The cytostatic activity of 5-(1-azidovinyl)-2′-deoxyuridine (AzVDU) against herpes simplex virus thymidine kinase gene-transfected FM3A cells is due to inhibition of thymidylate synthase and enhanced by UV light ( λ = 254 nm) exposure
5-(1-Azidovinyl)-2′-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of β- d-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 an...
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| Veröffentlicht in: | FEBS letters 1995-10, Vol.373 (1), p.41-44 |
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| creator | Balzarini, J. Andrei, G. Kumar, R. Knaus, E.E. Wiebe, L.I. De Clercq, E. |
| description | 5-(1-Azidovinyl)-2′-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of β-
d-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. None of the compounds were cytostatic. However, AzVDU, but not the 5-[1-azido-2-halogenoethyl]-AU derivatives became highly cytostatic against HSV-1 and HSV-2 TK genetransfected FM3A tumor cells. The molecular target for the cytostatic effect of AzVDU proved to be thymidylate synthase. Short exposure of AzVDU-treated FM3A TK
−/HSV-1 TK
+ cells to irradiation at
λ = 254 nm enhanced the cytostatic activity of AzVDU by 5-fold. |
| doi_str_mv | 10.1016/0014-5793(95)00994-K |
| format | Article |
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d-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. None of the compounds were cytostatic. However, AzVDU, but not the 5-[1-azido-2-halogenoethyl]-AU derivatives became highly cytostatic against HSV-1 and HSV-2 TK genetransfected FM3A tumor cells. The molecular target for the cytostatic effect of AzVDU proved to be thymidylate synthase. Short exposure of AzVDU-treated FM3A TK
−/HSV-1 TK
+ cells to irradiation at
λ = 254 nm enhanced the cytostatic activity of AzVDU by 5-fold.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(95)00994-K</identifier><identifier>PMID: 7589430</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Antineoplastic Agents - pharmacology ; Antiviral Agents - pharmacology ; Azides - pharmacology ; Azidovinyldeoxyuridine ; Cell Division - drug effects ; Deoxyuridine - analogs & derivatives ; Deoxyuridine - pharmacology ; Gene therapy ; Herpes simplex virus ; Herpesvirus 3, Human - drug effects ; Herpesvirus 3, Human - physiology ; Mammary Neoplasms, Experimental ; Mice ; Mice, Inbred C3H ; Molecular Structure ; Recombinant Proteins - biosynthesis ; Simplexvirus - drug effects ; Simplexvirus - genetics ; Simplexvirus - physiology ; Structure-Activity Relationship ; Thymidine kinase ; Thymidine Kinase - biosynthesis ; Thymidylate synthase ; Transfection ; Tumor Cells, Cultured ; varicella-zoster virus ; Virus Replication - drug effects</subject><ispartof>FEBS letters, 1995-10, Vol.373 (1), p.41-44</ispartof><rights>1995</rights><rights>FEBS Letters 373 (1995) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451K-351aa15d7cd42a0864b44961fd887a2f19c68a66b609f27ba263a133f91abbf53</citedby><cites>FETCH-LOGICAL-c451K-351aa15d7cd42a0864b44961fd887a2f19c68a66b609f27ba263a133f91abbf53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457939500994K$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7589430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Balzarini, J.</creatorcontrib><creatorcontrib>Andrei, G.</creatorcontrib><creatorcontrib>Kumar, R.</creatorcontrib><creatorcontrib>Knaus, E.E.</creatorcontrib><creatorcontrib>Wiebe, L.I.</creatorcontrib><creatorcontrib>De Clercq, E.</creatorcontrib><title>The cytostatic activity of 5-(1-azidovinyl)-2′-deoxyuridine (AzVDU) against herpes simplex virus thymidine kinase gene-transfected FM3A cells is due to inhibition of thymidylate synthase and enhanced by UV light ( λ = 254 nm) exposure</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>5-(1-Azidovinyl)-2′-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of β-
d-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. None of the compounds were cytostatic. However, AzVDU, but not the 5-[1-azido-2-halogenoethyl]-AU derivatives became highly cytostatic against HSV-1 and HSV-2 TK genetransfected FM3A tumor cells. The molecular target for the cytostatic effect of AzVDU proved to be thymidylate synthase. Short exposure of AzVDU-treated FM3A TK
−/HSV-1 TK
+ cells to irradiation at
λ = 254 nm enhanced the cytostatic activity of AzVDU by 5-fold.</description><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Antiviral Agents - pharmacology</subject><subject>Azides - pharmacology</subject><subject>Azidovinyldeoxyuridine</subject><subject>Cell Division - drug effects</subject><subject>Deoxyuridine - analogs & derivatives</subject><subject>Deoxyuridine - pharmacology</subject><subject>Gene therapy</subject><subject>Herpes simplex virus</subject><subject>Herpesvirus 3, Human - drug effects</subject><subject>Herpesvirus 3, Human - physiology</subject><subject>Mammary Neoplasms, Experimental</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Molecular Structure</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Simplexvirus - drug effects</subject><subject>Simplexvirus - genetics</subject><subject>Simplexvirus - physiology</subject><subject>Structure-Activity Relationship</subject><subject>Thymidine kinase</subject><subject>Thymidine Kinase - biosynthesis</subject><subject>Thymidylate synthase</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><subject>varicella-zoster