Acquired retinochoroiditis in hamsters inoculated with ME 49 strain Toxoplasma
These studies were undertaken to establish an animal model for use in studies of ocular toxoplasmosis. An animal model is needed to examine the development, progression, and resolution of ocular Toxoplasma infections and to study the effects on the disease of currently used and experimental therapie...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 1995-10, Vol.36 (11), p.2166-2175 |
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| creator | Pavesio, CE Chiappino, ML Gormley, P Setzer, PY Nichols, BA |
| description | These studies were undertaken to establish an animal model for use in studies of ocular toxoplasmosis. An animal model is needed to examine the development, progression, and resolution of ocular Toxoplasma infections and to study the effects on the disease of currently used and experimental therapies.
Cysts of the ME 49 strain of Toxoplasma gondii were injected intraperitoneally into each of 60 golden hamsters. The hamsters' eyes were examined before inoculation and at intervals after inoculation, and fundus photographs were taken. Histologic sections were analyzed and photographed to document the ocular effects of the infection.
Retinochoroiditis was found in both eyes of all hamsters within 2 to 3 weeks of inoculation. The disease resolved spontaneously without treatment and was quiescent in most cases at 12 weeks after inoculation. The animals remained in good general health, and those tested had high antibody titers to Toxoplasma (1:256 to 1:32,000) at 6 months after the infection. The discovery of cysts and lesions in the retina confirmed the diagnosis.
Although the lesions were not identical to those of human disease, this animal model of ocular toxoplasmosis offers several advantages: reproducibility, short incubation time, spontaneous resolution without treatment, consistent production of cysts, and ease of inoculation intraperitoneally without intraocular injection. |
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Cysts of the ME 49 strain of Toxoplasma gondii were injected intraperitoneally into each of 60 golden hamsters. The hamsters' eyes were examined before inoculation and at intervals after inoculation, and fundus photographs were taken. Histologic sections were analyzed and photographed to document the ocular effects of the infection.
Retinochoroiditis was found in both eyes of all hamsters within 2 to 3 weeks of inoculation. The disease resolved spontaneously without treatment and was quiescent in most cases at 12 weeks after inoculation. The animals remained in good general health, and those tested had high antibody titers to Toxoplasma (1:256 to 1:32,000) at 6 months after the infection. The discovery of cysts and lesions in the retina confirmed the diagnosis.
Although the lesions were not identical to those of human disease, this animal model of ocular toxoplasmosis offers several advantages: reproducibility, short incubation time, spontaneous resolution without treatment, consistent production of cysts, and ease of inoculation intraperitoneally without intraocular injection.</description><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>PMID: 7558709</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Animals ; Antibodies, Protozoan - analysis ; Biological and medical sciences ; Brain - parasitology ; Chorioretinitis - parasitology ; Chorioretinitis - pathology ; Chorioretinitis - physiopathology ; Choroiditis - parasitology ; Choroiditis - pathology ; Choroiditis - physiopathology ; Cricetinae ; Disease Models, Animal ; Experimental protozoal diseases and models ; Fundus Oculi ; Humans ; Immunoenzyme Techniques ; Infectious diseases ; Medical sciences ; Mesocricetus ; Mice ; Parasitic diseases ; Protozoal diseases ; Retina - parasitology ; Retina - pathology ; Specific Pathogen-Free Organisms ; Toxoplasma - immunology ; Toxoplasma - isolation & purification ; Toxoplasma - physiology ; Toxoplasmosis, Animal - etiology ; Toxoplasmosis, Animal - pathology ; Toxoplasmosis, Animal - physiopathology ; Toxoplasmosis, Ocular - etiology ; Toxoplasmosis, Ocular - pathology ; Toxoplasmosis, Ocular - physiopathology</subject><ispartof>Investigative ophthalmology & visual science, 1995-10, Vol.36 (11), p.2166-2175</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3688666$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7558709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pavesio, CE</creatorcontrib><creatorcontrib>Chiappino, ML</creatorcontrib><creatorcontrib>Gormley, P</creatorcontrib><creatorcontrib>Setzer, PY</creatorcontrib><creatorcontrib>Nichols, BA</creatorcontrib><title>Acquired retinochoroiditis in hamsters inoculated with ME 49 strain Toxoplasma</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>These studies were undertaken to establish an animal model for use in studies of ocular toxoplasmosis. An animal model is needed to examine the development, progression, and resolution of ocular Toxoplasma infections and to study the effects on the disease of currently used and experimental therapies.
