Role of nucleoside components and internucleotide phosphate groups of oligodeoxyribonucleotide template in its binding to human DNA polymerase α

Affinity labelling of human placenta DNA polymerase α (EC 2.7.7.7) with the reactive oligodeoxyribonucleotide d(pT) 2pC[Pt 2+(NH 3) 2OH](pT) 7 was used for quantitative analysis of enzyme interaction with oligodeoxyribonucleotides as templates. Dissociation constants and Gibb's energy values fo...

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Veröffentlicht in:	FEBS letters 1987-06, Vol.216 (2), p.225-228
Hauptverfasser:	Lavrik, O.I., Levina, A.S., Nevinsky, G.A., Podust, V.N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity labeling Analytical, structural and metabolic biochemistry Biological and medical sciences Deoxyribonucleotides - metabolism DNA Polymerase II - metabolism DNA polymerase α Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Human placenta Humans Kinetics Ligands Oligodeoxyribonucleotide template Oligonucleotides - metabolism Phosphates Placenta - enzymology Protein Binding Structure-Activity Relationship Templates, Genetic Transferases
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