virus</subject><subject>Virus Replication - drug effects</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksGO0zAQhiMEWsrCG4A0J9QeAnZiJ_GBlcqyBdRFXLZ7tZxk0hgSp8ROafbEM3HlHXgI7rwDyabaI3CyPPPPN2P_43lPKXlBCY1eEkKZz2MRzgVfECIE89f3vBlN4tAPWZTc92Z3kofeI2s_keGeUHHincQ8ESwkM-_3VYmQ9a6xTjmdgcqc3mvXQ1MA9-fUVzc6b_ba9NXCD359--7n2Bz6rtW5Ngjz5c31m80C1FZpYx2U2O7QgtX1rsID7HXbWXBlX0_yz9ooi7BFg75rlbEFZg5zWH0Il5BhVVnQFvIOwTWgTalT7XRjxmEmSF8ph2B748oRpEwOaEplsgGS9rC5hkpvSwdz-PkDXkHAGZh6AXjYNbZr8bH3oFCVxSfH89TbrC6uzt_5lx_fvj9fXvoZ43Tth5wqRXkeZzkLFEkiljImIlrkSRKroKAiixIVRWlERBHEqQqiUNEwLARVaVrw8NR7PnF3bfOlQ-tkre34PmWw6ayMY86CJKD_FNKYRIKxkcgmYdY21rZYyF2ra9X2khI5roMcvZaj11JwebsOcj2UPTvyu7TG_K7o6P-QX035r7rC_r-YcnXxOhgTY1zw2-jY6GwC4fCte42ttJnG0RfdDh7LvNF_n_QPTL_b1A</recordid><startdate>19951002</startdate><enddate>19951002</enddate><creator>Balzarini, J.</creator><creator>Andrei, G.</creator><creator>Kumar, R.</creator><creator>Knaus, E.E.</creator><creator>Wiebe, L.I.</creator><creator>De Clercq, E.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19951002</creationdate><title>The cytostatic activity of 5-(1-azidovinyl)-2′-deoxyuridine (AzVDU) against herpes simplex virus thymidine kinase gene-transfected FM3A cells is due to inhibition of thymidylate synthase and enhanced by UV light ( λ = 254 nm) exposure</title><author>Balzarini, J. ; Andrei, G. ; Kumar, R. ; Knaus, E.E. ; Wiebe, L.I. ; De Clercq, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451K-351aa15d7cd42a0864b44961fd887a2f19c68a66b609f27ba263a133f91abbf53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Antiviral Agents - pharmacology</topic><topic>Azides - pharmacology</topic><topic>Azidovinyldeoxyuridine</topic><topic>Cell Division - drug effects</topic><topic>Deoxyuridine - analogs & derivatives</topic><topic>Deoxyuridine - pharmacology</topic><topic>Gene therapy</topic><topic>Herpes simplex virus</topic><topic>Herpesvirus 3, Human - drug effects</topic><topic>Herpesvirus 3, Human - physiology</topic><topic>Mammary Neoplasms, Experimental</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Molecular Structure</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Simplexvirus - drug effects</topic><topic>Simplexvirus - genetics</topic><topic>Simplexvirus - physiology</topic><topic>Structure-Activity Relationship</topic><topic>Thymidine kinase</topic><topic>Thymidine Kinase - biosynthesis</topic><topic>Thymidylate synthase</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><topic>varicella-zoster virus</topic><topic>Virus Replication - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Balzarini, J.</creatorcontrib><creatorcontrib>Andrei, G.</creatorcontrib><creatorcontrib>Kumar, R.</creatorcontrib><creatorcontrib>Knaus, E.E.</creatorcontrib><creatorcontrib>Wiebe, L.I.</creatorcontrib><creatorcontrib>De Clercq, E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Balzarini, J.</au><au>Andrei, G.</au><au>Kumar, R.</au><au>Knaus, E.E.</au><au>Wiebe, L.I.</au><au>De Clercq, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cytostatic activity of 5-(1-azidovinyl)-2′-deoxyuridine (AzVDU) against herpes simplex virus thymidine kinase gene-transfected FM3A cells is due to inhibition of thymidylate synthase and enhanced by UV light ( λ = 254 nm) exposure</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1995-10-02</date><risdate>1995</risdate><volume>373</volume><issue>1</issue><spage>41</spage><epage>44</epage><pages>41-44</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>5-(1-Azidovinyl)-2′-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of β-
d-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. None of the compounds were cytostatic. However, AzVDU, but not the 5-[1-azido-2-halogenoethyl]-AU derivatives became highly cytostatic against HSV-1 and HSV-2 TK genetransfected FM3A tumor cells. The molecular target for the cytostatic effect of AzVDU proved to be thymidylate synthase. Short exposure of AzVDU-treated FM3A TK
−/HSV-1 TK
+ cells to irradiation at
λ = 254 nm enhanced the cytostatic activity of AzVDU by 5-fold.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>7589430</pmid><doi>10.1016/0014-5793(95)00994-K</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1995-10, Vol.373 (1), p.41-44 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77542821 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antineoplastic Agents - pharmacology Antiviral Agents - pharmacology Azides - pharmacology Azidovinyldeoxyuridine Cell Division - drug effects Deoxyuridine - analogs & derivatives Deoxyuridine - pharmacology Gene therapy Herpes simplex virus Herpesvirus 3, Human - drug effects Herpesvirus 3, Human - physiology Mammary Neoplasms, Experimental Mice Mice, Inbred C3H Molecular Structure Recombinant Proteins - biosynthesis Simplexvirus - drug effects Simplexvirus - genetics Simplexvirus - physiology Structure-Activity Relationship Thymidine kinase Thymidine Kinase - biosynthesis Thymidylate synthase Transfection Tumor Cells, Cultured varicella-zoster virus Virus Replication - drug effects |
| title | The cytostatic activity of 5-(1-azidovinyl)-2′-deoxyuridine (AzVDU) against herpes simplex virus thymidine kinase gene-transfected FM3A cells is due to inhibition of thymidylate synthase and enhanced by UV light ( λ = 254 nm) exposure |
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