Measurement of the internal pH of mast cell granules using microvolumetric fluorescence and isotopic techniques

The intragranular pH of isolated mast cell granules was measured. Because of the minute amounts of isolated granules available, two techniques were developed by modifying aminoacridine fluorescence and [ 14C]methylamine accumulation techniques to permit measurements with microliter sample volumes. G...

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Veröffentlicht in:	Arch. Biochem. Biophys.; (United States) 1987-04, Vol.254 (1), p.222-233
Hauptverfasser:	De Young, Mary Beth, Nemeth, Edward F., Scarpa, Antonio
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques AMINES Aminoacridines ANIMAL CELLS ANIMALS Applied sciences BASIC BIOLOGICAL SCIENCES CARBON 14 COMPOUNDS Carbon Radioisotopes CONNECTIVE TISSUE CELLS Cytoplasmic Granules - metabolism Exact sciences and technology FLUORESCENCE Hydrogen-Ion Concentration Ions ISOTOPE APPLICATIONS LABELLED COMPOUNDS LUMINESCENCE Male MAMMALS MAST CELLS Mast Cells - metabolism MEASURING METHODS METHYLAMINE Methylamines - metabolism ORGANIC COMPOUNDS Other techniques and industries PH VALUE RATS RESPONSE MODIFYING FACTORS RODENTS SOMATIC CELLS Spectrometry, Fluorescence TRACER TECHNIQUES VERTEBRATES
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