Cysts of the ME 49 strain of Toxoplasma gondii were injected intraperitoneally into each of 60 golden hamsters. The hamsters' eyes were examined before inoculation and at intervals after inoculation, and fundus photographs were taken. Histologic sections were analyzed and photographed to document the ocular effects of the infection.
Retinochoroiditis was found in both eyes of all hamsters within 2 to 3 weeks of inoculation. The disease resolved spontaneously without treatment and was quiescent in most cases at 12 weeks after inoculation. The animals remained in good general health, and those tested had high antibody titers to Toxoplasma (1:256 to 1:32,000) at 6 months after the infection. The discovery of cysts and lesions in the retina confirmed the diagnosis.
Although the lesions were not identical to those of human disease, this animal model of ocular toxoplasmosis offers several advantages: reproducibility, short incubation time, spontaneous resolution without treatment, consistent production of cysts, and ease of inoculation intraperitoneally without intraocular injection.</description><subject>Animals</subject><subject>Antibodies, Protozoan - analysis</subject><subject>Biological and medical sciences</subject><subject>Brain - parasitology</subject><subject>Chorioretinitis - parasitology</subject><subject>Chorioretinitis - pathology</subject><subject>Chorioretinitis - physiopathology</subject><subject>Choroiditis - parasitology</subject><subject>Choroiditis - pathology</subject><subject>Choroiditis - physiopathology</subject><subject>Cricetinae</subject><subject>Disease Models, Animal</subject><subject>Experimental protozoal diseases and models</subject><subject>Fundus Oculi</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mesocricetus</subject><subject>Mice</subject><subject>Parasitic diseases</subject><subject>Protozoal diseases</subject><subject>Retina - parasitology</subject><subject>Retina - pathology</subject><subject>Specific Pathogen-Free Organisms</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma - isolation & purification</subject><subject>Toxoplasma - physiology</subject><subject>Toxoplasmosis, Animal - etiology</subject><subject>Toxoplasmosis, Animal - pathology</subject><subject>Toxoplasmosis, Animal - physiopathology</subject><subject>Toxoplasmosis, Ocular - etiology</subject><subject>Toxoplasmosis, Ocular - pathology</subject><subject>Toxoplasmosis, Ocular - physiopathology</subject><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo90F1LwzAUBuAgypzTnyD0QvSqkO-0l2PMD5h6M69DmqY20jZbklL990ZWvDoHzsML5z0DS8QYzpkoyDlYQkR5Dimkl-AqhC8IMUIYLsBCMFYIWC7B21ofR-tNnXkT7eB067yztY02ZHbIWtWHaPzf7vTYqZjgZGObvW4zWmYhepXU3n27Q6dCr67BRaO6YG7muQIfj9v95jnfvT-9bNa7vMVcxJxQhiukaKFUZYhgnDAjTAOVZkhUQhBDCi1wCQkiBSEMlwRTVEFTIVw3pCQrcH_KPXh3HE2IsrdBm65Tg3FjkEIwWkKGE7yd4Vj1ppYHb3vlf-RcQLrfzXcVtOoarwZtwz8jvCg454k9nFhrP9sp9SXTs12XQpGcpolwiZDEKMlfJy1xQg</recordid><startdate>19951001</startdate><enddate>19951001</enddate><creator>Pavesio, CE</creator><creator>Chiappino, ML</creator><creator>Gormley, P</creator><creator>Setzer, PY</creator><creator>Nichols, BA</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19951001</creationdate><title>Acquired retinochoroiditis in hamsters inoculated with ME 49 strain Toxoplasma</title><author>Pavesio, CE ; Chiappino, ML ; Gormley, P ; Setzer, PY ; Nichols, BA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-3452b1a48aabe375635e7ef0ac517b773e38c72903138335293241b0eb12df393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Antibodies, Protozoan - analysis</topic><topic>Biological and medical sciences</topic><topic>Brain - parasitology</topic><topic>Chorioretinitis - parasitology</topic><topic>Chorioretinitis - pathology</topic><topic>Chorioretinitis - physiopathology</topic><topic>Choroiditis - parasitology</topic><topic>Choroiditis - pathology</topic><topic>Choroiditis - physiopathology</topic><topic>Cricetinae</topic><topic>Disease Models, Animal</topic><topic>Experimental protozoal diseases and