Selective Killing of Cholinergic Neurons by Microglial Activation in Basal Forebrain Mixed Neuronal/Glial Cultures
Microglia activation by lipopolysaccharides (LPS) significantly decreased choline acetyltransferase−-immunopositive (ChAT+) neuron number and ChAT activity in rat primary basal forebrain mixed neuronal/glial cultures. The number of non-cholinergic (ChAT(−)) neurons was unaffected. LPS induced nitric...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1995-10, Vol.215 (2), p.572-577 |
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| creator | Mcmillian, M. Kong, L.Y. Sawin, S.M. Wilson, B. Das, K. Hudson, P. Hong, J.S. Bing, G.Y. |
| description | Microglia activation by lipopolysaccharides (LPS) significantly decreased choline acetyltransferase−-immunopositive (ChAT+) neuron number and ChAT activity in rat primary basal forebrain mixed neuronal/glial cultures. The number of non-cholinergic (ChAT(−)) neurons was unaffected. LPS induced nitric oxide synthase (NOS) in microglia, increased the media level of the NO metabolite nitrite, and the NOS inhibitor N
G-nitro-L-arginine methylester (NAME) protected the ChAT+ neurons from LPS. The combination of β-amyloid peptide (1-42) and interferon-γ (INF-γ) also increased the media nitrite level and decreased ChAT+ neuron number. Cholinergic neurons are lost early in the course of Alzheimer′s disease, and the enhanced sensitivity of these neurons to microglial activation in mixed neuronal/glial culture may be useful for modeling Alzheimer′s disease and developing therapeutic strategies to combat this disease. |
| doi_str_mv | 10.1006/bbrc.1995.2503 |
| format | Article |
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G-nitro-L-arginine methylester (NAME) protected the ChAT+ neurons from LPS. The combination of β-amyloid peptide (1-42) and interferon-γ (INF-γ) also increased the media nitrite level and decreased ChAT+ neuron number. Cholinergic neurons are lost early in the course of Alzheimer′s disease, and the enhanced sensitivity of these neurons to microglial activation in mixed neuronal/glial culture may be useful for modeling Alzheimer′s disease and developing therapeutic strategies to combat this disease.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1995.2503</identifier><identifier>PMID: 7487994</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amyloid beta-Peptides - pharmacology ; Animals ; Arginine - analogs & derivatives ; Arginine - pharmacology ; Cell Survival - drug effects ; Cell Survival - physiology ; Cells, Cultured ; Choline O-Acetyltransferase - analysis ; Choline O-Acetyltransferase - metabolism ; Coculture Techniques ; Dizocilpine Maleate - pharmacology ; Embryo, Mammalian ; Enzyme Induction - drug effects ; Interferon-gamma - pharmacology ; Lipopolysaccharides - pharmacology ; Microglia - cytology ; Microglia - drug effects ; Microglia - metabolism ; Neurons - cytology ; Neurons - drug effects ; Neurons - metabolism ; NG-Nitroarginine Methyl Ester ; Nitric Oxide Synthase - biosynthesis ; Prosencephalon - cytology ; Prosencephalon - metabolism ; Rats ; Recombinant Proteins</subject><ispartof>Biochemical and biophysical research communications, 1995-10, Vol.215 (2), p.572-577</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-e79d0ff037ed8c4bfd8898f26b2e489675b8d4502a4298cdf952d640c5d35c373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X8572503X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7487994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mcmillian, M.</creatorcontrib><creatorcontrib>Kong, L.Y.</creatorcontrib><creatorcontrib>Sawin, S.M.</creatorcontrib><creatorcontrib>Wilson, B.</creatorcontrib><creatorcontrib>Das, K.</creatorcontrib><creatorcontrib>Hudson, P.