Alternative mechanisms of CAK assembly require an assembly factor or an Activating Kinase

We have cloned a mouse cDNA that encodes p36, a novel subunit of the CDK-activating kinase (CAK). p36 contains a C 3HC 4 zinc-binding domain or RING finger and is associated both with a TFIIH-bound form of CAK and with a free trimeric form. p36 promotes the assembly of CDK7 and cyclin H in vitro, st...

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Veröffentlicht in:Cell 1995-10, Vol.83 (1), p.47-57
Hauptverfasser: Fisher, Robert P., Jin, Pei, Chamberlin, Holly M., Morgan, David O.
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container_title Cell
container_volume 83
creator Fisher, Robert P.
Jin, Pei
Chamberlin, Holly M.
Morgan, David O.
description We have cloned a mouse cDNA that encodes p36, a novel subunit of the CDK-activating kinase (CAK). p36 contains a C 3HC 4 zinc-binding domain or RING finger and is associated both with a TFIIH-bound form of CAK and with a free trimeric form. p36 promotes the assembly of CDK7 and cyclin H in vitro, stabilizing the transient CDK7-cyclin H complex. Stabilization and activation of CAK by p36 is independent of the phosphorylation state of T170, the conserved activating residue of CDK7. Assembly of active CDK7-cyclin H dimers can also occur through an alternative p36-independent pathway that requires phosphorylation of T170 by a CAK-activating kinase, or CAKAK. Thus, CDK7-cyclin H complex formation can be achieved by multiple mechanisms.
doi_str_mv 10.1016/0092-8674(95)90233-3
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subjects Amino Acid Sequence
Animals
Base Sequence
Cyclin H
Cyclin-Dependent Kinases
Cyclins - metabolism
DNA, Complementary - genetics
Feedback
Mice
Molecular Sequence Data
Protein Conformation
Protein-Serine-Threonine Kinases - metabolism
Sequence Alignment
title Alternative mechanisms of CAK assembly require an assembly factor or an Activating Kinase
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