models</topic><topic>Fundus Oculi</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Mesocricetus</topic><topic>Mice</topic><topic>Parasitic diseases</topic><topic>Protozoal diseases</topic><topic>Retina - parasitology</topic><topic>Retina - pathology</topic><topic>Specific Pathogen-Free Organisms</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma - isolation & purification</topic><topic>Toxoplasma - physiology</topic><topic>Toxoplasmosis, Animal - etiology</topic><topic>Toxoplasmosis, Animal - pathology</topic><topic>Toxoplasmosis, Animal - physiopathology</topic><topic>Toxoplasmosis, Ocular - etiology</topic><topic>Toxoplasmosis, Ocular - pathology</topic><topic>Toxoplasmosis, Ocular - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pavesio, CE</creatorcontrib><creatorcontrib>Chiappino, ML</creatorcontrib><creatorcontrib>Gormley, P</creatorcontrib><creatorcontrib>Setzer, PY</creatorcontrib><creatorcontrib>Nichols, BA</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pavesio, CE</au><au>Chiappino, ML</au><au>Gormley, P</au><au>Setzer, PY</au><au>Nichols, BA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acquired retinochoroiditis in hamsters inoculated with ME 49 strain Toxoplasma</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>1995-10-01</date><risdate>1995</risdate><volume>36</volume><issue>11</issue><spage>2166</spage><epage>2175</epage><pages>2166-2175</pages><issn>0146-0404</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>These studies were undertaken to establish an animal model for use in studies of ocular toxoplasmosis. An animal model is needed to examine the development, progression, and resolution of ocular Toxoplasma infections and to study the effects on the disease of currently used and experimental therapies.
Cysts of the ME 49 strain of Toxoplasma gondii were injected intraperitoneally into each of 60 golden hamsters. The hamsters' eyes were examined before inoculation and at intervals after inoculation, and fundus photographs were taken. Histologic sections were analyzed and photographed to document the ocular effects of the infection.
Retinochoroiditis was found in both eyes of all hamsters within 2 to 3 weeks of inoculation. The disease resolved spontaneously without treatment and was quiescent in most cases at 12 weeks after inoculation. The animals remained in good general health, and those tested had high antibody titers to Toxoplasma (1:256 to 1:32,000) at 6 months after the infection. The discovery of cysts and lesions in the retina confirmed the diagnosis.
Although the lesions were not identical to those of human disease, this animal model of ocular toxoplasmosis offers several advantages: reproducibility, short incubation time, spontaneous resolution without treatment, consistent production of cysts, and ease of inoculation intraperitoneally without intraocular injection.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>7558709</pmid><tpages>10</tpages></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antibodies, Protozoan - analysis Biological and medical sciences Brain - parasitology Chorioretinitis - parasitology Chorioretinitis - pathology Chorioretinitis - physiopathology Choroiditis - parasitology Choroiditis - pathology Choroiditis - physiopathology Cricetinae Disease Models, Animal Experimental protozoal diseases and models Fundus Oculi Humans Immunoenzyme Techniques Infectious diseases Medical sciences Mesocricetus Mice Parasitic diseases Protozoal diseases Retina - parasitology Retina - pathology Specific Pathogen-Free Organisms Toxoplasma - immunology Toxoplasma - isolation & purification Toxoplasma - physiology Toxoplasmosis, Animal - etiology Toxoplasmosis, Animal - pathology Toxoplasmosis, Animal - physiopathology Toxoplasmosis, Ocular - etiology Toxoplasmosis, Ocular - pathology Toxoplasmosis, Ocular - physiopathology |
| title | Acquired retinochoroiditis in hamsters inoculated with ME 49 strain Toxoplasma |
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