</creatorcontrib><creatorcontrib>Hong, J.S.</creatorcontrib><creatorcontrib>Bing, G.Y.</creatorcontrib><title>Selective Killing of Cholinergic Neurons by Microglial Activation in Basal Forebrain Mixed Neuronal/Glial Cultures</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Microglia activation by lipopolysaccharides (LPS) significantly decreased choline acetyltransferase−-immunopositive (ChAT+) neuron number and ChAT activity in rat primary basal forebrain mixed neuronal/glial cultures. The number of non-cholinergic (ChAT(−)) neurons was unaffected. LPS induced nitric oxide synthase (NOS) in microglia, increased the media level of the NO metabolite nitrite, and the NOS inhibitor N
G-nitro-L-arginine methylester (NAME) protected the ChAT+ neurons from LPS. The combination of β-amyloid peptide (1-42) and interferon-γ (INF-γ) also increased the media nitrite level and decreased ChAT+ neuron number. Cholinergic neurons are lost early in the course of Alzheimer′s disease, and the enhanced sensitivity of these neurons to microglial activation in mixed neuronal/glial culture may be useful for modeling Alzheimer′s disease and developing therapeutic strategies to combat this disease.</description><subject>Amyloid beta-Peptides - pharmacology</subject><subject>Animals</subject><subject>Arginine - analogs & derivatives</subject><subject>Arginine - pharmacology</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - physiology</subject><subject>Cells, Cultured</subject><subject>Choline O-Acetyltransferase - analysis</subject><subject>Choline O-Acetyltransferase - metabolism</subject><subject>Coculture Techniques</subject><subject>Dizocilpine Maleate - pharmacology</subject><subject>Embryo, Mammalian</subject><subject>Enzyme Induction - drug effects</subject><subject>Interferon-gamma - pharmacology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Microglia - cytology</subject><subject>Microglia - drug effects</subject><subject>Microglia - metabolism</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>NG-Nitroarginine Methyl Ester</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Prosencephalon - cytology</subject><subject>Prosencephalon - metabolism</subject><subject>Rats</subject><subject>Recombinant Proteins</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1v3CAQxVHVaLtJe-2tEqfevAEMNhzTVb6Ur0MaKTeEYbylYk0KdtT974u7q9xyGoZ5743mh9BXSlaUkOa065JdUaXEiglSf0BLShSpGCX8I1qSoqiYos-f0HHOvwmhlDdqgRYtl61SfInSIwSwo38FfOND8MMGxx6vf8XyhLTxFt_DlOKQcbfDd96muAneBHw2e8zo44D9gH-YXP4uYoIumdLf-b_gDk4TTi__W9ZTGKcE-TM66k3I8OVQT9DTxfnP9VV1-3B5vT67rWxdq7GCVjnS96RuwUnLu95JqWTPmo4Bl6ppRScdF4QZzpS0rleCuYYTK1wtbN3WJ-j7PvclxT8T5FFvfbYQghkgTlm3rRCsrCrC1V5Yrss5Qa9fkt-atNOU6BmyniHrGbKeIRfDt0Py1G3BvckPVMtc7udQznv1kHS2HgYLzqcCW7vo34v-B2rTjJs</recordid><startdate>19951013</startdate><enddate>19951013</enddate><creator>Mcmillian, M.</creator><creator>Kong, L.Y.</creator><creator>Sawin, S.M.</creator><creator>Wilson, B.</creator><creator>Das, K.</creator><creator>Hudson, P.</creator><creator>Hong, J.S.</creator><creator>Bing, G.Y.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951013</creationdate><title>Selective Killing of Cholinergic Neurons by Microglial Activation in Basal Forebrain Mixed Neuronal/Glial Cultures</title><author>Mcmillian, M. ; Kong, L.Y. ; Sawin, S.M. ; Wilson, B. ; Das, K. ; Hudson, P. ; Hong, J.S. ; Bing, G.Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-e79d0ff037ed8c4bfd8898f26b2e489675b8d4502a4298cdf952d640c5d35c373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amyloid beta-Peptides - pharmacology</topic><topic>Animals</topic><topic>Arginine - analogs & derivatives</topic><topic>Arginine - pharmacology</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - physiology</topic><topic>Cells, Cultured</topic><topic>Choline O-Acetyltransferase - analysis</topic><topic>Choline O-Acetyltransferase - metabolism</topic><topic>Coculture Techniques</topic><topic>Dizocilpine Maleate - pharmacology</topic><topic>Embryo, Mammalian</topic><topic>Enzyme Induction - drug effects</topic><topic>Interferon-gamma - pharmacology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Microglia - cytology</topic><topic>Microglia - drug effects</topic><topic>Microglia - metabolism</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>NG-Nitroarginine Methyl Ester</topic><topic>Nitric Oxide Synthase - biosynthesis</topic><topic>Prosencephalon - cytology</topic><topic>Prosencephalon - metabolism</topic><topic>Rats</topic><topic>Recombinant Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mcmillian, M.</creatorcontrib><creatorcontrib>Kong, L.Y.</creatorcontrib><creatorcontrib>Sawin, S.M.</creatorcontrib><creatorcontrib>Wilson, B.</creatorcontrib><creatorcontrib>Das, K.</creatorcontrib><creatorcontrib>Hudson, P.</creatorcontrib><creatorcontrib>Hong, J.S.</creatorcontrib><creatorcontrib>Bing, G.Y.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mcmillian, M.</au><au>Kong, L.Y.</au><au>Sawin, S.M.</au><au>Wilson, B.</au><au>Das, K.</au><au>Hudson, P.</au><au>Hong, J.S.</au><au>Bing, G.Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective Killing of Cholinergic Neurons by Microglial Activation in Basal Forebrain Mixed Neuronal/Glial Cultures</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1995-10-13</date><risdate>1995</risdate><volume>215</volume><issue>2</issue><spage>572</spage><epage>577</epage><pages>572-577</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Microglia activation by lipopolysaccharides (LPS) significantly decreased choline acetyltransferase−-immunopositive (ChAT+) neuron number and ChAT activity in rat primary basal forebrain mixed neuronal/glial cultures. The number of non-cholinergic (ChAT(−)) neurons was unaffected. LPS induced nitric oxide synthase (NOS) in microglia, increased the media level of the NO metabolite nitrite, and the NOS inhibitor N
G-nitro-L-arginine methylester (NAME) protected the ChAT+ neurons from LPS. The combination of β-amyloid peptide (1-42) and interferon-γ (INF-γ) also increased the media nitrite level and decreased ChAT+ neuron number. Cholinergic neurons are lost early in the course of Alzheimer′s disease, and the enhanced sensitivity of these neurons to microglial activation in mixed neuronal/glial culture may be useful for modeling Alzheimer′s disease and developing therapeutic strategies to combat this disease.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7487994</pmid><doi>10.1006/bbrc.1995.2503</doi><tpages>6</tpages></addata></record> |
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| subjects | Amyloid beta-Peptides - pharmacology Animals Arginine - analogs & derivatives Arginine - pharmacology Cell Survival - drug effects Cell Survival - physiology Cells, Cultured Choline O-Acetyltransferase - analysis Choline O-Acetyltransferase - metabolism Coculture Techniques Dizocilpine Maleate - pharmacology Embryo, Mammalian Enzyme Induction - drug effects Interferon-gamma - pharmacology Lipopolysaccharides - pharmacology Microglia - cytology Microglia - drug effects Microglia - metabolism Neurons - cytology Neurons - drug effects Neurons - metabolism NG-Nitroarginine Methyl Ester Nitric Oxide Synthase - biosynthesis Prosencephalon - cytology Prosencephalon - metabolism Rats Recombinant Proteins |
| title | Selective Killing of Cholinergic Neurons by Microglial Activation in Basal Forebrain Mixed Neuronal/Glial Cultures |
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