Development of radioimmunoassays for human erythropoietin using recombinant erythropoietin as tracer and immunogen

Since the estimation of erythropoietin (EPO) by radioimmunoassay (RIA) has been limited by the availability of highly purified urinary (U) human (Hu) EPO, we investigated the use of recombinant (R)-HuEPO as a replacement for U-HuEPO in the preparation of 125I-tracer and high affinity antisera. In ea...

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Veröffentlicht in:	Journal of immunological methods 1987-05, Vol.99 (2), p.235-241
Hauptverfasser:	Egrie, J.C., Cotes, P.Mary, Lane, J., Das, R.E.Gaines, Tam, R.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biological Assay Dose-Response Relationship, Immunologic Erythropoiesis Erythropoietin Erythropoietin - analysis Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Molecular immunology Radioimmunoassay Radioimmunoassay - methods Recombinant DNA Recombinant Proteins Techniques
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