</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1r3DAQxUVpSLdpr70VVCi52ZUsWR_HYJq0kJBA20NPQpZHuyq2tJG8hfz3sdklt9LTDMxvhnnvIfSBkpoSIr5sIaaaat3WlHL9Cm0oUbpSgovXaEOUUpVsOXuD3pbyhxCimWrO0blqGSGcb9D9FW5qXt3B1va2AH7YPZXg7Ijv7B7_2NsYQ9zieQe4-_3QdPgG4tKOhzJDxinibpfTlEqaAFPy2PB36MzbscD7U71Av66__uy-Vbf3N9-7q9vKcU3nilIxeCIHJrlq2t5r0kuQHrh3vW9FP9iWWN9QLzSnTAyKO66otl460Tjv2QX6dLybyhxMcWEGt3MpRnCzYXrRqRfm8sjsc3o8QJnNFIqDcbQR0qEYKVtBmZT_BanQireiWcD6CLqcSsngzT6HyeYnQ4lZ4zBrHGaNw6xxLAsfT5cP_QTDC37yf5l_Ps1tWVz32UYXygvGRMMlowumjhgslv4NkFfFEB0MIa-ChxT-9cEzekyitg</recordid><startdate>19950720</startdate><enddate>19950720</enddate><creator>Gray, Ian C.</creator><creator>Nobile, Carlo</creator><creator>Muresu, Rosella</creator><creator>Ford, Sally</creator><creator>Spurr, Nigel K.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19950720</creationdate><title>A 2.4-Megabase Physical Map Spanning the CYP2C Gene Cluster on Chromosome 10q24</title><author>Gray, Ian C. ; Nobile, Carlo ; Muresu, Rosella ; Ford, Sally ; Spurr, Nigel K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-116df07d374825bf90b7e7fe4fcbf56bda50af21f694136d84c4819af7c62cff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Aryl Hydrocarbon Hydroxylases</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MARKERS</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 10</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Cytochrome P-450 CYP2C8</topic><topic>Cytochrome P-450 CYP2C9</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>DNA SEQUENCING</topic><topic>DNA-CLONING</topic><topic>ELECTROPHORESIS</topic><topic>ENZYMES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENES</topic><topic>GENETIC MAPPING</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Human</topic><topic>HUMAN CHROMOSOMES</topic><topic>Humans</topic><topic>METABOLISM</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>Retinol-Binding Proteins - genetics</topic><topic>Sequence Tagged Sites</topic><topic>Steroid 16-alpha-Hydroxylase</topic><topic>STEROID HORMONES</topic><topic>Steroid Hydroxylases - genetics</topic><topic>STRUCTURE-ACTIVITY RELATIONSHIPS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gray, Ian C.</creatorcontrib><creatorcontrib>Nobile, Carlo</creatorcontrib><creatorcontrib>Muresu, Rosella</creatorcontrib><creatorcontrib>Ford, Sally</creatorcontrib><creatorcontrib>Spurr, Nigel K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gray, Ian C.</au><au>Nobile, Carlo</au><au>Muresu, Rosella</au><au>Ford, Sally</au><au>Spurr, Nigel K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A 2.4-Megabase Physical Map Spanning the CYP2C Gene Cluster on Chromosome 10q24</atitle><jtitle>Genomics</jtitle><addtitle>Genomics</addtitle><date>1995-07-20</date><risdate>1995</risdate><volume>28</volume><issue>2</issue><spage>328</spage><epage>332</epage><pages>328-332</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>The CYP2C gene cluster on chromosome 10q24 encodes the P450IIC enzymes, members of the cytochrome P450 monooxygenase superfamily. The P450-IIC enzymes are required for the metabolism of a number of foreign compounds, including the drugs mephenytoin and tolbutamide, and are also thought to be involved in the metabolism of endogenous steroid hormones. Several different CYP2C cDNA clones have been isolated; however, the exact number of genes and the genomic arrangement of the CYP2C cluster have remained unknown. Using a combination of STS and restriction mapping to characterize YAC clones, we have constructed a 2.4-Mb physical map that incorporates the CYP2C gene cluster. The cluster spans approximately 500 kb on proximal 10q24 and comprises four genes arranged in the order CYP2C8-CYP2C9-CYP2C19-CYP2C18 . The map also includes an adjacent gene, the serum retinol binding protein gene (RBP4). The incorporation of Généthon CA repeat genetic markers suggests the orientation of the loci to be Cen-RBP4-CYP2C18-CYP2C19-CYP2C9-CYP2C8-Tel.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8530044</pmid><doi>10.1006/geno.1995.1149</doi><tpages>5</tpages></addata></record> |
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| subjects | Aryl Hydrocarbon Hydroxylases Base Sequence Biological and medical sciences BIOLOGICAL MARKERS BIOLOGY AND MEDICINE, BASIC STUDIES Chromosome Mapping Chromosomes, Human, Pair 10 Classical genetics, quantitative genetics, hybrids Cytochrome P-450 CYP2C8 Cytochrome P-450 CYP2C9 Cytochrome P-450 Enzyme System - genetics DNA SEQUENCING DNA-CLONING ELECTROPHORESIS ENZYMES Fundamental and applied biological sciences. Psychology GENES GENETIC MAPPING Genetics of eukaryotes. Biological and molecular evolution Human HUMAN CHROMOSOMES Humans METABOLISM Molecular Sequence Data Multigene Family POLYMERASE CHAIN REACTION Retinol-Binding Proteins - genetics Sequence Tagged Sites Steroid 16-alpha-Hydroxylase STEROID HORMONES Steroid Hydroxylases - genetics STRUCTURE-ACTIVITY RELATIONSHIPS |
| title | A 2.4-Megabase Physical Map Spanning the CYP2C Gene Cluster on Chromosome 10q24 |
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