STUDIES ON RADIOIMMUNOASSAY OF HUMAN ANIONIC GLUTATHIONE S-TRANSFERASE AND ITS CLINICAL APPLICATION FOR CANCER OF THE DIGESTIVE ORGANS

In the present report, human anionic GST was purified from human placenta by CM-cellulose, Sephadex G100, GSH-Lys-Sepharose 4B and isoelectric forcusing. Using this purified protein as a antigen, specific anti serum was prepared in rabbit. The radioimmunoassay system for anionic GST using double antibody technique showed sufficient specificity, reproducibility and sensitivity for most serum sample. The anionic GST concentration of healthy control sera was 13.52±3.1ng/ml (mean±SD). Marked increase of serum anionic GST was observed in patients with gastric cancer (26.1±14.8ng/ml), colon cancer (44.0±15.1ng/ml), metastatic liver cancer (266.0± 178.3ng/ml) and hepatocellular carcinoma (175.4±55.2ng/ml). These results indicate that radioimmunoassay of anionic GST is useful for serological diagnosis of malignancies from alimentary tract and liver.