Clonal activation of cytotoxic lymphocyte precursors by monoclonal anti-CD3 antibody: analysis of feeder cell requirements
Activation of cytotoxic lymphocyte precursors (CLP) by the mitogenic monoclonal anti-CD3 antibody OKT3 was studied under limiting dilution (LD) culture conditions. One out of 2–6 E-rosette-purified T cells gave rise to a cytotoxic T cell (CTL) clone when cultured in the presence of OKT3 (0.2–2 ng/ml...
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| Veröffentlicht in: | Immunology letters 1987, Vol.14 (2), p.121-125 |
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| container_end_page | 125 |
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| container_title | Immunology letters |
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| creator | Brucker, Cosima Reimann, Jörg Wagner, Hermann Kabelitz, Dieter |
| description | Activation of cytotoxic lymphocyte precursors (CLP) by the mitogenic monoclonal anti-CD3 antibody OKT3 was studied under limiting dilution (LD) culture conditions. One out of 2–6 E-rosette-purified T cells gave rise to a cytotoxic T cell (CTL) clone when cultured in the presence of OKT3 (0.2–2 ng/ml), recombinant IL-2 (100 U/ml), and irradiated feeder cells. Clonal CLP activation was optimally supported by a combination of E-rosette-depleted non-T feeder cells with small numbers of T cells added back. Among the cell lines tested, Fc-receptor-bearing monocytic cell lines U937 and HL-60 were efficient feeder cells whereas T cell lines (Jurkat, Molt-4, Ke37) did not support clonal CLP activation. These data indicate that clonal activation of CLP and differentiation into cytotoxic effector cells under LD culture conditions are critically influenced by the type and number of feeder cells used. |
| doi_str_mv | 10.1016/0165-2478(87)90090-3 |
| format | Article |
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One out of 2–6 E-rosette-purified T cells gave rise to a cytotoxic T cell (CTL) clone when cultured in the presence of OKT3 (0.2–2 ng/ml), recombinant IL-2 (100 U/ml), and irradiated feeder cells. Clonal CLP activation was optimally supported by a combination of E-rosette-depleted non-T feeder cells with small numbers of T cells added back. Among the cell lines tested, Fc-receptor-bearing monocytic cell lines U937 and HL-60 were efficient feeder cells whereas T cell lines (Jurkat, Molt-4, Ke37) did not support clonal CLP activation. 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One out of 2–6 E-rosette-purified T cells gave rise to a cytotoxic T cell (CTL) clone when cultured in the presence of OKT3 (0.2–2 ng/ml), recombinant IL-2 (100 U/ml), and irradiated feeder cells. Clonal CLP activation was optimally supported by a combination of E-rosette-depleted non-T feeder cells with small numbers of T cells added back. Among the cell lines tested, Fc-receptor-bearing monocytic cell lines U937 and HL-60 were efficient feeder cells whereas T cell lines (Jurkat, Molt-4, Ke37) did not support clonal CLP activation. These data indicate that clonal activation of CLP and differentiation into cytotoxic effector cells under LD culture conditions are critically influenced by the type and number of feeder cells used.</description><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Antibody Specificity</subject><subject>Antigens, Differentiation, T-Lymphocyte</subject><subject>Antigens, Surface - immunology</subject><subject>Applied sciences</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Clonal activation</subject><subject>Cytotoxic lymphocyte precursor</subject><subject>Exact sciences and technology</subject><subject>Feeder cell requirement</subject><subject>Humans</subject><subject>Interleukin-2 - pharmacology</subject><subject>Lymphocyte Activation</subject><subject>Lymphocyte Cooperation - drug effects</subject><subject>Monoclonal anti-CD3 antibody</subject><subject>Other techniques and industries</subject><subject>T-Lymphocytes, Cytotoxic - classification</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKtvCPwApB4TgELBjO3Y4IKEtpZUqcYGz5dhjYZTEW9upGn49TjfaIxwsz2i-eRq9h9Argj8QTNqP5fG6YUK-k-J9h3GHa_oE7YgUXY05a56i3Ql5js5T-o0x4ZTRM3RGCZaEsR36sx_CpIdKm-zvdfZhqoKrzJJDDg_eVMMyHn6F0kN1iGDmmEJMVb9UY5iC2Xan7Ov9JX0s-mCXT6XSw5J8WsUcgIVYGRiGKsLd7COMMOX0Aj1zekjwcvsv0M-rrz_21_Xt9283-y-3tWFE5Jq4RjrLSWtbqTWT0AmDJbNcODB9y0RDQHNmOkekFaJvJRGU4a4Xpi1kTy_Q26PuIYa7GVJWo0_rNXqCMCclRDGlwey_IGGy4bjjBWRH0MSQUgSnDtGPOi6KYLVmo1bj1Wq8kkI9ZqNoWXu96c_9CPa0tIVR5m-2uU5GDy7qyfh0wgRvJO3agn0-YlBMu_cQVTIeJgO2OGuyssH_-46_q8asTQ</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Brucker, Cosima</creator><creator>Reimann, Jörg</creator><creator>Wagner, Hermann</creator><creator>Kabelitz, Dieter</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Clonal activation of cytotoxic lymphocyte precursors by monoclonal anti-CD3 antibody: analysis of feeder cell requirements</title><author>Brucker, Cosima ; Reimann, Jörg ; Wagner, Hermann ; Kabelitz, Dieter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-1f28fd516d68aa48e97c084d57fecb64721ea54c9f18d77b68173409b7c67c0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Antibody Specificity</topic><topic>Antigens, Differentiation, T-Lymphocyte</topic><topic>Antigens, Surface - immunology</topic><topic>Applied sciences</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Clonal activation</topic><topic>Cytotoxic lymphocyte precursor</topic><topic>Exact sciences and technology</topic><topic>Feeder cell requirement</topic><topic>Humans</topic><topic>Interleukin-2 - pharmacology</topic><topic>Lymphocyte Activation</topic><topic>Lymphocyte Cooperation - drug effects</topic><topic>Monoclonal anti-CD3 antibody</topic><topic>Other techniques and industries</topic><topic>T-Lymphocytes, Cytotoxic - classification</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brucker, Cosima</creatorcontrib><creatorcontrib>Reimann, Jörg</creatorcontrib><creatorcontrib>Wagner, Hermann</creatorcontrib><creatorcontrib>Kabelitz, Dieter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brucker, Cosima</au><au>Reimann, Jörg</au><au>Wagner, Hermann</au><au>Kabelitz, Dieter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clonal activation of cytotoxic lymphocyte precursors by monoclonal anti-CD3 antibody: analysis of feeder cell requirements</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>1987</date><risdate>1987</risdate><volume>14</volume><issue>2</issue><spage>121</spage><epage>125</epage><pages>121-125</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><coden>IMLED6</coden><abstract>Activation of cytotoxic lymphocyte precursors (CLP) by the mitogenic monoclonal anti-CD3 antibody OKT3 was studied under limiting dilution (LD) culture conditions. One out of 2–6 E-rosette-purified T cells gave rise to a cytotoxic T cell (CTL) clone when cultured in the presence of OKT3 (0.2–2 ng/ml), recombinant IL-2 (100 U/ml), and irradiated feeder cells. Clonal CLP activation was optimally supported by a combination of E-rosette-depleted non-T feeder cells with small numbers of T cells added back. Among the cell lines tested, Fc-receptor-bearing monocytic cell lines U937 and HL-60 were efficient feeder cells whereas T cell lines (Jurkat, Molt-4, Ke37) did not support clonal CLP activation. These data indicate that clonal activation of CLP and differentiation into cytotoxic effector cells under LD culture conditions are critically influenced by the type and number of feeder cells used.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3108144</pmid><doi>10.1016/0165-2478(87)90090-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2478 |
| ispartof | Immunology letters, 1987, Vol.14 (2), p.121-125 |
| issn | 0165-2478 1879-0542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77534204 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal - immunology Antibodies, Monoclonal - pharmacology Antibody Specificity Antigens, Differentiation, T-Lymphocyte Antigens, Surface - immunology Applied sciences Cell Line Cells, Cultured Clonal activation Cytotoxic lymphocyte precursor Exact sciences and technology Feeder cell requirement Humans Interleukin-2 - pharmacology Lymphocyte Activation Lymphocyte Cooperation - drug effects Monoclonal anti-CD3 antibody Other techniques and industries T-Lymphocytes, Cytotoxic - classification T-Lymphocytes, Cytotoxic - immunology |
| title | Clonal activation of cytotoxic lymphocyte precursors by monoclonal anti-CD3 antibody: analysis of feeder cell requirements |
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